Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.     

A New Comb Circular Polarizer Suitable for Millimeter‐Band Application

This letter presents a new polarizer which has a simple comb structure inside a circular waveguide. The electrical performance of the proposed comb polarizer is optimized by a circular waveguide radius and by the physical parameters of the comb plates. This polarizer is suitable for providing good performance in millimeter‐band application because of its simple structure and low fabrication cost. In our experiments the dual‐band comb polarizer designed in band 1(K) and band 2(Ka) showed good electrical performance without any tuning elements.     

IEEE 802.11g性能分析及应用

全面介绍IEEE802.11g标准的无线局域网,详细讲述IEEE802.11g草案标准的概念、产生背景、特点、构件及其体系结构和发展前景,探讨实现IEEE802.11gWLAN所需的关键技术及其双频多模应用方式,同时分析IEEE802.11g标准的网络性能。     

Characterization and optimization of copper chemical mechanical planarization

The feature scale planarization of the copper chemical mechanical planarization (CMP) process has been characterized for two copper processes using Hitachi 430-TU/Hitachi T605 and Cabot 5001/Arch Cu10K consumables. The first process is an example of an abrasive-free polish with a high-selectivity barrier slurry, while the second is an example of a conventional abrasive slurry with a low-selectivity barrier slurry. Copper fill planarization has been characterized for structures with conformal deposition as well as with bumps resulting from bottom-up fill. Dishing and erosion were characterized for several structures after clearing. The abrasive-free polish resulted in low sensitivity to overpolish and low saturation levels for dishing and erosion. Consequently, this demonstrated superior performance when compared to the International Technology Roadmap for Semiconductors (ITRS) 2000 roadmap targets for planarization. While the conventional slurry could achieve the 0.13-μm technology node requirements, the abrasive-free polish met the planarization requirements beyond the 0.10-μm technology node.     

光纤0_2/CO_2复合传感器的研制

本文报导一种新型的光纤氧、二氧化碳复合传感器.通过在同一敏感膜载体上固定两种不同的荧光试剂——芘丁酸及羟基芘三磺酸,制作了一种对氧和二氧化碳敏感的复合敏感膜.该传感器在医学临床检验范围内具有良好的线性,其测氧的分辩率是0.1％,测二氧化碳的分辩率是0.5％,响应时间短于1min.文中还讨论了三种敏感膜载体的比较及复合传感器测量进程中氧和二氧化碳的相互干扰问题.     

Temporal Specificity of Extinction in Autoshaping.

Three experiments investigated the effects of varying the conditioned stimulus (CS) duration between training and extinction. Ring doves (Streptopelia risoria) were autoshaped on a fixed CS-unconditioned stimulus (US) interval and extinguished with CS presentations that were longer, shorter, or the same as the training duration. During a subsequent test session, the training CS duration was reintroduced. Results suggest that the cessation of responding during an extinction session is controlled by generalization of excitation between the training and extinction CSs and by the number of nonreinforced CS presentations. Transfer of extinction to the training CS is controlled by the similarity between the extinction and training CSs. Extinction learning is temporally specific. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

All‐hexahedral element meshing: automatic elimination of self‐intersecting dual lines

There has been some degree of success in all‐hexahedral meshing. Standard methods start with the object geometry defined by means of an all‐quadrilateral mesh, followed by the use of the combinatorial dual to the mesh in order to define the internal connectivities among elements. For all of the known methods using the dual concept, it is necessary to first prevent or eliminate self‐intersecting (SI) dual lines of the given quadrilateral mesh. The relevant features of SI lines are studied, giving a method to remove them, which avoids deforming the original geometry. Some examples of resulting meshes are shown where the current meshing method has been successfully applied. Copyright © 2002 John Wiley & Sons, Ltd.     

A semi-analytic boundary element method for parabolic problems

A new semi-analytic solution method is proposed for solving linear parabolic problems using the boundary element method. This method constructs a solution as an eigenfunction expansion using separation of variables. The eigenfunctions are determined using the dual reciprocity boundary element method. This separation of variables-dual reciprocity method (SOV-DRM) allows a solution to be determined without requiring either time-stepping or domain discretisation. The accuracy and computational efficiency of the SOV-DRM is found to improve as time increases. These properties make the SOV-DRM an attractive technique for solving parabolic problems.     

New quadratic models for the maximum weighted cut problem

New quadratic models are proposed to improve the upper-bound estimates in the maximum weighted cut problem. They are found by two original methods for deriving redundant quadratic constraints. A well-known linear model is shown to follow from the models proposed. Recommendations on how to develop its strengthened analogs are given. This study is partially sponsored from the grant UM2-2574-KV-03 (CRDF Cooperative Grants Program). __________ Translated from Kibernetika i Sistemnyi Analiz, No. 1, pp. 63—75, January–February 2006.     

A Detection-Theoretic Model of Echo Inhibition.

A detection-theoretic analysis of the auditory localization of dual-impulse stimuli is described, and a model for the processing of spatial cues in the echo pulse is developed. Although for over 50 years "echo suppression" has been the topic of intense theoretical and empirical study within the hearing sciences, only a rudimentary understanding of its mechanisms has emerged. In this article, psychometric functions and results from matching studies are used in developing a model that specifies the perceived position of the echo pulse as a normal deviate, with an expectation that is a logistic function of the echo delay and a variance that depends on interaural time difference. Loss of information in the echo event is quantified as a decline in the efficiency with which the binaural system receives information from the lag impulse. (PsycINFO Database Record (c) 2010 APA, all rights reserved)