Expression of RCAS1 Correlates with Urothelial Bladder Cancer Malignancy

RCAS1 is a protein that participates in regulation of the tumor microenvironment and its immune responses, all in order to evade the immune system. The aim of this study was to analyze RCAS1 expression in urothelial bladder cancer cells (and in fibroblasts and macrophages of the tumor stroma) and its relationship with the histological pattern of malignancy. Eighty-three postcystectomy patients were enrolled. We analyzed the histological maturity (grade), progress (pT stage), tissue invasion type (TIT), nonclassic differentiation number (NDN), and the ability to metastasize (pN). The expression of RCAS1 protein was analyzed by immunohistochemistry. Indicators of histological malignancy were observed solely in association with the RCAS1 expression in cells in the border parts (BPs) of the tumor. Histological malignancy of the tumor, indicated by the pT and pN, and metastasis-free survival time, correlated significantly with RCAS1 expression in tumor neoplastic cells, whereas malignancy determined by grade, TIT, and NDN correlated with RCAS1 expression in fibroblasts and macrophages in the tumor microenvironment. These findings suggest that the increased RCAS1 expression depends on its cellular source and that RCAS1 expression itself is a component of various signaling pathways. The immune escape occurs within the tumor BPs, where the increase in the RCAS1 expression occurs within tumor cells and stromal cells in its microenvironment. We conclude that the histological pattern of tumor malignancy, indicated by grade, TIT, NDN, pT, and pN is a morphological indicator of immune escape.     

Role of Extracellular Vesicles in Epithelial Ovarian Cancer: A Systematic Review

Extracellular vesicles (EVs) are a heterogeneous group of cell-derived submicron vesicles released under physiological or pathological conditions. EVs mediate the cellular crosstalk, thus contributing to defining the tumor microenvironment, including in epithelial ovarian cancer (EOC). The available literature investigating the role of EVs in EOC has been reviewed following PRISMA guidelines, focusing on the role of EVs in early disease diagnosis, metastatic spread, and the development of chemoresistance in EOC. Data were identified from searches of Medline, Current Contents, PubMed, and from references in relevant articles from 2010 to 1 April 2020. The research yielded 194 results. Of these, a total of 36 papers, 9 reviews, and 27 original types of research were retained and analyzed. The literature findings demonstrate that a panel of EV-derived circulating miRNAs may be useful for early diagnosis of EOC. Furthermore, it appears clear that EVs are involved in mediating two crucial processes for metastatic and chemoresistance development: the epithelial–mesenchymal transition, and tumor escape from the immune system response. Further studies, more focused on in vivo evidence, are urgently needed to clarify the role of EV assessment in the clinical management of EOC patients.     

β放射性气体浓度计的新分度方法

本文介绍β放射性气体浓度计的一种新的分度方法——泄漏元件排气法。其特点是一只元件可分度放射性气体浓度计的许多读出数(甚至是几个量程),操作方便。     

熔敷金属扩散氢逸出特性研究

采用酒精法测量扩散氢 ,研究了焊条熔敷金属扩散氢含量以及在测氢过程中扩散氢逸出量与逸出时间的关系 (逸出特性 )。研究结果表明 ,熔敷金属扩散氢逸出特性受扩散氢含量、焊道数、熔敷金属化学成分和表面状态等多种因素的影响。熔敷金属扩散氢 (HDo)可以分为快速逸出扩散氢 (HDq)和慢速逸出扩散氢(HDs)两部分     

重组G145R HBsAg亲和纯化方法的建立及其应用

目的建立重组乙型肝炎病毒G145R变异HBsAg抗体亲和纯化方法。方法用抗-HBs单克隆抗体(D12- McAb)制备亲和层析胶,对2A8细胞(分泌G145R变异HBsAg)培养上清盐析物进行亲和纯化。采用SDS-PAGE、Western blot及ELISA,对纯化产物的纯度、特异性、含量和回收率进行鉴定,并与同法纯化的HBsAg阳性血清及r-wHBsAg提取物进行比较。结果D12-McAb对重组真核表达G145R变异HBsAg、HBsAg阳性血清及r-wHBsAS三者具有相似的亲和性,产物纯度分别为90.3%、95.2%和93.1%,回收率分别为43.3%、72.0%和66.4%。结论已成功地建立了重组G145R变异HBsAg抗体亲和纯化方法,为G145R变异以及其他HBV免疫逃逸变异感染的深入研究奠定了重要的技术基础。     

