聚乙烯系新型塑料给水管的发展

介绍了聚乙烯(PE)管、交联聚乙烯(PEX)管、耐高温非交联PE管、超高摩尔质量PE管、铝塑复合管5种聚乙烯系新型塑料给水管的性能特点及其发展。     

嵌入式Linux路由器中同步PPP的实现方案

分析了在做入式Linux路由器上实现同步PPP的问题并设计了几种解决方案。通过对各种方案的对比确定了一种最优方案，并将其实现。实验结果表明，该方案可以有效地进行同步PPP的PAP和CHAP认证以及NCP协商，并能够达到同步串口速率为2Mbps。     

Specificity of a novel TaqMan PCR method for detection of poultry DNA

After the Bovine Spongiform Encephalopathy (BSE) crisis emerged in 1985/1986, all processed animal proteins (PAPs) were finally banned for use in animal feed in the European Union. To partially lift this feed ban, paths for re-introduction of PAPs from species other than ruminants e.g. pig and poultry, are described in the Transmissible Spongiform Encephalopathies (TSE) Roadmap 2. Cannibalism, however, is still not allowed. Specific detection methods for pig and poultry meal and PAPs are prerequisites for reintroduction of pig and poultry processed animal proteins into animal feed. Developing a sensitive PCR method that specifically detects the taxonomically diverse and therefore artificial group ‘poultry’ and that does not detect other birds at the same time is a challenge. Here, a novel TaqMan PCR method for poultry detection is presented. The specificity of the poultry method against target and non-target species has been extensively investigated. The efficiency, linearity and sensitivity was tested using dilution series of chicken, turkey, duck and goose DNA isolated from meat and autoclaved meat as a model system for PAPs.     

PPP协议在GPRS远程监控系统中的应用

通过对PPP进行深入研究，分析了基于GPRS的PPP协商过程，叙述了PPP组成、内容、含义和连接的过程等；并分析了PPP协商过程的数据交换情况。从而为进一步利用PPP协议实现无线远程监控提供了良好的基础。     

人小肠粘膜免疫酶的定位研究—光镜和电镜免疫组织化学染色法

本文对免疫酶组织化学的样品制备程序和染色方法做了详细的阐述。用直接法、间接法和ABC法,对人小肠免疫酶的定位,进行了光镜和电镜的观察,染色阳性反应显著,获得了满意的效果。并对染色技巧做了分析和探讨。     

血清PSA及PAP测定的临床应用评价

报道了２３例前列腺癌、４２例良性前列腺增生及２１例男性非前列腺疾病住院患者血清ＰＳＡ及ＰＡＰ联合测定结果。前列腺癌组与良性前列腺增生及对照组比较有极显著差异，按决策矩阵法对血清ＰＳＡ及ＰＡＰ进行临床综合评价，两者诊断指数和可用度分别为１３５．８％、１３８。３％和０．３６、０．４５。结果表明血清ＰＳＡ及ＰＡＰ测定对于前列腺癌诊断及鉴别诊断为互补关系，血清ＰＳＡ测定作为前列腺癌治疗效果监测指标优于血清     

局域网组播的IGMP扩展认证模型设计

互联网组播技术可以从根本上改善网络带宽的利用率,使发送者和路由器的处理消耗以及发送延迟降到最低的程度。IGMP是一种应用比较广泛的组播用户组管理协议,但是本身没有认证功能。PAP（密码认证协议）提供了一种简单的可以使端对端使用2次握手建立身份验证的协议。论文提出一种IGMP改进方案,把PAP认证协议添加在IGMP v3协议的扩展字段中,用于组播路由的最后一跳认证。     

Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy

Obstructive sleep apnea (OSA) is characterized by nocturnal breathing intermissions resulting in oxidative stress and eventually, a low-grade systemic inflammation. The study aimed to investigate the impact of positive airway pressure (PAP) therapy on the inflammatory milieu as measured by monocyte and T cell phenotypic alterations. Participants were assessed for their OSA severity before PAP therapy and about six months later, including patient-reported outcome and therapy usage by telemetry readout. The distributions of the CD14/CD16-characterized monocyte subsets as well as the CD4/CD8-characterized effector T cell subsets with regard to their PD-1 and PD-L1 expression were analyzed by flow cytometry from blood samples. Data of 25 patients revealed a significant reconstitution of the monocyte subset distribution and a decrease in PD-L1 expression on pan-monocytes and CD8⁺ T cells without an association to initial AHI and overweight. The PD-1 expression was still increased on T cell subsets, especially on CD4⁺ TH17/22 cells. We conclude that PAP therapy might have a rapid effect on the monocyte phenotype and overall PD-L1 expression levels. However, T cell immune alterations especially on TH17/22 cells persist longer, indicating an ongoing disturbance of the adaptive immune system.     

二茂铁-肽衍生物的合成及对木瓜蛋白酶的检测

以二茂铁为起始原料合成了二茂铁-肽衍生物Boc-HN-Fca-Gly-Gly-Tyr-Arg-OMe,采用循环伏安法对Boc-HN-Fca-Gly-Gly-Tyr-Arg-OMe在乙醇溶液中的电化学性质进行了检测,并利用四肽H2N-Gly-Gly-Tyr-Arg-OMe可通过氢键与木瓜蛋白酶结合的性质,通过快速、灵敏、简单的生物电化学方法完成了其对木瓜蛋白酶的检测识别。     

前列腺酸性磷酸酶在胃癌中的表达及其体外诱导抗胃癌免疫应答

目的检测前列腺酸性磷酸酶(PAP)在胃癌中的表达水平,探讨PAP作为胃癌主动免疫治疗新靶点的可能性。方法分别用RT-PCR和Western blot方法检测胃癌细胞系中PAP mRNA和PAP蛋白的表达;用免疫组织化学方法检测胃癌组织中PAP的表达。利用PAP表位肽对胃癌患者的PBMCs进行体外诱导,ELISA法检测PAP特异性IFN-γ分泌水平;51Cr释放法检测CTLs的细胞毒活性。结果3种胃癌细胞(MKN-7、MKN-28和MKN-45)表达PAP mRNA,其中MKN-28和MKN-45表达PAP蛋白,胃癌组织中PAP呈阳性表达。从2/4胃癌患者PBMCs中诱导出的PAP多肽特异性CTLs可特异性杀伤HLA-A24+/PAP+的胃癌细胞MKN-45,CTLs的细胞毒活性依赖于CD8+T淋巴细胞。结论PAP有可能成为胃癌特异性免疫治疗的新靶点。