21~28GHz波段平衡式放大器

采用OMM IC的0.2μm PHEM T工艺设计了工作在21~28 GH z的平衡式放大器。正交耦合电桥采用兰格电桥。兰格电桥和平衡式放大器的在片测试结果和仿真结果基本吻合,平衡式放大器在21~28 GH z的增益为18~20 dB,输入和输出回波损耗小于-20 dB,在26 GH z处的输出1 dB压缩点功率为21 dBm。     

施肥模型研究综述

对施肥模型和参数研究的历史、现状和趋势进行了综述,并指出今后定量施肥研究的关键问题。     

多人决策的一类相对均衡解

本文分析由于决策人偏好不同而引起决策差异的多人多目标决策问题，提出了这类决策问题的一类相对均衡解的概念和求取方法。相对均衡解以决策群体偏好的加权集结为基础，能最大程度地反映每个决策人的偏好。文中研究相对均衡解的ＳＷＴ求取方法，并给出了求解的步骤。     

Relative Contributions of the Male and the Female to Sexual Behavior and Reproductive Success in the Japanese Quail (Coturnix japonica).

Two contrasting investigative techniques provided evidence consistent with the interpretation that female quail (Coturnix japonica) regulate male copulatory behavior by the duration of their immobility and through this behavioral mechanism they also control the rate of fertilization of their eggs. In Experiment 1, copulation tests with males and females from different genetic lines showed that the type of female that participated in a copulatory test significantly influenced the latency of the male's grab, mount, and cloacal contact responses and also determined the efficiency of the male's copulatory behavior. These measures of male performance were correlated with female immobility in Experiment 2, which used a more homogeneous population of quail. Furthermore, 2 of these measures (copulatory efficiency and the latency to make cloacal contact) were correlated with fertilization rate. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

基于CMOS模拟开关实现平衡混频器

详细介绍了有源单平衡混频器的电路组成,分析了有源平衡混频器的工作原理。基于CMOS模拟开关设计实现了一种(低功耗、高线性度的)开关平衡混频器,最后对混频器的指标进行了测量和分析。测试结果表明(在3.3V电源电压下,消耗电流小于10mA,射频输入信号)在60～110MHz频带范围内,(本振信号+10dBm时)插入损耗小于7dB,波动小于1dB,输入P1dB压缩点大于13dBm。     

均衡化特征匹配的非刚体细胞形态跟踪

提出利用均衡化特征匹配来进行非刚性细胞形体跟踪的方法。采用重启动的随机游走方法建立并求解特征匹配概率模型,利用双向均衡方法对匹配邻接矩阵进行均衡化处理,得到指定目标与待跟踪目标之间的精确匹配,以获得目标的定位跟踪结果。同时利用特征匹配结果进行目标的自动标定,并应用图像分割方法进行目标的精确轮廓跟踪。实验结果表明,将该方法应用于视频中动态背景下的运动细胞形态跟踪时,在背景相似度较高及目标迅速移动的条件下,表现出了良好的性能,与同类方法相比可获得较高的定位精度以及更为准确的目标轮廓。     

One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis

Xenoantigens cause hyperacute rejection and limit the success of interspecific xenografts. Therefore, genes involved in xenoantigen biosynthesis, such as GGTA1, CMAH, and B4GALNT2, are key targets to improve the outcomes of xenotransplantation. In this study, we introduced a CRISPR/Cas9 system simultaneously targeting GGTA1, CMAH, and B4GALNT2 into in vitro-fertilized zygotes using electroporation for the one-step generation of multiple gene-edited pigs without xenoantigens. First, we optimized the combination of guide RNAs (gRNAs) targeting GGTA1 and CMAH with respect to gene editing efficiency in zygotes, and transferred electroporated embryos with the optimized gRNAs and Cas9 into recipient gilts. Next, we optimized the Cas9 protein concentration with respect to the gene editing efficiency when GGTA1, CMAH, and B4GALNT2 were targeted simultaneously, and generated gene-edited pigs using the optimized conditions. We achieved the one-step generation of GGTA1/CMAH double-edited pigs and GGTA1/CMAH/B4GALNT2 triple-edited pigs. Immunohistological analyses demonstrated the downregulation of xenoantigens; however, these multiple gene-edited pigs were genetic mosaics that failed to knock out some xenoantigens. Although mosaicism should be resolved, the electroporation technique could become a primary method for the one-step generation of multiple gene modifications in pigs aimed at improving pig-to-human xenotransplantation.     

