Biophysical Model of Larval Yellow Perch Advection and Settlement in Lake Michigan

Potential for large-scale physical transport processes to affect recruitment of Lake Michigan yellow perch (Perca flavescens) was studied by examining the variation in larval distribution, growth rate, and settlement during June–August 1998–2003 using a 3D particle transport model linked with an individual-based bioenergetics growth model. In all years, virtual larvae were released nearshore in southwestern Lake Michigan, a known and important spawning region for yellow perch. For any given year, the same circulation pattern and water temperature either promoted or reduced yellow perch settlement depending on the consumption rates and settlement size chosen in the growth model. Increased consumption increased the number of settled larvae and expanded the total area where larvae settled, whereas increased settlement size reduced the number of settled larvae and reduced the overall settlement area. Interannual variability in circulation patterns and water temperature also resulted in contrasting larval settlement rates, settlement locations, and size of settlement areas between years. Model predictions were most consistent with field observations of age-0 yellow perch from Illinois and Michigan waters when settlement was assumed to occur at 50 mm. Moreover, our model suggests that larvae originating from southwestern Lake Michigan can recruit anywhere within the southern basin and even in the northern basin. Future model improvement will require information on the relative contribution of various sectors to the larval pool, their distribution with reference to the hydrodynamic landscape, the feeding and growth of yellow perch during their pelagic phase, and the size at transition to demersal stage.     

Sensitivity of juvenile salmonid growth to future climate trends

Seasonal temperature and bioenergetic models were coupled to explore the impacts on juvenile salmonid growth of possible climate‐induced changes to mean annual water temperature and snowpack in four characteristic ecoregions. Increasing mean temperature increases juvenile growth in streams that currently experience cool spring temperatures. In streams with currently warm spring temperatures, an increase shortens the duration of optimal conditions and truncates growth. A loss of snow enhances growth in cool‐summer streams and decreases growth in warm‐summer streams. The relative impacts of such climate change trends will vary significantly across ecoregions. Copyright © 2010 John Wiley & Sons, Ltd.     

Platelet Mitochondrial Bioenergetics Reprogramming in Patients with Urothelial Carcinoma

Mitochondrial bioenergetics reprogramming is an essential response of cells to stress. Platelets, an accessible source of mitochondria, have a crucial role in cancer development; however, the platelet mitochondrial function has not been studied in urothelial carcinoma (UC) patients. A total of 15 patients with UC and 15 healthy controls were included in the study. Parameters of platelet mitochondrial respiration were evaluated using the high-resolution respirometry method, and the selected antioxidant levels were determined by HPLC. In addition, oxidative stress was evaluated by the thiobarbituric acid reactive substances (TBARS) concentration in plasma. We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced the endogenous platelet coenzyme Q₁₀ (CoQ₁₀) concentration in UC patients. The activity of citrate synthase was decreased in UC patients vs. controls (p = 0.0191). γ-tocopherol, α-tocopherol in platelets, and β-carotene in plasma were significantly lower in UC patients (p = 0.0019; p = 0.02; p = 0.0387, respectively), whereas the plasma concentration of TBARS was increased (p = 0.0022) vs. controls. The changes in platelet mitochondrial bioenergetics are consistent with cell metabolism reprogramming in UC patients. We suppose that increased oxidative stress, decreased OXPHOS, and a reduced platelet endogenous CoQ₁₀ level can contribute to the reprogramming of platelet mitochondrial OXPHOS toward the activation of glycolysis. The impaired mitochondrial function can contribute to increased oxidative stress by triggering the reverse electron transport from the CoQ₁₀ cycle (Q-junction) to CI.     

Modelling Temperature,Body Size,Prey Density,and Stream Gradient Impacts on Longitudinal Patterns of Potential Production of Drift‐Feeding Trout

In this study, we modelled idealized stream reaches using empirical hydrodynamic and bioenergetic parameters to predict how rainbow trout production depends on physical and biological variations across a downstream gradient, and we compared these downstream effects in a low and high‐gradient stream reach. We found that longitudinal production potential (i.e. net rate of energetic intake per 100 m of stream length) generally increased with increasing stream size when stream gradient was low. This was not the case, however, for high‐gradient streams, wherein maximum longitudinal production potential was associated with middle or low stream size (Q_MAD = 2.5 to 25 m³ s^?1). Areal production potential (net rate of energetic intake per m² of wetted stream bed) reached a maximum at low stream size (Q_MAD = 2.5 m³ s^?1) with both high and low gradients. We also showed that high stream temperature and low drift density could potentially cause adult rainbow trout to be excluded from stream reaches with high flow. The models presented here have a stronger mechanistic basis for predicting fish production across heterogeneous stream environments and provide more nuanced predictions in response to variation in environmental features than their physical habitat‐based predecessors. Copyright © 2016 John Wiley & Sons, Ltd.     

Mitochondrial Mechanisms in Septic Cardiomyopathy

Sepsis is the manifestation of the immune and inflammatory response to infection that may ultimately result in multi organ failure. Despite the therapeutic strategies that have been used up to now, sepsis and septic shock remain a leading cause of death in critically ill patients. Myocardial dysfunction is a well-described complication of severe sepsis, also referred to as septic cardiomyopathy, which may progress to right and left ventricular pump failure. Many substances and mechanisms seem to be involved in myocardial dysfunction in sepsis, including toxins, cytokines, nitric oxide, complement activation, apoptosis and energy metabolic derangements. Nevertheless, the precise underlying molecular mechanisms as well as their significance in the pathogenesis of septic cardiomyopathy remain incompletely understood. A well-investigated abnormality in septic cardiomyopathy is mitochondrial dysfunction, which likely contributes to cardiac dysfunction by causing myocardial energy depletion. A number of mechanisms have been proposed to cause mitochondrial dysfunction in septic cardiomyopathy, although it remains controversially discussed whether some mechanisms impair mitochondrial function or serve to restore mitochondrial function. The purpose of this review is to discuss mitochondrial mechanisms that may causally contribute to mitochondrial dysfunction and/or may represent adaptive responses to mitochondrial dysfunction in septic cardiomyopathy.     

