Screen‐printed biosensor for allergens

Allergen levels in indoor environments, leading to many diseases, eg asthma, rhinitis and conjunctivitis, affect a large and increasing fraction of the population. A quite effective and inexpensive method of a rough but very rapid overall assessment of total allergen level in the environment has been developed. The method involved estimation of protein in allergen extracts by screen‐printed electrodes using two different techniques. The biosensor comprised a rhodinised carbon working electrode, a silver/silver chloride reference electrode and a carbon counter electrode. In the first method the enzyme protease reacted with allergen protein to release amino acid, which produced hydrogen peroxide in the presence of amino acid oxidase. This was detected amperometrically. The second method used potassium bromide as electrolyte and the electrode was subjected to dual potential. Bromine, released due to electrolysis at higher potential, was consumed by the allergen protein at lower potential. In the first method, a unique technique was used to microencapsulate the enzyme protease and immobilise it on the surface of the electrode by in‐situ polymerisation to avoid contact with the amino acid oxidase. A total of seven allergens were tested and the results gave a good correlation with the standard protein measurement method. Environmental specimens from indoors, schools and workplaces can be evaluated for the aeroallergens produced by dust mites, animal hairs, cockroach debris, pollens, etc as a means of determining the exposure risk. Copyright © 2005 Society of Chemical Industry     

Identification of gaps in mangrove forests with airborne LIDAR

Mangrove forests change frequently due to disturbances from tropical storms, frost, lightning, and insects. It has been suggested that the death and regeneration of trees in small gaps due to lightning may play a critical role in mangrove forest turnover; however, the large-scale quantification of spatial pattern and areas of gaps is lacking for investigating this issue. Airborne light detection and ranging (LIDAR) technology provides an effective way for identifying gaps by remotely obtaining direct measurements of ground and canopy elevations. A method based on an alternative sequential filter and black top-hat mathematical morphological transformation was developed to extract gap features. Comparison of identified gap polygons with raw LIDAR measurements and field surveys shows that the proposed method successfully extracted gap features in mangrove forests in Everglades National Park. There are 400–500 lightning gaps per square kilometer in mangrove forests at the study sites. The distribution of gap sizes follows an exponential form and the area of gaps with sizes larger than 100 m² account for 55–61% of the total area of gaps. The area of gaps in the mangrove forest in Everglades National Park is about 4–5% of the total forest area and the average gap formation rate is about 0.3% of the total forest area per year, indicating that lightning gaps play an important role in mangrove forest dynamics.     

The topographic normalization of hyperspectral data: implications for the selection of spectral end members and lithologic mapping

Compact Airborne Spectrographic Imager (CASI) hyperspectral data is used to investigate the effects of topography on the selection of spectral end members, and to assess whether the topographic correction improves the discrimination of rock units for lithologic mapping. A publicly available Digital Elevation Model (DEM), at a scale of 1:50,000, is used to model the radiance variation of the scene as a function of topography, assuming a Lambertian surface. Skylight is estimated and removed from the airborne data using a dark object correction. The CASI data is corrected on a pixel-by-pixel basis to normalize the scene to a uniform solar illumination and viewing geometry. The results show that topography has the effect of expanding end member clusters at times resulting in the overlap of clusters and that the correction process can effectively reduce the variation in detected radiance due to changes in local illumination. When topographic effects are embedded in the hyperspectral data, methods typically used for the selection of end members, such as the convex hull method, can miss end members or result in the selection of nonrepresentative pixels as end members. Thus, end members selected by some conventional methods are very likely “incomplete” or “nonrepresentative” if the topographic effect is embedded in the data. As shown in this study, the topographic correction can reveal hidden end members and achieve a better representation of end members via the statistical center of isolated clusters.     

