基于碳量子点/羧基化石墨相氮化碳复合材料的电化学发光适体传感器检测牛奶中林可霉素

目的 构建一种高灵敏的核酸电化学发光(electrochemiluminescence,ECL)适体传感器检测牛奶中的林可霉素(lincomycin, LIN)残留。方法 以壳聚糖为交联剂,在玻碳电极(glassy carbon electrode, GCE)表面固定羧基化石墨相氮化碳(carboxylated functionalized graphite-like carbon nitride,C-g-C₃N₄)和碳量子点(carbon quantum dots,CQDs),制得CQDs/C-g-C₃N₄/GCE。电极活化后,将LIN的适配体(Apt-DNA)和二茂铁标记的DNA (Fc-DNA)修饰至电极表面,构建新型的电化学发光适体传感界面。采用循环伏安和交流阻抗对传感器的构建过程及电化学性能进行考察,同时对传感器的电化学发光行为进行分析,并应用于牛奶中LIN的检测。结果 CQDs和C-g-C₃N₄之间能产生强烈的协同效应,复合材料不仅能提供大量的...     

毛细管电泳-电化学发光分离检测辣椒粉中罗丹明B

建立一种以三联吡啶钌（Ru(bpy)32＋）为发光体系的毛细管电泳-电化学发光检测系统,并将其应用于分离和测定辣椒粉中的罗丹明B含量。考察检测电压、Ru(bpy)32＋溶液浓度、磷酸盐缓冲液的pH值和浓度、分离电压、进样电压与进样时间等因素对分离检测的影响。结果表明：在检测电压1.14 V、Ru(bpy)32＋溶液浓度5 mmol/L、磷酸盐缓冲溶液浓度20 mmol/L（pH 8.0）、进样时间10 s、进样电压10 kV、分离电压15 kV条件下,罗丹明B在6 min中得到分离。方法的线性范围为5×10－7～5×10－5 mol/L,检出限为1×10－7 mol/L（RSN=3）。对1.0×10－5 mol/L的罗丹明B标准溶液连续测定5 次,迁移时间和峰面积的相对标准偏差分别为3.2%和1.5%。该方法可成功应用于辣椒粉中罗丹明B含量的测定。     

A Facile Microwave Avenue to Electrochemiluminescent Two‐Color Graphene Quantum Dots

With the assistance of microwave irradiation, greenish‐yellow luminescent graphene quantum dots (gGQDs) with a quantum yield (QY) up to 11.7% are successfully prepared via cleaving graphene oxide (GO) under acid conditions. The cleaving and reduction processes are accomplished simultaneously using microwave treatment without additional reducing agent. When the gGQDs are further reduced with NaBH₄, bright blue luminescent graphene quantum dots (bGQDs) are obtained with a QY as high as 22.9%. Both GQDs show well‐known excitation‐dependent PL behavior, which could be ascribed to the transition from the lowest unoccupied molecular orbital (LUMO) to the highest occupied molecular orbital (HOMO) with a carbene‐like triplet ground state. Electrochemiluminescence (ECL) is observed from the graphene quantum dots for the first time, suggesting promising applications in ECL biosensing and imaging. The ECL mechanism is investigated in detail. Furthermore, a novel sensor for Cd²⁺ is proposed based on Cd²⁺ induced ECL quenching with cysteine (Cys) as the masking agent.     

Spectral analysis in ultraweak emissions of chemi- and electrochemiluminescence systems

Investigation of ultraweak emissions in the processes of chemiluminescence, CL, and electrochemiluminesce, ECL, requires special techniques of their recording and spectral analysis. From among the hitherto proposed methods of detection of the emission spectra of these processes, that of the cut-off filter was most sensitive. The usefulness of this method in interpretation of the CL and ECL systems of the quantum yields in the range 1×10⁻⁹−1×10⁻¹¹ containing ions and complexes of Eu(III), Tb(III) and Dy(III) was shown. Exceptional character of the emission bands of lanthanide ions, being a result of the f-f electron transitions and in particular their low FWHM, permitted the application of the cut-off filter method to their analysis. The results obtained for CL and ECL on the basis of analysis of ultraweak emission proved to be successful in analytical applications. The systems containing Eu(III) ions hydrated or complexed with organic ligands enabled inferring changes in the coordination sphere of the ions.     

Non-uniform current density distribution at a rde. Observation by means of photographing electrochemiluminescence

Equations for the current at a rde have been derived with the presumption that equal accessibility for any place on the electrode surface. An example of non-homogeneous current density distribution at the Pt-rde was observed in photographs of electrogenerated chemiluminescence (ECL) where the intensity of emission changes with the distance from the centre and depends on the type of the process and on the rotation velocity.     