钛对熔敷金属扩散氢逸出特性及组织的影响

利用酒精法测氢技术、三点弯曲法试验，并结合显微分析和断口分析方法探讨了铣含量对熔敷金属扩散氢逸出特性、焊缝组织以及断口形貌的影响。研究结果表明，随着钛含量的增加，熔敷金属扩散氢含量以及扩散氢逸出速率随之下降，焊缝组织中针状铁素体量明显增多，断口纤维率随之增加。     

基于分布式结构的弹射生理损伤指标测试系统

为提升飞行员弹射救生系统试验中人体生理损伤指标的测试能力和测试可靠性,结合航空仿真试验假人的结构,提出了分布式测试系统的设计、配置方案,设计了系统触发及同步控制电路,试验结果表明,系统能够在不影响假人生物仿真性能的前提下,可靠实现弹射试验人体生理损伤指标的测试.     

机动目标的跟踪与反跟踪

以WGS－84标准的地心坐标系作为统一坐标系,通过建立机动目标的跟踪模型,实时预测机动目标的轨迹,并提出了机动目标的跟踪与反跟踪策略。首先考虑了单目标跟踪问题,提出了基于改进的MeanShift算法的目标跟踪模型,使用此模型提取聚类点,并对这些聚类点进行B样条曲线拟合,得到光滑的航迹。其次考虑了两目标跟踪问题,建立了基于最近邻及改进MeanShift算法的目标跟踪模型,利用最小二乘法对航迹数据进行二次曲线拟合,分析机动目标加速度变化规律,并通过判断拟合曲线上的点与球面位置关系,提出了两种着落点预测方法。最后分析了机动目标如何反雷达跟踪的问题,提出了反跟踪策略。     

液氮温区固-液复合绝缘气体小桥对沿面放电的影响

采用IEC脉冲电流法(IEC60270标准)对液氮温区超导复合绝缘沿面放电现象及其影响因素进行了研究。在六面屏蔽的局部放电实验室内,研究了不同放电条件对放电起始电压、放电量、击穿电压等的影响,进而研究了其作用机理,并用有限元方法开展了相关计算。实验数据表明,不同放电条件明显影响放电重复率、平均放电量、最大放电量等,放电产生的气泡越容易逸出,放电越严重;液氮的流动使放电起始电压有一定的升高,但对最终击穿电压影响不大;在放电通道上施加阻挡之后可明显提高最终的闪络击穿电压。     

EFFECT OF ESCAPE DEVICE FOR SUBMERGED FLOATING TUNNEL （SFT） ON HYDRODYNAMIC LOADS APPLIED TO SFT

This paper presents a potential approach to settle the problem of surviving major safety accidents in Submerged Floating Tunnel (SFT) that detachable emergency escape devices are set up outside SFT. The Computational Fluid Dynamics (CFD) technology is used to investigate the effect of emergency escape devices on the hydrodynamic load acting on SFT in uniform and oscillatory flows and water waves by numerical test. The governing equations, i.e., the Reynolds-Averaged Navier-Stokes (RANS) equations and standard turbulence equations, are solved by the Finite Volume Method (FVM). Analytic solutions for the Airy wave are applied to set boundary conditions to generate water wave. The VOF method is used to trace the free surface. In uniform flow, hydrodynamic loads, applied to SFT with emergency escape device, reduce obviously. But, in oscillatory flow, it has little influence on hydrodynamic loads acting on SFT. Horizontal and vertical wave loads of SFT magnify to some extend due to emergency escape devices so that the influence of emergency escape devices on hydrodynamic loads of SFT should be taken into consideration when designed.