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1.
Probabilistic analysis of a static frame model 总被引:1,自引:0,他引:1
This paper describes our efforts during our participation in the Sandia Validation Workshop. The focus of the paper is the calibration of material models and simulation of random fields to characterize the variations of material properties across spatial field. Both parametric and non-parametric methods were used to represent uncertainty. Part of the challenge of this problem is the small amount of data that is available for the necessary probabilistic analyses in support of calibration, validation, accreditation and prediction activities. The analysis methods and corresponding results are described. 相似文献
2.
The effects of key parameters on the preparation and regeneration of protoplast from the β-carotene-producing fungus Blakeslea trispora were discussed in this paper, including the combination of various enzymes, mycelial age, digesting time and temperature,
pH value, osmotic stabilizers, pretreatment, culture medium and culture method. Under the condition of mixed enzymes in osmotic
stabilizer (0.6 M NaCl) combined with 2% lysozyme, 3% cellulase and 3% snailase, the highest protoplast yield, as high as
7.48×106 protoplasts/mL, was obtained when mycelial age was 60 h at pH 5.0–6.0 with digesting for 14–16 h at 28 °C. After
purification of the obtained protoplasts, they were regenerated in PDA regenerative medium using bilayer plate culture method.
To validate the usability of the protoplasts, a novel plasmid with green fluorescent protein (GFP) was used in transformation
for easy visual observation. The results showed that the protoplasts prepared by the optimized method were active and applicable
in further gene manipulation experiments.
This work was presented at 13
th
YABEC symposium held at Seoul, Korea, October 20–22, 2007. 相似文献
3.
Mike Schofield 《Quality and Reliability Engineering International》1992,8(6):549-555
Because of the growing demand for increasingly complex computer-based systems there is now an urgent need to provide tools to assist during the design of such systems. Formal specifications and formal methods provide such assistance but their widespread adoption has been hindered by the so-called ‘math fear’ and the perception that the tools are too difficult, too time consuming and too costly to use in a commercial environment. The aim of this article is to dispel the mystery surrounding the topic and to explain what formal methods are, how and why they are used, the benefits that accrue and why the technology should be accepted on a broader front. The application of formal methods to the design of computer-based systems will be discussed without resorting to jargon or mathematics. The discussion will concentrate more on the software content of systems but the arguments apply equally well to hardware. Some of the available tools will also be introduced. 相似文献
4.
Liaqat A. Khan Edward A. Wicklein E. C. Teixeira 《Canadian Metallurgical Quarterly》2006,132(7):741-746
A three-dimensional (3D) computational fluid dynamics (CFD) model of a contact tank is presented in this paper. The model results are compared against 3D velocities and flow through curve (FTC) data, representing a tracer concentration profile, from a 1:8 scale physical model. The objective is to demonstrate that CFD models can simulate both the FTC and the 3D velocity field quite well. Simultaneous validation of velocities and FTC is important in ascertaining the predictive capabilities of CFD models, as physical model studies indicate that different baffle arrangements can lead to similar FTCs. Therefore, a good prediction of only FTC, as presented in previous 3D CFD model studies, does not necessarily imply a correct simulation of the flow field. 相似文献
5.
Preliminary results of ENVISAT RA-2-derived water levels validation over the Amazon basin 总被引:1,自引:0,他引:1
Frédéric Frappart Stéphane Calmant Frédérique Seyler 《Remote sensing of environment》2006,100(2):252-264
Since the launch of the ENVISAT satellite in 2002, the Radar Altimetry Mission provides systematic observations of the Earth topography. Among the different goals of the ENVISAT Mission, one directly concerns land hydrology: the monitoring of the water levels of lakes, wetlands, and rivers. The ENVISAT Geophysical Data Records products contain, over different type of surfaces, altimeter ranges derived from four specialized algorithms or retrackers. However, none of the retrackers are intended to the processing of the radar echoes over continental waters. A validation study is necessary to assess the performances of the different ENVISAT-derived water levels to monitor inland waters. We have selected four test-zones over the Amazon basin to achieve this validation study. We compare first the performances of these retracking algorithms to deliver reliable water levels for land hydrology. Comparisons with in-situ gauge stations showed that Ice-1 algorithm, based on the Offset Centre of Gravity technique, provides the more accurate water stages. Second, we examine the potentiality to combine water levels derived from different sensors (Topex/Poseidon, ERS-1 and -2, GFO). 相似文献
6.
针对电子文档的安全性问题,分析了文档的各种数字签名情况,设计了一种能够完成多种签名方法,且能全面验证签名的文档签名系统。系统采用C#编程,从而提高了电子文档的安全性。 相似文献
7.
