纳米SiO_2与有机物分子的亲和性和分散性

介绍纳米SiO2的合成和粒子表面的粗糙形貌以及纳米粒子链具有类似高分子链的弹性力学属性,研究纳米SiO2的亲合性,探讨亲合性对纳米SiO2的分散、改性、应用的影响。研究表明,在极性外加剂(如乙二胺、乙二醇、PEG、PDMS、PTHF、PMMA、PBMA等)存在的情况下,纳米SiO2的弹性粒子链和硅烷醇对SiO2的分散和改性具有显著的影响。带有大烷基侧链的高分子链与SiO2表面的亲合能较低,而带有官能团的高分子链与SiO2表面具有较高的亲合性。     

Thermodynamic state of reinforced interpenetrating polymer networks

The free energies of mixing of two networks in the interpenetrating polymer network based on crosslinked polyurethane and poly(ester acrylate) have been determined by the vapour sorption method. It was established that the constituent networks in the IPN are not miscible. The introduction of fillers of different chemical nature increases the compatibility. The thermodynamic affinity of the fillers to the individual networks and IPN was estimated. It was established that when the free energy of interaction of one or both components of the IPN with the filler is negative, reinforcement leads to the formation of a compatible and equilibrium system. For fillers having no affinity to the polymers, compatibilization is observed, which is connected with slowing down of phase separation in the system in the presence of filler.     

Complementarity determining region residues aspartic acid at H55, serine at H95 and tyrosines at H97 and L96 play important roles in the B72.3 antibody-TAG72 antigen interaction

Structural analysis derived from the crystallographic studyof the chimeric B72.3 antibody illustrated some major atomicinteractions between complementarity determining region (CDR)residues. For example, hydrogen bonds are formed between H35/H95,L50/H97, H53/H55 and H96/L96 respectively. These CDR residuesmay play important roles in the B72.3–TAG72 (antibody-antigen)interaction either by direct interaction with the TAG72 antigenor by maintaining a CDR loop conformation through atomic interactionsbetween CDR residues. In order to confirm these assumptions,we altered these CDR residues by site-directed mutagenesis anddetermined binding affinities of these mutant chimeric antibodiesfor the TAG72 antigen in a solid-phase radioimmunoassay. Wefound that H55, H95, H97 and L96 are important CDR residuesfor the B72.3–TAG72 interaction. Single amino acid substitutionsof aspartic acid and serine by alanine at H55 of CDR2 and atH95 of CDR3 respectively and of tyrosine by phenylalanine atH97 and L96 of CDR3, significantly reduced the binding affinityfor the TAG72 antigen by 20-, 8-, 16- and 45-fold respectively.Therefore, this study reveals some of the requirements for maintainingthe integrity of the B72.3 antibody combining sites.     

四种金属离子亲和层析纯化重组戊型肝炎病毒包涵体的效果比较

目的 选择固相金属亲和层析柱的金属离子,以优化分离纯化重组戊型肝炎病毒包涵体的条件。方法 在同一试验条件下用4种金属离子柱分离纯化目的蛋白。结果 包涵体经不同离子柱层析时目的蛋白的收获率、纯度、洗脱条件各异。结论 镍离子柱纯化效果最佳。     

抗rHu-IFN-αMcAb亲和常数的测定

采用ELISA间接法检测抗原－抗体反应系统中游离抗体量的方法，对本实验室制备的5系抗rHu－IFN－αMcAb进行了亲和力测定，获得比较准确的McAb亲和常数。     

基于仿射传播的有向网络聚类算法

现有大多数的网络聚类方法都只是针对无向网络, 已有的有向网络聚类方法建立在传统聚类算法基础之上, 存在着一定的局限性。针对上述问题, 提出一种基于仿射传播的有向网络聚类算法, 该算法首先采用SimRank作为节点之间的相似度, 并将计算得到的结果转换为适应于仿射传播算法的负值; 然后将相似度矩阵作为输入, 利用具有更好性能的仿射传播算法对有向网络进行聚类。实验结果表明, 所提出算法的聚类性能优于其他几种具有代表性的有向网络聚类算法。     

Cellulose-binding domains: potential for purification of complex proteins

The endoglucanase CenA and the exoglucanase Cex from Cellulomonasfimi each contain a discrete cellulose-binding domain (CBD),at the amino-terminus or carboxyl-terminus respectively. Thegene fragment encoding the CBD can be fused to the gene of aprotein of interest. Using this approach hybrid proteins canbe engineered which bind reversibly to cellulose and exhibitthe biological activity of the protein partner. Alkaline phosphatase(PhoA) from Escherichia coli, and a ß-glucosidase(Abg) from an Agrobacterium sp. are dimeric proteins. The fusionpolypeptides CenA-PhoA and Abg-CBC_cex are sensitive to proteolysisat the junctions between the fusion partners. Proteolysis resultsin a mixture of homo- and heterodimers; these bind to celluloseif one or both of the monomers carry a CBD, e.g. CenA-PhoA/CenA-PhoAand CenA-PhoA/PhoA. CBD fusion polypeptides could be used inthis way to purify polypeptides which associate with the fusionpartner.     

基于近邻消息传递的自适应局部线性嵌入

针对局部线性嵌入(LLE)降维算法中邻域参数的人工设定及其全局性的缺陷,研究了聚类和降维的内在联系,提出了邻域参数的自适应选取策略,构建了一种聚类和降维的自适应局部线性嵌入(ALLE)算法,为每个样本点设计最佳的近邻搜索空间,自适应选取邻近点计算权值重建矩阵,基于类信息重新定义了重构误差函数。实验表明,新算法更能体现出数学上流形概念的局部坐标化本质,对不相关数据、冗余数据和噪声数据具有良好的鲁棒性,在实际识别问题中体现出优越的性能。     

一种监控多核处理器系统核状态的方法

本文主要针对目前多核处理器中核状态难以监控这一问题,分别从硬件和软件角度给出了解决方案,并通过具体的实现方法和试验说明了使用软件方法解决此问题的优越性。     

静态吸附法评估膜对油的亲和性

膜对油的亲和性是表征膜的重要指标之一。本文以油粒在微滤膜上吸附性污染的机理及影响因素为基本依据，实验测定了亲油或亲水性不同的几种微滤膜的静态吸附量。研究表明，该吸附量与膜的亲油或亲水性具有明显的关系，膜对油的静态吸附量可表征微滤膜对油的亲和性，该方法简便、实用。