用微弧氧化技术处理医用钛合金表面的研究

采用微弧氧化对钛合金表面改性，讨论在不同条件下微弧氧化膜的形貌、结构和成份。结果表明，在钛合金表面得到一层金红石型TiO2和锐钛矿型TiO2的多孔氧化膜，且在膜中可以渗入钙，磷离子。膜层中的元素呈阶梯分布，外层的Ca,P含量较大，Ca/P含量随溶液中Ca/P含量近似呈线性增强。在确定的电解液下表面孔隙率基本不变，但孔洞大小随反应电压的增加而增加；膜的生长速率随反应时间的延长呈先快后慢变化。这种含有Ca，P成份的多孔表层结构有利于骨细胞的吸附和结合。     

3D Printed SiOC(N) Ceramic Scaffolds for Bone Tissue Regeneration: Improved Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

Bone tissue engineering has developed significantly in recent years as there has been increasing demand for bone substitutes due to trauma, cancer, arthritis, and infections. The scaffolds for bone regeneration need to be mechanically stable and have a 3D architecture with interconnected pores. With the advances in additive manufacturing technology, these requirements can be fulfilled by 3D printing scaffolds with controlled geometry and porosity using a low-cost multistep process. The scaffolds, however, must also be bioactive to promote the environment for the cells to regenerate into bone tissue. To determine if a low-cost 3D printing method for bespoke SiOC(N) porous structures can regenerate bone, these structures were tested for osteointegration potential by using human mesenchymal stem cells (hMSCs). This includes checking the general biocompatibilities under the osteogenic differentiation environment (cell proliferation and metabolism). Moreover, cell morphology was observed by confocal microscopy, and gene expressions on typical osteogenic markers at different stages for bone formation were determined by real-time PCR. The results of the study showed the pore size of the scaffolds had a significant impact on differentiation. A certain range of pore size could stimulate osteogenic differentiation, thus promoting bone regrowth and regeneration.     

In vivo biocompatibility and bioactivity of in situ synthesized hydroxyapatite/polyetheretherketone composite materials

Hydroxyapatite/polyetheretherketone (HA/PEEK) composite materials were prepared via an in situ synthesis process in order to achieve strong bonding between PEEK matrix and hydroxyapatite fillers, and ultimately to improve the mechanical properties of the composites. In the study, the biocompatibility of the synthesized HA/PEEK materials was investigated by acute toxicity test, hemolytic test, sensitization test, pyrogen test, intradermal test, and toxicity assay test on animal tissue and cells for the purpose of examining the possible adverse effects of the residue organic chemicals from the in situ synthesis process. In vivo bioactivity of both lab‐synthesized PEEK and HA/PEEK composites with various HA content was also studied. It is found that the in situ synthesized composite materials possess good biocompability without toxicity. Although the bioactivity of the material increases with HA content, the composite material with 5.6 vol % HA exhibits satisfactory bioactivity without compromising its excellent mechanical performance, which hints to a potential use as load‐bearing orthopedic material. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013     

珍珠粉改性聚丙烯单丝的制备及生物性能

为提高盆底修复补片用聚丙烯单丝的生物相容性,制备了聚丙烯/珍珠粉共混单丝。对纺丝工艺、珍珠粉在聚丙烯单丝表面的分散情况、改性单丝的力学性能及生物相容性进行了研究。结果显示,聚丙烯/珍珠粉共混单丝可纺性良好,珍珠粉的加入没有影响单丝的力学性能;SEM观察显示,珍珠粉在单丝表面大量分布;与不含珍珠粉聚丙烯单丝相比较,珍珠粉改性的聚丙烯单丝有更好的细胞增殖性能,说明珍珠粉填充的聚丙烯单丝作为补片原料植入人体时可望有更好的生物相容性。     

丝素膜的制备及其用于细胞培养

通过正交试验确定了制备纯丝素膜的最佳工艺参数：搅拌温度50℃，溶液中丝素蛋白质量分数15％，干燥温度80℃．将该条件下所得膜用于肝细胞培养，实验表明细胞在丝素膜上生长良好，功能性指标较传统培养等同或较好，丝素膜在功能性细胞培养基质等领域具有良好的应用前景．