氟硅唑微乳剂的研制

对国内外文献未见报道的8％氟硅唑微乳剂制剂进行了研制，测定了其冷贮和热贮稳定性等理化性状及生物活性。对黄瓜自粉病室内毒力测定和田间药效试验结果表明，药效与40％乳油相当，对黄瓜安全。     

Inhibition and recovery of cucumber roots given multiple treatments of ferulic acid in nutrient culture

Ferulic acid, a frequently cited allelopathic agent, inhibited photosynthesis, leaf expansion, and root elongation of cucumber seedlings grown in aerated nutrient cultures in a growth chamber. Other effects were a reduction in the proportion of radioactivity fixed by photosynthesis translocated to roots, a stimulation in secondary root initiation, and an increase in root-shoot ratios. Inhibition of leaf expansion and root elongation induced by multiple ferulic acid treatments was rapidly lost once ferulic acid was removed from the root environment. The changes in general root morphology, i.e., average root length and root number, associated with ferulic acid treatments, were partially reversed or not affected when ferulic acid was removed from the root environment.Paper No. 11411 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, North Carolina 27695-7601. The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named, nor criticism of similar ones not mentioned.     

不同抗、感枯萎病黄瓜品种不同生育时期根际微生物数量消长动态分析

通过对不同抗、感枯萎病黄瓜品种不同生育时期的根际微生物数量消长动态研究发现，黄瓜抗、感枯萎病品种的根际真菌、放线菌数量存在明显差异，且这些根际微生物数量与黄瓜的生育期有一定关系。在结瓜期抗病品种的真菌、放线菌数量明显多于感病品种，黄瓜的根际细菌数量与黄瓜抗性无相关性。根际真菌、放线菌数量在黄瓜初花期、结瓜期呈明显上升趋势，而细菌数量则呈波动变化，在初花期出现下降趋势。     

黄瓜褐斑病病原菌鉴定及生物学特性研究

对辽宁省保护地发生的黄瓜褐斑病进行病原菌的分离、鉴定 ,明确该病的病原菌为多主棒孢霉[Corynesporacassiicola(B.&C.)Wei]。生物学特性研究表明 ,该菌孢子萌发的最适温度范围为15～35℃ ,最适pH值为5～8,水滴是孢子萌发的必要条件。病菌营养生长的最适温度为15～35℃ ,最适 pH值为6～8 ,在PSA、PDA等常规培养基上生长良好 ,光照对菌丝生长速度影响不显著。病菌产孢的最适温度为25～30℃,最适pH值为5～8,光照有助于孢子形成,在PSA、PDA等常规培养基上能大量产孢。病菌分生孢子在室外难以安全越冬,在保护地内能够安全越夏。该菌寄主范围广,能寄生黄瓜和番茄等20余种作物     

基于Q位移二分树复数小波变换算法的图像特征提取

为了提高图像特征的识别率,研究并设计了基于Q位移二分树复数小波变换算法的图像特征识别系统.论述了Q位移二分树复数小波变换算法的基本原理及滤波器的工作原理.在设计中保留了复数小波的优点,满足完全重构条件,同时具有良好的对称性.实验样本采用农作物黄瓜霜霉病叶部病斑图像,在Visual C 软件平台上对样本进行了训练和学习的仿真实验,仿真结果验证了系统的有效性.     

虎杖根提取物及分离组分对黄瓜白粉病的作用效果

在实验室内进行了虎杖（Polygonum cuspidatum Sieb.etZucc.）根提取物及其活性组分分离研究,并且对黄瓜白粉病进行了活性测定及温室小区试验。结果表明,无论提取率还是作用效果,乙醇较适合于做为虎杖根提取物的提取溶剂。虎杖根乙醇提取物和乙醚提取物对黄瓜白粉病的EC90值分别为537.85、35.38mg/L,而乙醚提取后残留物的活性很弱,说明乙醇提取物中具有杀菌作用的成分主要存在于乙醚提取物中。用pH梯度分离法对乙醚提取物进行分离,得到3个不同组分,其中以氢氧化钠分离得到的组分对黄瓜白粉病显示了极高的杀菌活性。在温室条件下虎杖乙醇提取物在800～1600mg/L下对黄瓜白粉病的防效相当或优于对照药剂三唑酮。     

二嗪磷在黄瓜中的残留检测及膳食风险评估

[目的]评价二嗪磷在黄瓜中的残留消解动态和膳食摄入风险.[方法]2015、2016年在湖北、浙江和河北进行规范残留试验,气相色谱法(FPD)分析样品.评价二嗪磷在黄瓜上的长期慢性膳食暴露风险和对消费者的保护水平.[结果]二嗪磷在黄瓜和土壤中的定量限分别为0.01、0.02 mg/kg,添加水平范围为0.01～3 mg/...     

几种生物农药对黄瓜霜霉病的防治效果

测定了几种生物农药对黄瓜霜霉病的田间防治效果,结果表明:10亿活孢子/g枯草芽孢杆菌WP使用浓度在(3.3￣1.25)×106cfu/mL范围内,相对防治效果达到71.78%￣82.75%,0.3%苦参碱AS使用浓度在22.5￣50.0ga.i./hm2范围内,相对防效66.83%￣77.04%,且对有益生物无害。     

Genome-Wide Identification and Characterization of the CC-NBS-LRR Gene Family in Cucumber (Cucumis sativus L.)

The NBS-LRR (NLR) gene family plays a pivotal role in regulating disease defense response in plants. Cucumber is one of the most important vegetable crops in the world, and various plant diseases, including powdery mildew (PM), cause severe losses in both cucumber productivity and quality annually. To characterize and understand the role of the CC-NBS-LRR(CNL) family of genes in disease defense response in cucumber plants, we performed bioinformatical analysis to characterize these genes systematically. We identified 33 members of the CNL gene family in cucumber plants, and they are distributed on each chromosome with chromosome 4 harboring the largest cluster of five different genes. The corresponding CNL family member varies in the number of amino acids and exons, molecular weight, theoretical isoelectric point (pI) and subcellular localization. Cis-acting element analysis of the CNL genes reveals the presence of multiple phytohormone, abiotic and biotic responsive elements in their promoters, suggesting that these genes might be responsive to plant hormones and stress. Phylogenetic and synteny analysis indicated that the CNL proteins are conserved evolutionarily in different plant species, and they can be divided into four subfamilies based on their conserved domains. MEME analysis and multiple sequence alignment showed that conserved motifs exist in the sequence of CNLs. Further DNA sequence analysis suggests that CsCNL genes might be subject to the regulation of different miRNAs upon PM infection. By mining available RNA-seq data followed by real-time quantitative PCR (qRT-PCR) analysis, we characterized expression patterns of the CNL genes, and found that those genes exhibit a temporospatial expression pattern, and their expression is also responsive to PM infection, ethylene, salicylic acid, and methyl jasmonate treatment in cucumber plants. Finally, the CNL genes targeted by miRNAs were predicted in cucumber plants. Our results in this study provided some basic information for further study of the functions of the CNL gene family in cucumber plants.