Effect of freeze-drying process on the physical stability and properties of Voriconazole complex system

This paper studied the effect of lyophilization together with annealing on the physical properties, such as critical micelle concentration, particle size, differential scanning calorimetry, and eutectic point. The freeze-drying method had successfully set up the Voriconazole complex system. Samples processed by lyophilization had slightly superiority compared to those without the lyophilization in terms of surface properties. Furthermore, this paper also investigated the physical stabilities of Voriconazole complex system related to cloud point thermodynamic and insoluble particles. Samples processed by lyophilization with annealing achieved better performance of physical stabilities than those without any process.     

Role of voriconazole in combined acanthamoeba and fungal corneal ulcer

We report a case of a 21 year old male who presented with smear-proven fungal corneal ulcer in left eye, refractory to treatment with topical natamycin. Corneal smears and culture were taken along with anterior chamber tap for microbiological investigations and intracameral 1% voriconazole was injected followed by institution of oral as well as topical voriconazole drops. Corneal and aqueous smear as well as culture confirmed the presence of acanthamoeba. The keratitis responded favourably to voriconazole therapy which was instituted along with topical trophicidal drugs and anterior chamber became quiet by 7th day. The patient was slowly tapered from voriconazole and was off the drug by 6 months. At one year follow up, he continues to remain asymptomatic. The possibility exists that the fungal organisms had either partially or completely responded to natamycin eye drops and voriconazole therapy further inhibited their growth along with being highly efficacious in suppressing acanthamoeba trophozoites. Voriconazole may be specifically instrumental in corneal ulcers with coinfection of acanthamoeba and fungal organisms and preventing recrudescence of both. Normal saline wet mount and 10% KOH mount of paracentesis sample for suspicious cases of refractory corneal ulcers to specifically look for trophozoites in order to institute timely treatment are recommended.     

伏立康唑PLGA纳米微粒的制备及其形态和释放特点分析

目的制备伏立康唑PLGA纳米微粒,并分析其形态及释放特点。方法采用乳化-溶剂挥发法制备伏立康唑PLGA纳米微粒,用激光粒径分析仪及扫描电镜分别进行分散性和形态学分析,经HPLC分析其载药量及释放特点。结果经激光粒径分析,样品粒径峰值为(126±20)nm,分布指数为1.5。电镜观察颗粒分布均匀,表面光滑。HPLC分析载药量为(1.9±0.6)%,包封率约为12%,24h内存在突释,第2天到第8天缓慢释放。结论已成功制备了伏立康唑PLGA纳米微粒,能实现缓慢释放,减少给药次数的目的。