Design of novel urogenital pharmabiotic formulations containing lactobacilli,salivaricin CRL 1328 and non-microbial compounds with different functionalities

Context: The administration of pharmabiotics is a promising alternative to antimicrobial drugs for the treatment and/or prevention of female urogenital infections.

Objective: To design pharmabiotic formulations including bioactive ingredients of microbial origin combined with non-microbial substances and then to evaluate the stability of the combinations during freeze-drying and storage.

Materials and methods: Different formulations including Lactobacillus gasseri CRL 1263, Lactobacillus salivarius CRL 1328, salivaricin CRL 1328 (a bacteriocin) and non-microbial compounds (lactose, inulin and ascorbic acid) were assayed, and the ingredients were freeze-dried together or separately. The formulations were stored in gelatin capsules at 4?°C for 360?d.

Results: The viability of lactobacilli was affected to different extents depending on the strains and on the formulations assayed. L. salivarius and ascorbic acid were successfully combined only after the freeze-drying process. Salivaricin activity was not detected in formulations containing L. gasseri. However, when combined with ascorbic acid, lactose, inulin or L. salivarius, the bacteriocin maintained its activity for 360?d. The selected microorganisms proved to be compatible for their inclusion in multi-strain formulations together with lactose, inulin and ascorbic acid. Salivaricin could be included only in a L. salivarius CRL 1328 single-strain formulation together with non-microbial substances.

Conclusions: This study provides new insights into the design of urogenital pharmabiotics combining beneficial lactobacilli, salivaricin CRL 1328 and compounds with different functionalities.     

低温脂肪酶产生菌的筛选、鉴定及其部分酶学性质

从南极乔治王岛冻土来源的76株低温细菌中筛选到13株低温脂肪酶产生菌，对其中的BTsl0022菌株进行鉴定。通过生理生化特征、16s rDNA基因序列的同源性和系统发育分析发现，菌株RTsl0022属于假单胞菌属(Pseudomonas)，但与已定名的假单胞菌有一定的差异，与未定名的Pseudomonas sp．PsB的亲缘关系最接近，故将其暂定名为Pseudomonas sp．BTsl0022。对该菌脂肪酶的酶学性质初步研究表明，酶的最适作用温度为24℃，对热敏感，60℃处理30min仅残留25％酶活性，酶的适宜作用pH范围在7．0～9．0，最适pH为8．0。     

T8钢－聚有机硅氧烷仿生减粘降阻复合涂层的特性

研究了Ｔ８钢－聚有机硅氧烷仿生减粘降阻复合涂层的表面润湿性、磨料磨损特性及对土壤的减粘降阻性能与金属材料相比，仿生复合涂层的表面憎水性显著提高，水在其表面上的接触角达９２°；与４５钢相比，其磨料磨损的体积相对耐磨系数为６３％，降阻率达１５．２２％～２２．２７％     

具有细小组织的高强度和高延性钢管的开发

介绍了日本川崎制铁公司技术研究所开发出的电焊钢管采用热张力减径工艺的特性 ,及这种钢管的高强度、高延性等基本特性。用这种钢管制造的汽车零部件 ,经弯曲、冲击和疲劳特性等实验 ,证明具有良好的机械加工性能 ,是制造汽车用零部件的优良材料。     

黄河黑山峡河段大柳树水库比小观音水库原始库容多40亿m3的作用

一级开发大柳树高坝原始库容比二级开发小观音高坝多 4 0亿m3 ,库容大带来的好处体现在 5～ 7月可多向下游增供水量 ,缓解下游河道断流 ;延长泄放清水时间 ;增加发电效益 ;满足宁蒙河段防凌要求 ;增加汛期集中泄水 ,减缓下游河道淤积萎缩 ;满足南水北调西线工程水量调节要求等。为了更好优化配置黄河水资源 ,从全局和长远利益考虑 ,应采用一级开发方案 ,修建大柳树高坝大库     

UV-LIGA深度光刻机平滑衍射技术研究

UV-LIGA深度光刻技术是加工微型机电系统的一项重要的微细加工技术。介绍了一种平滑衍射效应的位错强度叠加原理，它采用了蝇眼积分透镜去平滑衍射，并模拟数值计算了硅片表面的光强分布，最后给出了光刻结果。     

The Use of Biopreservatives in the Control of Bacterial Contaminants of Sugarcane Alcohol Fermentation

ABSTRACT: Efficacy and safety of biopreservatives were studied for microbiological control of alcohol fermentation. Minimum inhibitory concentrations of nisin and lysozyme, and combinations with EDTA and Tween 20, were determined for Lactobacillus plantarum, L. fermentum , and L. confusus. The highest MIC value for nisin was 9.0 ppm for L. fermentum. Lysozyme was efficient against L. plantarum (700 ppm). A 2⁴ factorial design was used to study the effect of biopreservatives and potentiators on the growth kinetics of Lactobacillus fermentum. The combination of 8.6 ppm of nisin plus 0.1% of Tween 20 delayed the lag phase by 12 h. The biopreservatives did not affect the fermentation kinetics of Saccharomyces cerevisiae.     

一种新型声波降粘防蜡装置

新型声波降粘防措装置由上、下接头、振动簧片和收缩喷嘴构成。装置安装在抽油泵泵简之下，尾管之上，在压差作用下井下流体以较大速度从装置的喷嘴射出，冲击振动簧片，产生机械振动声波，油水混合液在声波中产生相应的声波力学效应和分散乳化作用等，使混合液在油管内的粘度降低，蜡晶不易聚集长大，从而达到降粘防蜡延长油井热洗周期的目的。室内试验及现场近20口井的应用情况表明，这种装置具有结构简单、成本低廉、应用方便等特点，具有明显的降粘防蜡效果。     

硅化腐植酸钠GFN-1的研制

针对目前生产腐植酸硅化产品原料受限的缺点,在大量试验的基础上,确定了一条以腐植酸和硅化剂为主要原料的生产工艺,合成了一种与有机硅腐植酸钾(OSAM-K)性能相当的硅化腐植酸钠产品GFN-1,对其常规性能指标和在清水及无固相聚合物钻井液体系中、在膨润土浆和低固相钻井液体系中应用指标,耐温性能进行了对比实验。实验结果表明,GFN-1作为钻井液添加剂,具有良好的降滤失、降黏、耐温及热稳定性,并兼有优良的控制页岩分散能力,其大多数应用性能相当于或略优于OSAM-K,作为替代品完全能够满足油田现场应用的需要。GFN-1的硅化原料性质稳定、来源广泛,生产工艺简单易行,产品质量稳定,具有较好的推广价值。     

优化改造油田生产工艺 增产增效提高环保水平——涠洲11-4油田生产工艺优化改造实践

油田生产水处理和海管输油能力不足是制约涠洲11-4油田生产和影响生产安全的瓶颈问题。合理利用油田原有设施和条件,对生产水处理系统和输油管道系统进行工艺优化改造,使该油田B平台海管运行的安全可靠性得以明显提高,并使油田生产水处理能力和处理质量得到有效改善,同时大幅度降低了油田生产成本,拓宽了油田增产挖潜的空间。涠洲11-4油田优化改造生产工艺实践经验,可为进一步拓宽海上老油气田的生产管理思路提供参考。