秦山核电厂气载放射性释放的环境影响

本文评价了秦山核电厂气载放射性流出物对环境的影响。应用现场及风洞大气扩释实验结果和厂址周围的人口与食谱调查资料,估算了秦山核电厂在正常运行和事故条件下释放的气载放射性流出物对公众产生的个人有效剂量当量和集体有效剂量当量。计算结果表明,正常运行时厂址边界(0.5km)处的最大个人有效剂量当量为2.7×10~(-2)mSv/a,该剂量的大部分来自~(137)Cs 的食入(主要由地表湿沉积引起);80km 范围内的集体有效剂量当量为1.1人·Sv/a,归一化集体有效剂量当量为3.7人·Sv/GW(e)·a。文中还给出了事故情况下剂量估算结果。     

Aeromagnetic compensation using neural networks

Airborne magnetic surveys in geophysical exploration can be subject to interference effects from the aircraft. Principal sources are the permanent magnetism of various parts of the aircraft, induction effects created by the earth's magnetic field and eddy-current fields produced by the aircraft's manoeuvres. Neural networks can model these effects as functions of roll, pitch, heading and their time derivatives, together with vertical acceleration, charging currents to the generator, etc., without assuming an explicit physical model. Separation of interference effects from background regional and diurnal fields can also be achieved in a satisfactory way.     

机载雷达空时二维信号处理自适应权值训练的距离分段递推算法

本文针对机载预警雷达的杂波环境，分析了杂波数据的非平稳性，由此提出在距离上分段实现空时二维自适应处理（STAP）的方法，并通过分析杂波于空间的距离来确定分段的方法。在同时考虑实时性和处理器性能的前提下，给出了一种自适应权值计算的递推算法。理论分析和计算机仿真结果均表明，本文提出的分段递推算法是有效的。     

上海市吴淞工业区大气PM2.5水溶成分的元素分析及细胞毒性研究

通过ICP-MS测定了上海市吴淞地区大气PM2.5水溶成分金属元素含量,通过四唑盐(MTT)测定细胞存活率,通过超氧化歧化酶(SOD)和丙二醛(MDA)两个指标测定细胞氧化损伤,琼脂糖凝胶电泳测定细胞内DNA损伤,流式细胞仪(Flow cytometry)检测细胞凋亡和周期等实验研究了大气PM2.5水溶成分的细胞毒性.实验结果表明:PM2.5可溶成分中Zn元素含量最高,PM2.5在一定浓度范围内能导致细胞死亡,引起细胞的氧化损伤,影响DNA的复制,阻碍细胞增殖,但没有诱导细胞凋亡.PM2.5中可溶金属离子对细胞毒性表现为联合作用.     

基于WindowsCE的空降部队快速集结系统的设计

介绍基于Windows CE平台下的空降部队快速集结系统的开发。包括整个系统的组成以及软硬件平台的结构,通过PB软件对嵌入式Windows CE操作系统内核的定制．并应用visual Studio2008开发GPS定位、无线通信应用系统软件．实现空降部队快速集结系统各项功能。经过测试,基于Windows CE平台下的空降部队快速集结系统能够准确获得GPS信息。定位误差和响应时间均能满足设计要求。     

An economic and sensitive method for extracting chromium speciation in airborne inhalable dust,using a green sample treatment coupled with electrothermal atomic absorption

ABSTRACT

A new approach of cloud point extraction CPE procedure is optimized for hexavalent chromium determination in airborne dusts. Triton X-114 is used as a surfactant and 1-(2-pyridylazo)-2-naphthol as a specific complexing agent for the trivalent chromium’s removal from the aqueous phase to isolate hexavalent chromium compounds. The parameters influencing the extraction protocol (pH, surfactant concentration, and temperature are optimized. The obtained detection and quantification limits are 0.1 and 0.4 μg/L, respectively. The linearity is verified, with a regression coefficient close to 0.999 and the extraction recovery exceeds 99%. The method was successfully applied to analyze airborne samples collected from workplaces.     

基于小型无人飞行器的CAN总线余度技术研究

随着航空技术和微电子技术的发展,航空电子系统朝着综合化、智能化、模块化、标准化和系统化的方向发展,CAN总线由于其低成本、协议开放、实现简单、速率快、挂接节点多的特点得到了广泛应用,而这又对其可靠性提出了新的要求;由于飞行器上的众多设备均作为节点挂在CAN总线上,如果总线出现故障会对飞行器的安全带来灾难性的后果;作者在对CAN总线模型进行分析的基础上提出一种双余度CAN总线模型,并给出了实现方法,经过实践验证,该方法切实可行,并显著提高了系统可靠性.