Antonia Vlahou 《Proteomics. Clinical applications》2013,7(5-6):384-387
Clinical Proteomics has traveled a long way pinpointing potential biomarkers for a variety of diseases. However, the absence of clinical implementation of proteomics findings has led to a frank evaluation and reconsideration of applied practices in biomarker discovery, recruitment of technological tools for biomarker verification and generation of new guidelines for data reporting. Nevertheless, considering the need for vast clinical resources for biomarker validation, the frequent lack of clear definitions of contexts of use, in combination to the biomarker “high offer,” progress toward biomarker implementation will even more require the adoption of an extensive open-minded approach: disease-focused networks are needed to ensure rapid exchange of information, initiation of appropriate studies, parallel validation of multiple biomarkers and sharing of valuable clinical resources. This viewpoint article targets to reflect on these issues and advocates the added value of multidisciplinary networks in biomarker development using bladder cancer as a paradigm. 相似文献
8.
孙志元 《电脑编程技巧与维护》2010,(8):38-40
对Excel数据处理进行了阐述,介绍了如何利用VBA编程实现Excel工作表中的数据检验和比较,并给出了相应的代码。 相似文献
9.
Some challenges in global land cover mapping: An assessment of agreement and accuracy in existing 1 km datasets 总被引:1,自引:0,他引:1
M. Herold P. Mayaux C.E. Woodcock A. Baccini C. Schmullius 《Remote sensing of environment》2008,112(5):2538-2556
Many investigators need and use global land cover maps for a wide variety of purposes. Ironically, after many years of very limited availability, there are now multiple global land cover maps and it is not readily apparent (1) which is most useful for particular applications or (2) how to combine the different maps to provide an improved dataset. The existing global land cover maps at 1 km spatial resolution have arisen from different initiatives and are based on different remote sensing data and employed different methodologies. Perhaps more significantly, they have different legends. As a result, comparison of the different land cover maps is difficult and information about their relative utility is limited. In an attempt to compare the datasets and assess their strengths and weaknesses we harmonized the thematic legends of four available coarse-resolution global land cover maps (IGBP DISCover, UMD, MODIS 1-km, and GLC2000) using the LCCS-based land cover legend translation protocols. Analysis of the agreement among the global land cover maps and existing validation information highlights general patterns of agreement, inconsistencies and uncertainties. The thematic classes of Evergreen broadleaf trees, Snow and Ice, and Barren show high producer and user accuracy and good agreement among the datasets, while classes of mixed tree types show high commission errors. Overall, the results show a limited ability of the four global products to discriminate mixed classes characterized by a mosaic of trees, shrubs, and herbaceous vegetation. There is a strong relationship between class accuracy, spatial agreement among the datasets, and the heterogeneity of landscapes. Suggestions for future mapping projects include careful definition of mixed unit classes, and improvement in mapping heterogeneous landscapes. 相似文献
10.
New refinements and validation of the MODIS Land-Surface Temperature/Emissivity products 总被引:10,自引:0,他引:10
Zhengming Wan 《Remote sensing of environment》2008,112(1):59-74
This paper discusses the lessons learned from analysis of the Moderate Resolution Imaging Spectroradiometer (MODIS) Land-Surface Temperature/Emissivity (LST) products in the current (V4) and previous versions, and presents eight new refinements for V5 product generation executive code (PGE16) and the test results with real Terra and Aqua MODIS data. The major refinements include considering surface elevation when using the MODIS cloudmask product, removal of temporal averaging in the 1 km daily level-3 LST product, removal of cloud-contaminated LSTs in level-3 LST products, and the refinements for the day/night LST algorithm. These refinements significantly improved the spatial coverage of LSTs, especially in highland regions, and the accuracy and stability of the MODIS LST products. Comparisons between V5 LSTs and in-situ values in 47 clear-sky cases (in the LST range from − 10 °C to 58 °C and atmospheric column water vapor range from 0.4 to 3.5 cm) indicate that the accuracy of the MODIS LST product is better than 1 K in most cases (39 out of 47) and the root of mean squares of differences is less than 0.7 K for all 47 cases or 0.5 K for all but the 8 cases apparently with heavy aerosol loadings. Emissivities retrieved by the day/night algorithm are well compared to the surface emissivity spectra measured by a sun-shadow method in two field campaigns. The time series of V5 MODIS LST product over two sites (Lake Tahoe in California and Namco lake in Tibet) in 2003 are evaluated, showing that the quantity and quality of MODIS LST products depend on clear-sky conditions because of the inherent limitation of the thermal infrared remote sensing. 相似文献