Chemical-Shift Perturbations Reflect Bile Acid Binding to Norovirus Coat Protein: Recognition Comes in Different Flavors

Bile acids have been reported as important cofactors promoting human and murine norovirus (NoV) infections in cell culture. The underlying mechanisms are not resolved. Through the use of chemical shift perturbation (CSP) NMR experiments, we identified a low-affinity bile acid binding site of a human GII.4 NoV strain. Long-timescale MD simulations reveal the formation of a ligand-accessible binding pocket of flexible shape, allowing the formation of stable viral coat protein–bile acid complexes in agreement with experimental CSP data. CSP NMR experiments also show that this mode of bile acid binding has a minor influence on the binding of histo-blood group antigens and vice versa. STD NMR experiments probing the binding of bile acids to virus-like particles of seven different strains suggest that low-affinity bile acid binding is a common feature of human NoV and should therefore be important for understanding the role of bile acids as cofactors in NoV infection.     

New 1,3,4-Oxadiazole Derivatives of Pyridothiazine-1,1-Dioxide with Anti-Inflammatory Activity

Numerous studies have confirmed the coexistence of oxidative stress and inflammatory processes. Long-term inflammation and oxidative stress may significantly affect the initiation of the neoplastic transformation process. Here, we describe the synthesis of a new series of Mannich base-type hybrid compounds containing an arylpiperazine residue, 1,3,4-oxadiazole ring, and pyridothiazine-1,1-dioxide core. The synthesis was carried out with the hope that the hybridization of different pharmacophoric molecules would result in a synergistic effect on their anti-inflammatory activity, especially the ability to inhibit cyclooxygenase. The obtained compounds were investigated in terms of their potencies to inhibit cyclooxygenase COX-1 and COX-2 enzymes with the use of the colorimetric inhibitor screening assay. Their antioxidant and cytotoxic effect on normal human dermal fibroblasts (NHDF) was also studied. Strong COX-2 inhibitory activity was observed after the use of TG6 and, especially, TG4. The TG11 compound, as well as reference meloxicam, turned out to be a preferential COX-2 inhibitor. TG12 was, in turn, a non-selective COX inhibitor. A molecular docking study was performed to understand the binding interaction of compounds at the active site of cyclooxygenases.     

小型机载激光通信系统设计与验证

针对空空宽带高速通信的需求，设计了小型化机载激光通信系统。仿真分析了300 km、2.5 Gb/s无线激光链路性能，并通过运动仿真台模拟机动环境测试了系统的跟踪与通信性能，其中粗跟踪误差为533.2 μrad(1σ)，精跟踪误差为3.6 μrad(1σ)，测试数据传输240 s，通信误码率为2.82×10-9。仿真与实验验证了该系统用于远距离空空无线激光通信的可行性。     

Genes Identification,Molecular Docking and Dynamics Simulation Analysis of Laccases from Amylostereum areolatum Provides Molecular Basis of Laccase Bound to Lignin

An obligate mutualistic relationship exists between the fungus Amylostereum areolatum and woodwasp Sirex noctilio. The fungus digests lignin in the host pine, providing essential nutrients for the growing woodwasp larvae. However, the functional properties of this symbiosis are poorly described. In this study, we identified, cloned, and characterized 14 laccase genes from A. areolatum. These genes encoded proteins of 508 to 529 amino acids and contained three typical copper-oxidase domains, necessary to confer laccase activity. Besides, we performed molecular docking and dynamics simulation of the laccase proteins in complex with lignin compounds (monomers, dimers, trimers, and tetramers). AaLac2, AaLac3, AaLac6, AaLac8, and AaLac10 were found that had low binding energies with all lignin model compounds tested and three of them could maintain stability when binding to these compounds. Among these complexes, amino acid residues ALA, GLN, LEU, PHE, PRO, and SER were commonly present. Our study reveals the molecular basis of A. areolatum laccases interacting with lignin, which is essential for understanding how the fungus provides nutrients to S. noctilio. These findings might also provide guidance for the control of S. noctilio by informing the design of enzyme mutants that could reduce the efficiency of lignin degradation.     

伪码连续波交会对接雷达信号处理机设计

介绍了某伪码连续波交会对接雷达信号处理机设计.在测距中,提出用噪声较小的载波相位△伪距测量值对伪距进行处理,得到高精度测距值.在测角中,提出了一种干涉仪天线阵结构和干涉仪解模糊算法,针对通道载波相位误差可能引起的解模糊错误,又提出了一种纠正方法,仿真显示,在发生解模糊错误的概率高达0.42的情况下,该方法仍然可以识别并纠正所有解模糊错误.实验表明,该信号处理机可以满足空间航天器交会对接的要求.     

某雷达数据处理有关问题分析

对某雷达数据处理应用中存在的一些特殊问题进行讨论分析,并就具体问题提出了解决的方法.     

主动雷达终端实时仿真软件的设计与实现

在主动雷达数据回放显示的基础上,通过使用D irectDraw和Intel IPP技术,设计了主动雷达终端实时仿真软件,包括信号处理、数据处理及终端显示等模块,极大地提高了软件的执行效率,使得整个仿真过程具有良好的实时性。本文介绍了该软件的设计及实现。     

INMARSAT 21世纪工程——全球个人移动卫星通信

本文概述INMARSAT 21世纪工程的规划及为实现这一规划,INMARSAT组织在本世纪末正在进行和即将进行的一系列卫星通信技术工作。着重阐述INMARSAT 21世纪工程的业务性能以及对三种卫星布局方案进行抉择的新动向。     

固定电话网的新趋势——固网信息服务系统

本文首先讨论了中国电信开展固网短信业务的必要性，然后分析了开展固网短信业务存在的问题，最后介绍了固网短信系统的构成。