医学图像中粘连细胞分割方法研究

研究粘连细胞图像的分割问题。针对医学细胞图像中,细胞经常发生粘连,细胞粘连部分由于发生像素灰度混合,造成图像不清晰。传统的应用灰度的分割算法由于不能很好的区分粘连部分的灰度,不能完整的分割细胞的问题。为了解决上述问题,提出了自适应阀值调整的粘连细胞分割方法。通过求得粘连部位的细胞像素的具体特征,运用阀值的自适应变化区分粘连部位像素之间的不同,对像素进行划分,克服了传统方法像素划分不清的弊端。实验表明,方法能够有效的分割医学图像中的粘连细胞,取得了比较好的效果。     

Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins

Simulated microgravity (SMG) induced the changes in cell proliferation and cytoskeleton organization, which plays an important factor in various cellular processes. The inhibition in cell cycle progression has been considered to be one of the main causes of proliferation inhibition in cells under SMG, but their mechanisms are still not fully understood. This study aimed to evaluate the effects of SMG on the proliferative ability and cytoskeleton changes of Chang Liver Cells (CCL-13). CCL-13 cells were induced SMG by 3D clinostat for 72 h, while the control group were treated in normal gravity at the same time. The results showed that SMG reduced CCL-13 cell proliferation by an increase in the number of CCL-13 cells in G0/G1 phase. This cell cycle phase arrest of CCL-13 cells was due to a downregulation of cell cycle-related proteins, such as cyclin A1 and A2, cyclin D1, and cyclin-dependent kinase 6 (Cdk6). SMG-exposed CCL-13 cells also exhibited a downregulation of α-tubulin 3 and β-actin which induced the cytoskeleton reorganization. These results suggested that the inhibited proliferation of SMG-exposed CCL-13 cells could be associate with the attenuation of major cell cycle regulators and main cytoskeletal proteins.     

Ozone Contactor Hydrodynamics

Tracer tests were conducted at the 6,000 pounds of ozone per day Tucson, CAP Water Treatment Plant in Tucson, Arizona. The tests were designed to determine T₁₀ values through the contactors at various operating conditions. The tests were modeled using three techniques. Peclet Number was calculated for each of the runs, which would indicate the hydrodynamic conditions inside the ozone contactor. The results indicated that the increase in water flow rate and the number of cells with gas flow increased Peclet Number. The flow rate of liquid seemed to impact the Peclet Number more than gas flow. The headloss in each cell appeared to be important in controlling the distribution of liquid and gas through the cell. A correlation was developed between the product of gas and liquid phase Reynolds Number and Peclet Number.     

痰液显微图像细胞边缘提取的数学形态学新方法

根据痰液细胞图像的边缘灰度变化特点,在经典微分算子检测基础上,运用适当的改进的数学形态学方法对痰液细胞进行边缘提取。在实验中对同一幅痰液细胞图像分别用Sobel算子、Roberts算子、Laplacian算子和基于数学形态学的方法进行边缘检测。结果表明,基于数学形态学的边缘提取算法对于痰液细胞图像边缘提取有很好的效果。     

准分布式光纤Bragg光栅传感器在微生物燃料电池温度场测量中的应用

微生物燃料电池(MFC)在近些年得到了迅猛了发展,尤其是MFC具有产电且同时处理废水的效果,引起了世界各国科学家的高度关注。如何提高MFC产电效率的问题一直是MFC的研究重点,其中温度是影响微生物活性的一个重要因素,进而影响MFC的产电效率。为了能够更好地了解温度场与MFC的产电效率的关系,本文采用准分布式光纤Bragg光栅(FBG)阵列,对MFC阳极室内温度场分布进行实时测量。实验表明:该方法能够测量MFC运行阶段阳极室内温度场分布,反应前后温度差3-4℃,该方法为研究MFC内温度与产电效率的关系提供一种新的手段。