Evaluation of Digitally Encoded Layer‐by‐layer Coated Microparticles as Cell Carriers

To obtain more biologically relevant data there is a growing interest in the use of living cells for assaying the biological activity of unknown chemical compounds. Density ‘multiplex’ cell‐based assays, where different cell types are mixed in one well and simultaneously investigated upon exposure to a certain compound are beginning to emerge. To be able to identify the cells they should be attached to microscopic carriers that are encoded. This paper investigates how digitally encoded microparticles can be loaded with cells while keeping the digital code in the microcarriers readable. It turns out that coating the surface of the encoded microcarriers with polyelectrolytes using the layer‐by‐layer (LbL) approach provides the microcarriers with a ‘highly functional’ surface. The polyelectrolyte layer allows the growth of the cells, allows the orientation of the cell loaded microcarriers in a magnetic field, and does not hamper the reading of the code. It has further been shown that the cells growing on the polyelectrolyte layer can become transduced by adenoviral particles hosted by the polyelectrolyte layer. It is concluded that the digitally encoded microparticles are promising materials for use in biomedical and pharmaceutical in‐vitro research where cells are used as tools.     

An analysis of contemporary printed wiring board manufacturing environment in the USA

This article presents a comparative analysis of contemporary printed wiring assembly (PWA) batch manufacturing systems employing surface mount technology (SMT), This analysis is accomplished by reviewing a typical case study of PWA manufacturing system in the USA. A previously developed model is then applied to the system to predict the cost and performance in the production of typical PWA designs. Various modifications to the system are then proposed and evaluated. From the case study, conclusions concerning optimum manufacturing system configuration for this industry can be drawn and are presented.     

《计算机组装及维护》课程项目教学法研究

项目教学法对于中高职院校培养学生的实际操作能力十分有效,针对《计算机组装与维护》这门课程,通过项目设置、任务分解、组织完成的形式,激发学生学习的兴趣,提高了学生的实际动手能力,取得较好的教学效果。     

Simultaneous solving of balancing and sequencing problems with station-dependent assembly times for mixed-model assembly lines

In spite of many studies, investigating balancing and sequencing problems in Mixed-Model Assembly Line (MMAL) individually, this paper solves them simultaneously aiming to minimize total utility work. A new Mixed-Integer Linear Programming (MILP) model is developed to provide the exact solution of the problem with station-dependent assembly times. Because of NP-hardness, a Simulated Annealing (SA) is applied and compared to the Co-evolutionary Genetic Algorithm (Co-GA) from the literature. To strengthen the search process, two main hypotheses, namely simultaneous search and feasible search, are developed contrasting Co-GA. Various parameters of SA are reviewed to calibrate the algorithm by means of Taguchi design of experiments. Numerical results statistically show the efficiency and effectiveness of the proposed SA in terms of both the quality of solution and the time of achieving the best solution. Finally, the contribution of each hypothesis in this superiority is analyzed.     

浅谈出口巴基斯坦铁路客车新型酚醛玻璃钢风道的研制开发

本文阐述了新型酚醛玻璃钢风道的成型工艺、开发过程，并与传统玻璃钢风道作了比较，论述了新型酚醛玻璃钢风道的优点，并对其应用前景和对铁路客车的设计思路的影响进行了探讨。     

层次约束结构的虚拟装配建模技术

为了解决虚拟环境下装配建模在信息完备性和模型复杂性等方面的问题,提出了一种基于层次约束结构的虚拟装配模型.该模型分为产品层、部件层、零件层、特征层、几何面层和面片层来表达,不同层元素之间存在层次映射关系,同一层元素之间存在约束关系.通过CAD系统和虚拟装配系统之间的数据转换,实现了虚拟装配模型的生成算法,并给出了根据虚拟装配模型生成的虚拟装配场景图的结构.基于层次约束结构的虚拟装配模型具有信息表达完备,信息集成度高,满足实时性要求等优点,同时还能与CAD系统和虚拟装配场景图实现集成.实例验证了虚拟装配建模的有效性.