女性护理液抗炎止痒作用的实验研究

女性护理液为中药苦参醇提液加三氯羟基二苯醚复配而成，根据药理方法学以实验动物建立模型，观察护理液的抗炎、止痒作用。结果，单独使用三氯羟基二苯醚，有较好的抗菌消炎作用，但是止痒作用不明显。以苦参醇提液和三氯羟基二苯醚复配，其抗炎、止痒作用均接近地塞米松磷酸钠注射液。     

红芪活性成分免疫调节作用及机制研究进展

免疫功能受损或免疫系统紊乱会导致机体疾病发生,影响人身体健康,基于调节机体免疫治疗疾病已成为临床应用热点。红芪作为甘肃大宗药材,临床应用广泛,具有抗肿瘤、抗炎、免疫调节等多种药理作用。红芪活性成分主要有红芪多糖、黄酮类、萜类、皂苷类等,其中红芪多糖为红芪发挥免疫调节作用主要的物质基础。红芪增强机体免疫力主要体现在增强免疫器官功能,诱导免疫细胞分化、促进免疫因子分泌,提高单核巨噬细胞吞噬功能、调节炎性因子水平以及激活相关通路等方面来达到其抗肿瘤、抗炎作用。本文主要对红芪活性物质免疫调节作用进行阐述,为红芪的进一步临床开发应用提供思路。     

中药黄芪指纹图谱分析研究概况

本文通过对中药黄芪指纹图谱分析应用的综述,阐述了中药指纹图谱分析的概念和特点,介绍了各种有关类型指纹图谱的研究,并指出黄芪及其制剂指纹图谱的分析还应进一步全面深入研究。     

Identification of ages and determination of paeoniflorin in roots of Paeonia lactiflora Pall. From four producing areas based on growth rings

Growth rings were used to determine the root age of medicinal Paeonia lactiflora from four producing areas, and their corresponding paeoniflorin content were measured based on the identification of ages. Different P. lactiflora root samples of different ages were collected from the four major growing areas in China: Bozhou, Anhui Province; Pan'an, Zhejiang Province; Zhongjiang, Sichuan Province; and Heze, Shandong Province. The relationship between the number of growth rings and age was analyzed using hand sections and paraffin sections. The paeoniflorin content in the roots of different P. lactiflora cultivars from different growing areas was measured using high‐performance liquid chromatography (HPLC). The growth rings in the P. lactiflora roots were consistent with the age of the plant from Heze, Zhongjiang, Pan'an, whereas that for the P. lactiflora from Bozhou was one less than the age of the plant. The HPLC results show that the paeoniflorin content was highest in P. lactiflora ‘Baihuachuanshaoyao,’ followed by ‘Baihuahangshaoyao,’ ‘Honghuachuanshaoyao,’ and ‘Honghuahangshaoyao,’ ‘Bozhoushaoyao’ had the lowest levels of paeoniflorin. With increasing age, the paeoniflorin in the roots of the different P.lactiflora cultivars slowly declined or remained the same. In summary, the age of the roots of P. lactiflora from different growing areas can be determined using growth rings. The paeoniflorin content in the roots of P. lactiflora is correlated with cultivar and it was slowly declined with increasing age. Microsc. Res. Tech. 75:1191–1196, 2012. © 2012 Wiley Periodicals, Inc.     

Hydrodistillation condition adjustment for different material particle sizes: a method to increase batch‐to‐batch quality consistency

Varying material particle sizes represent a common contributing factor to the batch‐to‐batch variation of extraction yields. To increase the batch‐to‐batch quality consistency of extracts, a method was proposed to adjust the extraction conditions for different material particle sizes, taking the hydrodistillation extraction process of Radix Curcumae and Fructus Gardeniae as a case study. Statistical models were built for five sesquiterpenes including curcumenone, curcumenol, curdione, curzerenone and furanodienone, to quantitatively describe the effects of particle size and process parameters on their extraction yields. An increase in sodium chloride concentration remarkably increased the yields of curcumenone and curcumenol, and a larger solvent‐to‐solid ratio increased the yields of five sesquiterpenes. Under the adjusted process parameters for two different particle sizes, the yield for each compound was controlled to fall in the 90–110% target range. The proposed method can be applied to various extraction processes of foods and herbal medicines.     

