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Yuhao Du Charles E. Hendrick Krysta S. Frye Dr. Lindsay R. Comstock 《Chembiochem : a European journal of chemical biology》2012,13(15):2225-2233
Azide and alkyne‐functionalized N‐mustard analogues of S‐adenosyl‐L ‐methionine have been synthesized and were demonstrated to undergo efficient methyltransferase‐dependent DNA alkylation by M.TaqI and M.HhaI. Subsequent labeling of the DNA with a fluorophore was carried out using copper‐catalyzed azide–alkyne cycloaddition chemistry and was visualized by fluorescence scanning. This work demonstrates the utility of functionalized N‐mustard analogues as biochemical tools to study biological methylation and offers a facile way to site‐selectively label substrates of DNA methyltransferases. 相似文献
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