A thermodynamic analysis of the activated sludge process: Application to soybean wastewater treatment in a sequencing batch reactor

A bioenergetic methodology was integrated with a modified activated sludge model No.1 (ASM1) to analyze the activated sludge process, with the treatment of soybean‐processing wastewater as an example. With the bioenergetic methodology established by McCarty and coworkers, the microbial yield was predicted and the overall stoichiometrics for biological reactions involving the key chemical and biological species in activated sludge were established. These obtained parameters were related to the ASM1 model, which was modified after coupling the biological reactions in activated sludge with electron balances. This approach was able to approximately describe the treatment of soybean wastewater by activated sludge in a sequencing batch reactor in terms of substrate utilization, biomass growth, and the elector acceptor consumption. Such an attempt provides useful information for accurate modeling of the complex activated sludge process. © 2009 American Institute of Chemical Engineers AIChE J, 2009     

The influence of diet, consumption and lipid use on recruitment of white bass

The abundance of white bass (Morone chrysops) in Lake Erie has declined in recent years, sparking interest in mechanisms influencing its recruitment. We evaluate two mechanisms affecting recruitment: diet and the potential for competition, and storage of lipid energy reserves and the relationship to overwinter survival. The fish in our study were characteristic of white bass in the northern portion of their range, feeding predominantly on zooplankton. Only the largest age‐0 white bass ate fish as a significant portion of their diet. Over the summer sampling period, we found decreasing ration sizes, expressed as a percentage of maximum ration, as the summer progressed with a concomitant decrease in the relative amount of lipid storage. In laboratory experiments, age‐0 white bass held at 5°C and given food ad libitum did feed, but at rates that were insufficient to maintain body weight. Loss in weight was accompanied with a loss in lipids at a rate of 2.8 mg of lipids per gram of body weight per day. Based on our data, we concluded that age‐0 white bass in Lake Erie were food‐limited. Food limitation resulted in reduced growth rates, presumably related to competition with other planktivorous fishes. Reduced growth results in increased mortality and, ultimately, low recruitment through increased risk of predation by larger piscivorous fishes, reduced ability for white bass to switch to more energetically profitable piscivory and the increased likelihood of higher overwinter mortality because of reduced lipid stores.     

Adenylate Kinase and AMP Signaling Networks: Metabolic Monitoring,Signal Communication and Body Energy Sensing

Adenylate kinase and downstream AMP signaling is an integrated metabolic monitoring system which reads the cellular energy state in order to tune and report signals to metabolic sensors. A network of adenylate kinase isoforms (AK1-AK7) are distributed throughout intracellular compartments, interstitial space and body fluids to regulate energetic and metabolic signaling circuits, securing efficient cell energy economy, signal communication and stress response. The dynamics of adenylate kinase-catalyzed phosphotransfer regulates multiple intracellular and extracellular energy-dependent and nucleotide signaling processes, including excitation-contraction coupling, hormone secretion, cell and ciliary motility, nuclear transport, energetics of cell cycle, DNA synthesis and repair, and developmental programming. Metabolomic analyses indicate that cellular, interstitial and blood AMP levels are potential metabolic signals associated with vital functions including body energy sensing, sleep, hibernation and food intake. Either low or excess AMP signaling has been linked to human disease such as diabetes, obesity and hypertrophic cardiomyopathy. Recent studies indicate that derangements in adenylate kinase-mediated energetic signaling due to mutations in AK1, AK2 or AK7 isoforms are associated with hemolytic anemia, reticular dysgenesis and ciliary dyskinesia. Moreover, hormonal, food and antidiabetic drug actions are frequently coupled to alterations of cellular AMP levels and associated signaling. Thus, by monitoring energy state and generating and distributing AMP metabolic signals adenylate kinase represents a unique hub within the cellular homeostatic network.     

The future of habitat modeling and instream flow assessment techniques

This paper examines emerging trends in applied instream flow assessment methods within the context of an ecologically based assessment framework, in light of the challenges imposed by the spatial and temporal domains of aquatic ecosystems. I will attempt to highlight what I consider to be the more promising technologies, modeling techniques and analysis approaches that represent workable tools to meet the needs of practical, applied instream flow assessments. To this end, I will touch on measurement techniques and technologies used to characterize the spatial domain of river systems, analysis tools for characterization of the hydrodynamic elements of rivers in both the spatial and temporal domains, and finally tools and approaches which integrate the biological elements at the individual, population and community levels. Much of my view of the future of habitat modeling remains an abstraction, in that integration of all the pieces has yet to be accomplished, field validation remains unproven, availability of an integrated analysis framework (i.e. computer software system) is not yet available, and a clear framework for selection and application of specific tools has not been developed. However, in presenting this particular view of the future, I hope to stimulate a broader collaborative effort between biologists, engineers and resource managers that continues to move the state-of-the-art forward. This effort should not consider the plurality of methods or analytical procedures as competing approaches, but rather as representing a tool-rich environment upon which researchers and practitioners can draw to provide scientifically based quantifications in support of management decisions which must protect and enhance our aquatic ecosystems. © 1998 John Wiley & Sons, Ltd.