化学发光酶免疫分析与电化学发光免疫分析检测 CA19-9结果的一致性比较

目的：探讨化学发光酶免疫分析（CLEIA）与电化学发光免疫分析系统分析（ECLIA）检测糖类抗原19-9（CA19-9）结果的可比性,保证检验结果的一致性。方法分别在罗氏 Cobas e601型全自动电化学发光免疫分析仪和国产的郑州安图 Lumo 化学发光免疫分析仪平行测定 CA19-9,然后对检测结果进行统计学处理和分析。结果两检测系统精密度高,检测结果差距无统计学意义（P ＞0．05）,2组数据存在良好的线性关系（r 2＝0．9975＞0．99）,预期偏倚和相对偏倚均可接受。结论CLEIA 与 ECLIA 检测 CA19-9的结果相关性良好,有较好的可比性,结果基本一致。     

Light‐Emitting Devices Based on Electrochemiluminescence Gels

Electrochemiluminescence (ECL) is a self‐emission of light from electrochemically excited luminophores via a series of redox reactions. Over the past decade, light‐emitting devices based on gel‐phase ECL active materials, i.e., gel electrolyte composites (referred to as ECL gels) containing an ECL luminophore, electrolyte, and network matrix, have attracted considerable attention as a complementary device platform to conventional electroluminescent devices for low‐cost printable displays and solid‐state light sources. Although the ECL phenomenon is extensively exploited in analytical diagnostics and sensing, the development of printable and fast‐response gel‐type luminescent materials may further expand the potential application of ECL in solid‐state flexible, bendable, and stretchable light‐emitting devices. This review summarizes the operation mechanisms of ECL‐based light‐emitting devices, ECL emitters and electrolytes, engineering strategies for obtaining printable high‐strength/high‐conductivity ECL gels, and emerging applications of gel‐type ECL devices.     

光学生物传感器的研究进展

生物传感器是一种在多学科交叉背景下发展起来的新型测试装置,其中光学生物传感器因具有快速、灵敏、准确和高选择性等特点而最为引人瞩目.综述了光学生物传感器的原理、结构和分类;对比讨论了各类光学生物传感器的特性、应用领域、存在的主要问题及其今后的发展趋势;评述了以新型无机材料作为标记物的光致荧光生物传感器的研究意义以及发展前景.     

Detection of ricin contamination in liquid egg by electrochemiluminescence immunosorbent assay

A monoclonal antibody-based electrochemical luminescence method was developed for detecting and quantifying ricin in liquid egg, with a limit of detection of 0.2 ng/mL. Because this highly toxic protein, present in the seeds of Ricinus communis (castor), has been used for intentional poisoning in the past, it is important to have sensitive and reliable analytical methodology to detect ricin in food matrices such as liquid egg. The detection of this quantity of pure or crude ricin spiked into commercial samples of liquid egg provides approximately 50000-fold greater sensitivity than required to detect a toxic dose of ricin (>1 mg) in a 100 g sample. PRACTICAL APPLICATION: Because ricin has been used for intentional poisoning, there is a need for analytical methodology to detect ricin in food matrices to assure a safe food supply. Using monoclonal antibodies to ricin developed in our laboratory, we explored an assay readout system known as electrochemiluminescence. This technique afforded sensitive and specific analysis of ricin intentionally added to liquid egg and could potentially be used to monitor egg-based vaccine production.     

偕胺肟基团功能化共轭介孔聚合物用于可监控快速海水提铀

海水提铀是核能可持续发展的重要保障之一。快速提铀材料凭借短吸附周期可减少生物污损、老化而延长使用寿命,因此优化吸附周期可获得更高的提铀效率和经济效益。为此,本研究设计了一种偕胺肟修饰的共轭介孔聚合物（CMPAO）用于可监控的快速海水提铀。以具有优良聚集诱导发光性质的三苯胺和修饰有偕胺肟的芴衍生物为构建单元合成吸附材料,UO2+2被偕胺肟基团捕捉后,经共振能量转移增强CMPAO的电化学发光（ECL）信号,从而实现吸附过程的监测,以确定最优的吸附周期。结果表明：CMPAO在铀酰溶液（5×10-5 mol/L）中可在20 min内达到吸附平衡,对U的吸附容量为1825 mg/g;在真实海水中,3 d即可基本完成吸附过程,CMPAO对U的吸附容量达到16 mg/g,同时CMPAO在海水中可实现铀吸附量与ECL强度的正相关,能够实时监测吸附过程,指示吸附量的变化,以确定最优吸附周期。