牙鲆淋巴囊肿病的PCR诊断方法研究

以中国养殖牙鲆 (Paralichthysolivaceus)淋巴囊肿病毒 (Lymphocystisdiseasevirus,LCDVcn)主要衣壳蛋白 (majorcapsidprotein ,MCP)基因的中间保守序列为目标基因 ,设计了一对特异性引物。该引物可扩增出 172bp的病毒DNA片段 ,其最小DNA检出量为 0 0 183ng。用PCR法从人工感染淋巴囊肿病毒 3天的牙鲆血、鳃、肝、脾、肠、胃及自然发病牙鲆的肿瘤中 ,分别检测到了LCDV的存在。本实验结果证明 ,PCR法对于早期检测LCDV是十分有效的。     

Prevention of musculoskeletal disorders at work: Validation and reliability in a multicenter intervention study

During the years 1986–1989 The Finnish Work Environment Fund initiated a multicenter, intervention study (programme) to prevent musculoskeletal disorders at work. The programme consisted of four different projects comprising methodological, epidemiological, and interventive themes. The main results were as follows: (1) More than half of the occupational physiotherapists and physicians involved in the project felt that they did not have adequate training for testing the performance capacity of the musculoskeletal system. Several of the most commonly used musculoskeletal tests had either poor validity in predicting future musculoskeletal troubles or had poor reliability. (2) A controlled intervention study showed that active training of neck-shoulder muscles can prevent musculoskeletal troubles, even more than ergonomic actions. (3) It is possible to reduce sick-leave due to low-back disorders by intervention measures directed toward both the work (environment, tools) and the workers (working methods, positive attitude to work, preparedness to keep fit). (4) The identification of ergonomic hazards with the help of teamwork proved to be feasible. The practical improvements, however, were difficult to implement in the prevailing conditions. Some of the improvements increased the work load and the stress of the workers. This aspect needs further consideration.     

新一代天气雷达发射机故障诊断分析

我省的新一代天气雷达在多年的运行过程中出现了各种不同类型的故障,有些严重故障直接导致雷达停机,影响了气象预报服务.本文介绍和总结了该雷达发射机部分出现的一些主要故障,这些故障都集中在雷达射频驱动器、聚焦线圈电源、触发器、调制器、后充电调整器等组件上.通过对这些组件的功能和工作原理的分析,详细介绍发射机故障诊断分析的关键技术.     

MALDI-TOF MS: an upcoming tool for rapid detection of antibiotic resistance in microorganisms

MALDI-TOF MS profiling for microorganism detection has already been demonstrated in the 1990s, but has evolved to the first-line identification method in many laboratories just during the past five years. While this application of MALDI-TOF MS has proven its broad applicability, accuracy, robustness, and cost-effectiveness it is of particular interest to expand the capabilities of the mass spectrometric platform. Resistance detection is the most desirable further application of MALDI-TOF MS in microbiology, but maybe also the most challenging. Different approaches have been published regarding diverse antibiotic drugs and distinct microorganism classes. The current review shall give an overview about the developments of the recent years and their potential to get transformed in clinical useful assays in the future.     

Compact optical diagnostic device for isothermal nucleic acids amplification

We recently reported the successful use of the loop-mediated isothermal amplification (LAMP) reaction for hepatitis B virus (HBV) DNA amplification and its optimal primer design method. In this study, we report the development of an integrated isothermal device for both amplification and detection of targeted HBV DNA. It has two major components, a disposable polymethyl methacrylate (PMMA) micro-reactor and a temperature-regulated optical detection unit (base apparatus) for real-time monitoring of the turbidity changes due to the precipitation of DNA amplification by-product, magnesium pyrophosphate. We have established a correlation curve (R² = 0.99) between the concentration of pyrophosphate ions and the level of turbidity by using a simulated chemical reaction to evaluate the characteristics of our device. For the applications of rapid pathogens detection, we also have established a standard curve (R² = 0.96) by using LAMP reaction with a standard template in our device. Moreover, we also have successfully used the device on seven clinical serum specimens where HBV DNA levels have been confirmed by real-time PCR. The result indicates that different amounts of HBV DNA can be successfully detected by using this device within 1 h.     

Local influence in estimating equations

Local influence diagnostics based on estimating equations as the role of a gradient vector derived from any fit function are developed for repeated measures regression analysis. Our proposal generalizes tools used in other studies ( [Cook, 1986] and [Cadigan and Farrell, 2002]), considering herein local influence diagnostics for a statistical model where estimation involves an estimating equation in which all observations are not necessarily independent of each other. Moreover, the measures of local influence are illustrated with some simulated data sets to assess influential observations. Applications using real data are presented.     

Feature-based assessment of visuo-spatial neglect patients using hand-drawing tasks

Visuo-spatial neglect (VSN) is a post-stroke condition in which a patient fails to respond to stimuli on one side of the visual field. Using an established pencil-and-paper-based method for the assessment of VSN (the Rivermead Behavioural Inattention Test) as a reference, a battery of computer-based hand-drawing tests is developed and shown to be effective in distinguishing between stroke subjects with and without neglect. The novel approach adopts measurements both of the outcome and the process by which the drawing tasks are executed. This approach provides a novel diagnostic capability which results in increased test sensitivity, a more objective assessment and a reduction in overall evaluation time. The paper describes the development of a binary assessment system using the computer-based acquisition and analysis of task data alongside feature selection techniques to maximise performance.

基于C/S+B/S双模式的分布式远程诊断专家系统

分布式远程诊断专家系统（DRFDES）是故障诊断领域的发展趋势之一。为此文章深入研究了其工作原理、实现的硬软件结构及工作过程，分析了Web数据库的体系的两层C／S结构和三层B／S结构厦其在DRFDES中的优缺点，在该基础上提出了一种基于C／S和B／S混合模式的数据库体系的分布式远程故障诊断专家系统，探讨了故障诊断知识库的更新模式，并对分布式远程诊断专家系统实现的理论基础和关键技术进行了研究。     

轴承试验中心分布式监控管理系统

本文设计了轴承试验中心分布式监控管理系统，系统可对多台试验机进行在线监测，对被试验轴承做实时诊断，有故障时立即报警，并可将整个试验过程数据通过网络传送到上位机进行显示并存储到数据库中，供技术人员分析使用。该系统已在轴承生产企业得到了成功的应用。     

Artificial intelligence implementation in the APS process diagnostic

Thermal spray process is a technique of coating manufacturing implementing a wide variety of materials and processes. This technique is characterized by up to 150 processing parameters influencing the coating properties. The control of the coating quality is needed through the consideration of a robust methodology that takes into account the parameter interdependencies, the process variability and offers the ability to quantify the processing parameter-process response relationships. The aim of this work is to introduce a new approach based on artificial intelligence responding to these requirements. A detailed procedure is presented considering an artificial neural network (ANN) structure which encodes implicitly the physical phenomena governing the process. The implementation of such a structure was coupled to experimental results of an optic sensor controlling the powder particle fusion state before the coating formation. The optimization steps were discussed and the predicted results were compared to the experimental ones allowing the identification of the control factors.