A Role for Human Renal Tubular Epithelial Cells in Direct Allo-Recognition by CD4+ T-Cells and the Effect of Ischemia-Reperfusion

Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection.     

Electrospun microporous gelatin–polycaprolactone blend tubular scaffold as a potential vascular biomaterial

The work reported involved the fabrication of an electrospun tubular conduit of a gelatin and polycaprolactone (PCL) blend as an adventitia‐equivalent construct. Gelatin was included as the matrix for increased biocompatibility with the addition of PCL for durability. This is contrary to most of the literature available for biomaterials based on blends of gelatin and PCL where PCL is the major matrix. The work includes the assiduous selection of key electrospinning parameters to obtain smooth bead‐free fibres with a narrow distribution of pore size and fibre diameter. Few reports elucidate the optimization of all electrospinning parameters to fabricate tubular conduits with a focus on obtaining homogeneous pores and fibres. This stepwise investigation would be unique for the fabrication of gelatin–PCL electrospun tubular constructs. The fabricated microfibrous gelatin–PCL constructs had pores of size ca 50–100 μm reportedly conducive for cell infiltration. The measured value of surface roughness of 57.99 ± 17.4 nm is reported to be favourable for protein adhesion and cell adhesion. The elastic modulus was observed to be similar to that of the tunica adventitia of the native artery. Preliminary in vitro and in vivo biocompatibility tests suggest safe applicability as a biomaterial. Minimal cytotoxicity was observed using MTT assay. Subcutaneous implantation of the scaffold demonstrated acute inflammation which decreased by day 15. The findings of this study could enable the fabrication of smooth bead‐free microfibrous gelatin–PCL tubular construct as viable biomaterial which can be included in a bilayer or a trilayer scaffold for vascular tissue engineering. © 2019 Society of Chemical Industry     

复杂结构高耸建筑物爆破拆除切口研究

针对壁体材料多样、结构复杂的高耸建筑物的爆破拆除,通过实验研究确定了爆破切口形式,运用力学理论推导了余留支撑体所对应的圆心角和爆破切口高度计算公式,并在工程实践中得到验证.对爆破拆除类似建筑物具有较大的实用价值.     

简析Ⅲ类PHC管桩的成因及加固补强处理方法

本文主要通过Ⅲ类PHC管桩的形成分析,经过各方论证采用了合适的处理方法,取得了较好的效果。     

筒体结构连续化模型的弹性动力时程分析

本文用连续化模型对高层筒体结构在地震作用下的动力时程反应进行分析．     

FE Simulation for Internal Inversion of Aluminium Alloy Tube

1 Introduction A double-walled invertube is difficult to fabricate other than applying inversion process. Such kind of invertube can be used as impact energy absorbing element in transport vehicles, like aircrafts and automobiles. Reddy T Y[1] did the analysis of Internal inversion and calculated the inversion load. Reid[2] et al. examined nosing and internal inversion of cold drawn steel tube by the finite element package ABAQUS. Recently, Rosa P A[3] et al. investigated Internal invers…     

美国西雅图先锋广场历史保护区的形成

考察美国西雅图先锋广场历史保护区形成的过程,可以看出城市历史文化遗存的保护是经济与历史文化合力作用的结果,公众参与在城市历史文化保护工作中发挥着重要的作用。我国城市经济高速发展,保护与发展的矛盾冲突还没得到很好解决,先锋广场的例子对我们具有一定的参考价值。     

可膨胀波纹管技术在韦15-19井的应用

可膨胀管包括可膨胀实体管和可膨胀波纹管，主要用于在不减小井眼尺寸的情况下封堵各种复杂地层，对损坏套管进行补贴修复，作为各种井筒封隔器以及延长技术套管长度。实体管和波纹管两者膨胀机理不一样，相比之下，可膨胀波纹管技术具有膨胀工艺简单，作业周期短的优点，该技术一直由俄罗斯掌握。中国石化勘探开发研究院经过多年的潜心研究，终于形成了具有自主知识产权的可膨胀波纹管产品及其配套的工艺技术，新研制的可膨胀波纹管壁厚8mm，抗内压强度35MPa，抗外挤强度10MPa，并在江苏油田韦15—19井进行了漏层封堵现场试验，经过扩眼、管串下入、水力膨胀、机械膨胀等工艺后获得圆满成功，标志着我国已经打破了国外公司对该技术的垄断，填补了国内在该领域的技术空白。     

安居水电厂灯泡贯流式水轮发电机组的试运行

本文对安居水电厂2台灯泡贯流式水轮发电机组自投入试运行以来,出现的受油器壳体开裂、导叶接力器缸体和活塞拉伤、转轮桨叶传动拐臂连接销脱落、导叶外轴承座漏水、主轴工作密封漏水侵入水导轴承、锁锭装置固定螺钉剪断等故障的经过、原因分析及其处理办法进行了叙述。     

脱苯工艺的节能改造

焦化厂富油脱苯工艺中，管式炉法较蒸汽法节能。本溪钢铁公司焦化厂产焦１８０万ｔ／ａ的装置，其富油脱苯工艺由蒸汽法改为管式炉法后，每年可节煤７０００ｔ。