Mineral nutrition of slash pine in subtropical Australia. II. foliar nutrient response to fertilization

In the previous paper, we reported the stand growth of slash pine (Pinus elliottii) during the first 11.5 years of plantation in response to (1) initial fertilization at plantation establishment with P rates of 11, 22, 45 and 90 kg P ha^–1 which were either banded or broadcast in the presence or absence of basal fertilizers containing 50 kg N ha^–1, 50 kg K ha^–1 and 5 kg Cu ha^–1 and (2) application of additional 40 kg P ha^–1 at age 10 years. Here we present the responses in foliar nutrient concentrations of slash pine in the first 11.5 years to the initial fertilization and the additional P applied at age 10 years.Foliar N and K concentrations in the first 9.6 years of plantation decreased with the initial P rate. Application of the basal fertilizers improved foliar Cu concentration. Foliar Ca and Mg concentrations increased linearly with the initial P rate. The initial fertilization did not affect foliar Mn concentration in the first 9.6 years. Foliar P concentration increased quadratically with the initial P rate, which accounted for 77–86% of the variation in foliar P concentration. Most of the explained variation in foliar nutrient concentrations was attributable to the plantation age except for foliar P concentration. In the case of foliar P concentration, 53% was explained by the initial P rate, 31% by the plantation age and 2% by the positive interaction between the initial P rate and the plantation age. Foliar P concentration of slash pine at age 11.5 years increased quadratically with the initial P rate and linearly with the additional 40 kg P ha^–1 applied at age 10 years, accounting for 81% of the variation in the foliar P concentration. Foliar nutrient analysis indicated that P was the major limiting nutrient affecting the stand growth of slash pine in the first 11.5 years.     

A mathematical model for predicting the soil nitrogen status under varying fertilizer practices in a continuous cropping sequence

A mathematical model has been proposed for predicting the changes in soil nitrogen status due to continuous fertilization in a continuous cropping sequence. The model also enabled the prediction of the steady state of soil nitrogen for a specified fertilizer practice.The model was applied to six years nitrogen availability data of four fertilizer practices in finger millet-maize-cowpea sequence followed in the Long Term Fertilizer Experiments conducted at Tamil Nadu Agricultural University, Coimbatore, India. The agreement between the predicted soil nitrogen status by the model and the actuals was proved by employing reliability index.     

Balanced pasture fertilization in the Basque Country: I. Phosphorus and potassium budgets on dairy farms

Dairy farming is the main agricultural activity of the Basque Country. A dairy farm is characterized as a system with soils and crops, forage, cattle and manure as main components, and in such a system, nutrient cycling is very important to maintain soil fertility and optimize forage production. To quantify nutrient transfers in the cycle, a simple system was developed and has been applied to seventeen farms to examine its ability to achieve a balanced P and K fertilization. These farms have provided data on inputs (fertilizer, feeds, concentrates), pasture and manure management, and outputs (milk production), and soil samples have been taken from farm pastures. Phosphorus and K in excreta and uneaten pasture is used with a relatively high efficiency as suggested by the relatively high efficiency of P and K utilization by the pasture that usually ranges from 70 to 90%. Concentrate feeding (3000 kg cow^–1 yr^–1) represents one of the main P and K inputs in Basque Country dairy farms, averaging 26 and 66 kg ha^–1, respectively. Besides, release of K in the soil through slow liberation from non-exchangeable sites was estimated as 30 kg ha^–1. Thus, a high efficiency in excreta recycling would diminish substantially P and K mineral fertilizer needs. Farm nutrient budgets appear to be a convenient tool for determining nutrient shortages and surpluses at farm level, and thus they are considered as a first step to support a better management of maintenance fertilization of permanent pastures.