Omics Analysis of Blood-Responsive Regulon in Bordetella pertussis Identifies a Novel Essential T3SS Substrate

Bacterial pathogens sense specific cues associated with different host niches and integrate these signals to appropriately adjust the global gene expression. Bordetella pertussis is a Gram-negative, strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Though B. pertussis does not cause invasive infections, previous results indicated that this reemerging pathogen responds to blood exposure. Here, omics RNA-seq and LC–MS/MS techniques were applied to determine the blood-responsive regulon of B. pertussis. These analyses revealed that direct contact with blood rewired global gene expression profiles in B. pertussis as the expression of almost 20% of all genes was significantly modulated. However, upon loss of contact with blood, the majority of blood-specific effects vanished, with the exception of several genes encoding the T3SS-secreted substrates. For the first time, the T3SS regulator BtrA was identified in culture supernatants of B. pertussis. Furthermore, proteomic analysis identified BP2259 protein as a novel secreted T3SS substrate, which is required for T3SS functionality. Collectively, presented data indicate that contact with blood represents an important cue for B. pertussis cells.     

2Mbit/s高速信令链路在移动通信七号信令网中的应用

通过对2Mbit/s高速信令链路原理、特点的介绍，阐明了该技术在现阶段使用的实际意义，并给出了2Mbit/s高速信令链路在移动通信七号信令网中的应用。     

Corrosion Behavior of 316LN and 316 Stainless Steels During Long-term Exposure to Aerated 0.5 M NaCl Using Electrochemical Noise Technique

In the present work 316LN as well as 316 stainless steel (SS) coupons each of dimensions (0.025 × 0.018 × 0.006 m³) in well-polished condition were used as two nominal electrodes which were immersed in the aerated solution of 0.5 M NaCl. Correlated current and potential electrochemical noise (EN) signals were collected at 1 Hz sampling frequency for 1 h daily over a period of 30 days. The detrended EN data were used to calculate the noise resistance (R _N) as well as the spectral noise resistance at zero frequency (R _SN^o) values and other statistical parameters. To study the nature of pits and distribution of their diameters as well as depths, extensive observations of the pitted and the blank specimens were carried out using Confocal Laser Scanning Microscopy (CLSM). The current and the potential records of the two alloys showed distinct differences in their corrosion behavior. It was observed that within less than 4 h of immersion, 316SS showed signals indicative of unstable pitting and onset of stable pitting was noticed after 48 h of exposure. However, until about 24 h, 316LN showed just the random signals and unstable pitting was observed after 28 h. The signals clearly indicated continuous growth of the stable pits in 316SS as against the repassivation of the unstable pits in 316LN after 7 days exposure. It was observed that R _N values showed a continuous decrease in the case of 316SS, but were quite stable in the case of 316LN over the exposure period. Concurrent to these observations it was observed that 316SS specimen was extensively pitted. The frequency distributions of pit diameters as well pit depths were observed to be highest at 10-20 μm and 5-10 μm, respectively. However, pits with as large as 70-80 μm diameter and as deep as 20-25 μm too were observed. No pits were observed in case of 316LN even after 30 days of exposure, an observation that corroborates well with the stable R _N values. Thus, in the present investigation, the long-term tests using EN technique coupled with CLSM studies conclusively prove that many unstable pits initiated in 316SS turn into stable ones resulting in insidious localized corrosion attack whereas the unstable pits initiated in 316LN get passivated in the simulated coastal environment.     

薄板坯连铸生产SS400钢工艺技术研究

对唐钢薄板坯连铸机生产的SS400钢的生产工艺进行了分析,认为薄板坯连铸机生产的SS400钢,在采取了精炼站在线定氧,将钢水全氧控制在25×10~(-6)左右,同时连铸采取保护浇注和轻压下等技术后,能够确保薄板坯连铸SS400钢的顺利浇注,轧制的板带材表面质量良好,机械性能符合要求。     

龙滩工程按正常蓄水位400m方案一次建成效益巨大

2003年11月龙滩工程将实现大江截流，随着工程建设的全面推进，越发显出实施龙滩水电站正常蓄水位400m一次建成方案的重要性和紧迫性。相对分期建设而言，400m水位一次建成，工程效益大、增加投资少、无后期加高过程中的各种技术难题，基本不影响电站的初期发电效益，水轮机设计与运行问题少、机组运行条件好，电站规模效益与社会综合效益巨大，有利于移民安置和库区经济的发展，对国家、地方、企业均十分有利，因此应尽快决策实施400m一次建成方案。     

直接序列扩展频谱中伪码优选研究

本文从最优化理论和工程实际出发，全面分析和完善了直接序列扩展频谱中伪码的数学模型，在此基础上，建立了一种较准确的伪码优选数学模型，并探讨了合适的搜索算法。     

分组交换网上的增值业务

本文介绍了公用和专用分组交换数据网的现状后，说明了开展标准的增值业务系统的重要性。论述了典型的增值业务系统－电子邮件和传真存储转发系统。     

HRB400月牙型螺纹钢筋试制

本文详细介绍了包钢采用平炉冶炼工艺试制HRB400钢筋的生产过程和检验结果，对试制中出现的问题提出了改进措施，并初步研究了稀土对HRB400钢筋质量与性能的影响。结果表明，采用平炉冶炼工艺可生产出符合GBl499—1988的HRB400钢筋。本次试验研究尚未看出稀土对HRB400钢筋的质量与性能产生明显影响。     

西门子PLC在合金钢棒材连轧机上的应用

介绍基于西门子S7-400PLC的控制系统,通过Ethernet、Profibus-DP、MPI网完成系统数据的采集、交换与监控,满足合金钢棒材轧制线高动态品质、高调速精度的要求。