A method for quantifying cell size from differential interference contrast images: validation and application to osmotically stressed chondrocytes

An automatic image analysis method was developed to determine the shape and size of spheroidal cells from a time series of differential interference contrast (DIC) images. The program incorporates an edge detection algorithm and dynamic programming for edge linking. To assess the accuracy and working range of the method, results from DIC images of different focal planes and resolutions were compared to confocal images in which the cell membrane was fluorescently labelled. The results indicate that a 1‐µm focal drift from the in‐focus plane can lead to an overestimation of cell volume up to 14.1%, mostly due to shadowing effects of DIC microscopy. DIC images allow for accurate measurements when the focal plane lies in a zone slightly above the centre of a spherical cell. In this range the method performs with 1.9% overall volume error without taking into account the error introduced by the representation of the cell as a sphere. As a test case, the method was applied to quantify volume changes due to acute changes of osmotic stress.     

Na+ and/or Cl− Toxicities Determine Salt Sensitivity in Soybean (Glycine max (L.) Merr.), Mungbean (Vigna radiata (L.) R. Wilczek), Cowpea (Vigna unguiculata (L.) Walp.), and Common Bean (Phaseolus vulgaris L.)

Grain legumes are important crops, but they are salt sensitive. This research dissected the responses of four (sub)tropical grain legumes to ionic components (Na⁺ and/or Cl⁻) of salt stress. Soybean, mungbean, cowpea, and common bean were subjected to NaCl, Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and a “high cation” negative control for 57 days. Growth, leaf gas exchange, and tissue ion concentrations were assessed at different growing stages. For soybean, NaCl and Na⁺ salts impaired seed dry mass (30% of control), more so than Cl⁻ salts (60% of control). All treatments impaired mungbean growth, with NaCl and Cl⁻ salt treatments affecting seed dry mass the most (2% of control). For cowpea, NaCl had the greatest adverse impact on seed dry mass (20% of control), while Na⁺ salts and Cl⁻ salts had similar intermediate effects (~45% of control). For common bean, NaCl had the greatest adverse effect on seed dry mass (4% of control), while Na⁺ salts and Cl⁻ salts impaired seed dry mass to a lesser extent (~45% of control). NaCl and Na⁺ salts (without Cl⁻) affected the photosynthesis (P_n) of soybean more than Cl⁻ salts (without Na⁺) (50% of control), while the reverse was true for mungbean. Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and NaCl had similar adverse effects on P_n of cowpea and common bean (~70% of control). In conclusion, salt sensitivity is predominantly determined by Na⁺ toxicity in soybean, Cl⁻ toxicity in mungbean, and both Na⁺ and Cl⁻ toxicity in cowpea and common bean.     

A New Method for Identifying Osmotically Limited and Gel Layer Controlled Pressure Independent Flux in Ultrafiltration

An unequivocal determination of whether pressure independent flux regime is osmotically controlled or gel layer dominated, is still open for discussion in the membrane literature. The present work reports a method that could be used to address this issue. It is shown that analysis of post steady state transient filtration data leads to clear demarcation of osmotically limited and gel layer controlled filtration. The method proposed in this work can also be used to estimate the additional filtration resistance offered by the polarization layer to the permeate flow in macromolecular ultrafiltration and has been verified experimentally. It has also been shown that the polarization layer thickness is not sensitive to the feed pressure but varies as a function of the bulk solute concentration; higher the bulk concentration, thicker is the polarization layer.     

钠基膨润土防水毯在滹沱河防洪整治中的试验研究

结合滹沱河防洪综合整治工程,对钠基膨润土防水毯建立试验模型,着重研究不同试验水头、外界环境条件(温度、风力等因素)对钠基膨润土防水毯的渗水量、水分蒸发量和渗透系数的变化规律。研究结果表明:试验水头与渗透系数成非线性关系,且渗水量随水位的增加而增加,渗透系数随水位的增加而减小。通过模型试验验证了钠基膨润土防水毯高密实、高防水等特点,为类似的防洪综合整治工程提供了技术支撑。     

海盐水溶液渗透压计算的探讨

在反渗透脱盐装置的设计中,渗透压是必不可少的设计参数。考虑在水处理过程中遇到的混合电解质水溶液绝大多数的离子强度都不高,据此作者提出一近似计算公式。在盐溶液浓度为1000～50000 ppm 范围内,用该式计算所得渗透压值与实测值及Stoughton 式计算值相比,相对误差在2.5%以内。     

渗透蒸馏的传质过程及应用

采用聚丙烯微孔滤膜，研究了渗透蒸馏的质量传递机理。改变盐浓度、盐的种类及操作条件，结果表明，纯水渗透通量与盐溶液浓度成线性关系，但不同种类的盐具有不同的渗透蒸馏性能。本文还用该技术对黑米液进行浓缩处理，浓缩液比重能上升到１．２ｇ／ｃｍ＾３，完全能满足浓缩要求，只是膜的使用学有待进一步提高。     

Effects of different osmo‐dehydrofreezing treatments on the volatile compounds,phenolic compounds and physicochemical properties in mango (Mangifera indica L.)

The influences of different osmotic solution pretreatments (sucrose, glucose and maltose with concentration of 45%) on the volatile compounds, phenolic compounds and other physicochemical parameters in frozen mangoes were studied. Volatile and phenolic compounds were measured using SPME/GC‐MS and HPLC method, respectively. The results revealed that compared with untreated frozen mango, some volatile compounds (e.g. alcohols, aldehydes and some terpenes and esters) increased significantly and there were more compounds identified in osmo‐dehydrofrozen samples. These changes positively affected mango aroma and resulted in higher sensory scores for osmo‐dehydrofrozen samples. In addition, data on phenolic compounds showed that dehydrofrozen samples pretreated in glucose and sucrose had higher ρ‐hydroxybenzoic acid and quercetin contents, respectively, while samples pretreated in maltose showed higher ρ‐coumaric acid and sinapic acid contents compared with untreated frozen samples. The current work indicates that osmo‐dehydrofreezing can improve the retention of phenolic and aromatic compounds in frozen mango.     

On the feasibility of ocean brine pool power stations

The demonstrated existence of vast brine pools in a number of places in the ocean basin around the world offers an interesting opportunity to the production of power from ocean, which has not been yet considered. The attractiveness of these vast deposits of brine lie in the fact that can be readily dissolved with the surrounding top waters extracting the spontaneous osmotic mixing energy released. In addition, osmotic engines can pumped out the brackish streams by buoyancy without the add of pressure exchangers (PEXs) as is required in current pressure retarded osmosis (PRO) technology and then simplifying significatively the overall process. Utilizing a simplified physical model, a first estimation for the density of power per unit of membrane area was calculated.