正交法优选玄参中齐墩果酸提取工艺

目的:优选玄参中齐墩果酸的超声提取工艺。方法:使用超声波提取法,采用L9(34)正交设计实验,用紫外分光光度法测定齐墩果酸的含量,确定最佳提取工艺条件。结果:优选出齐墩果酸的最佳提取工艺条件是:超声提取30 min,提取3次,80%乙醇溶液,料液比1∶10。结论:采用此工艺提取方法快速、简单、准确。可作为进一步研究玄参提供参考。     

葛渣总黄酮提取及葛根素含量测定

目的：优化葛渣中总黄酮提取工艺条件,测定葛渣中葛根素含量。方法：以葛渣为原料、总黄酮得率为评价指标,采用L9(34)正交试验设计,考察乙醇体积分数、提取时间、固料比、提取温度对葛渣总黄酮提取效果的影响,同时采用高效液相色谱法(HPLC法)测定葛根素的含量。结果：葛渣中总黄酮的最佳提取工艺条件为以体积分数70%乙醇溶液为溶剂、提取150min、料液比1:40(g/mL)、提取温度80℃,此条件下的总黄酮得率最高,为32.41mg/g。在该提取条件下,葛根、葛渣和葛粉中总黄酮的含量分别为46.11、32.41mg/g和11.23mg/g;葛根、葛渣和葛粉中葛根素的含量分别为40.63、21.24mg/g和4.71mg/g。结论：葛渣中含有丰富的总黄酮和葛根素,该方法可作为从葛渣中提取总黄酮和葛根素的方法。     

基于HPLC-MS/MS法的咽炎片中麦冬掺伪检查

目的 建立咽炎片中麦冬掺伪检测方法.方法 高效液相色谱-串联电喷雾三重四级杆质谱法(HPLC-MS/MS),采用Innoval ODS-2色谱柱(2.1 mm×100 mm,3μm),以乙腈-0.1％甲酸(75:25)为流动相,流速为0.3 ml/min,柱温40℃,进样量2μl,多反应监测(MRM)模式.结果 31批...     

北沙参多糖铁配合物的制备、结构特征及生物活性

目的：制备北沙参多糖及其铁配合物,对其理化性质、结构特征和生物活性进行研究。方法：采用水提醇沉法制备北沙参多糖,经三氯化铁共热合成得到多糖铁配合物,利用紫外-可见光谱、红外光谱、高效阴离子交换色谱、透射电镜、差热-热重联用热分析仪等对多糖及其铁配合物的理化性质和结构特征进行测定,并考察其清除羟自由基和对α-葡萄糖苷酶的抑制作用。结果：北沙参多糖的提取率为18.4%,是由葡萄糖和甘露糖组成,其物质的量比为1.00∶4.25,制备得到的多糖铁配合物中铁离子质量分数为19.31%,是以聚合的β-FeOOH微核为核心,多糖在其表面配合形成的多糖铁（Fe3＋）表面配合物。结果表明,北沙参多糖及其铁配合物均具有清除羟自由基和对α-葡萄糖苷酶的抑制作用,且经铁修饰后的多糖对羟自由基清除作用和对α-葡萄糖苷酶的抑制能力明显增强。结论：北沙参多糖经铁修饰后,对羟自由基清除作用和对α-葡萄糖苷酶的抑制能力均得到提高。     

红芪药渣固态发酵生产蛋白饲料工艺条件优化

以红芪药渣为原料,利用白腐菌（Phanerochaete chrysosporium）/产朊假丝酵母（Candida utilis）混合菌种对其进行固态发酵生产蛋白饲料。采用单因素和正交试验,研究了氮源添加量、装料量、水含量、发酵时间对发酵效果的影响,确定了红芪药渣固态发酵生产蛋白饲料的最佳工艺条件为：（NH4）2SO4添加量30 mg/g干药渣、装料量50%、水分含量2.0 g/g干药渣、发酵时间5 d、发酵温度30 ℃,在上述发酵条件下,发酵产物中真蛋白含量达19.05%,比原来提高了101.16%;粗纤维含量达19.94%,比原来降低了34.79%。利用复合菌固态发酵中药渣,可降低发酵产物中粗纤维含量,同时提高真蛋白含量,为中草药下游产品的开发和应用提供科学依据。