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排序方式: 共有480条查询结果,搜索用时 250 毫秒
1.
Emna Ouni Sbastien Pyr dit Ruys Marie-Madeleine Dolmans Gaëtan Herinckx Didier Vertommen Christiani A. Amorim 《International journal of molecular sciences》2020,21(23)
Currently, the extracellular matrix (ECM) is considered a pivotal complex meshwork of macromolecules playing a plethora of biomolecular functions in health and disease beyond its commonly known mechanical role. Only by unraveling its composition can we leverage related tissue engineering and pharmacological efforts. Nevertheless, its unbiased proteomic identification still encounters some limitations mainly due to partial ECM enrichment by precipitation, sequential fractionation using unfriendly-mass spectrometry (MS) detergents, and resuspension with harsh reagents that need to be entirely removed prior to analysis. These methods can be technically challenging and labor-intensive, which affects the reproducibility of ECM identification and induces protein loss. Here, we present a simple new method applicable to tissue fragments of 10 mg and more. The technique has been validated on human ovarian tissue and involves a standardized procedure for sample processing with an MS-compatible detergent and combined centrifugation. This two-step protocol eliminates the need for laborious sample clarification and divides our samples into 2 fractions, soluble and insoluble, successively enriched with matrisome-associated (ECM-interacting) and core matrisome (structural ECM) proteins. 相似文献
2.
DVB条件接收系统中独立加扰器的设计与实现 总被引:2,自引:0,他引:2
本文介绍DVB条件接收系统的基本结构,详细分析了条件接收系统的工作原理,并给出条件接收系统中独立加扰器SAS(Stand Alone Scrambler)的具体实现方案。 相似文献
3.
Yaxin Tang 《Materials and Manufacturing Processes》2002,17(6):789-796
An experimental apparatus was designed and built for the hybrid process of laser enhanced electrochemical machining (ECM). Comparison experiments had been done between solely ECM and laser enhanced ECM. The machining performance of hybrid process was investigated and compared with solely ECM. The principle of the hybrid process was exposed by analyzing the microstructure of machining surface. The results show that the hybrid process has advantages in machining accuracy, surface quality, and machining efficiency. 相似文献
4.
基于Ansys的电化学加工辅助阴极设计 总被引:3,自引:0,他引:3
根据电化学加工极间间隙电势分布的拉普拉斯方程,提出了一种阴极设计算法-误差调整法。该算法的实现主要采用了Ansys有限元计算软件。计算结果表明:该法计算迭代次数较为适当,对于精度要求不高的电化学加工阴极设计,基于Ansys的辅助阴极设计有效可行。 相似文献
5.
可重构电解加工机床研究 总被引:1,自引:0,他引:1
电解加工机床通常是根据用户具体需求单机生产,机床研制周期长、设计重复、成本高。为此,提出采用开放式控制系统、模块化思想开发可重构电解机床,并对机床的机械部分、控制系统及通信系统进行了研究。 相似文献
6.
从电子对抗力量体系的构建原则入手,结合电子对抗作战特点,对运用电子对抗力量与其他作战力量协同作战问题进行了探讨,就提高电子对抗力量协同作战能力提出了一些基本措施. 相似文献
7.
传统以平台为中心的电子对抗作战方式是点对点的对抗,无法满足体系对抗的需要,以网络为中心的空军电子对抗作战运用在情报共享的基础上,合理、高效调用作战资源,使得作战方式更加灵活,作战效能更加突出.基于网络中心战的基本概念,探讨了基于网络中心战的机载电子对抗作战方式,由此得到了几点启示. 相似文献
8.
To generate the false targets in the desired area accurately, a novel two dimensional(2-D) intermittent sampling jamming algorithm against synthetic aperture radar(SAR) is presented. The intercepted radar signals are processed via 2-D intermittent sampling and Doppler shift-frequency and retransmitted to radar. Hence, the false target-lattice emerges in the specified area. The generation mechanism, position and distribution characteristics of false target-lattice are discussed. Meanwhile, the reason for the asymmetric amplitude of the target false-lattice and the modulation factor of energy compensation are presented. The correctness of theoretical analysis and validity of the proposed algorithm are verified through simulation experiments. Compared with conventional methods, the critical requirement of the jammer position can be reduced by the proposed method. 相似文献
9.
10.
Development of new-concept desk top size machine tool 总被引:6,自引:0,他引:6
A desktop multiprocess machinery has been designed that has two concepts: miniaturizing of machine tool and multiprocessing with a same machine tool. The prototype of desktop multiprocess machinery is developed in this study. This is tabletop size machine tool that has five changeable machining heads. Outline of main body and machining head are presented.In order to know the basic accuracy of desktop multiprocess machinery, experimental evaluation is carried out. Machining head setting error, stiffness of multiprocess machinery and straightness of X, Y, Z stage is measured. To study the basic performance of desktop multiprocess machinery, a complex machining experiment is carried out with the developed machine tool. The complex machining consists of three steps: electrode machining by milling, hole shaping by EDM, and hole finishing by ECM. These steps are performed in sequence on the same machine tool. The complex machining is successfully carried out. In order to evaluate the desktop multiprocess machinery from environmental point of view, machining energy, volume of machining liquid, and installation space of desktop multiprocess machinery are measured. The measured values are compared with estimated values with conventional machine tools. The machining energy, the volume of machining liquid, and the installation space of desktop multiprocess machinery are smaller than those of conventional machine tool. 相似文献