Supramolecular Assembly of Aminoethylene‐Lipopeptide PMO Conjugates into RNA Splice‐Switching Nanomicelles

Phosphorodiamidate morpholino oligomers (PMOs) are oligonucleotide analogs that can be used for therapeutic modulation of pre‐mRNA splicing. Similar to other classes of nucleic acid‐based therapeutics, PMOs require delivery systems for efficient transport to the intracellular target sites. Here, artificial peptides based on the oligo(ethylenamino) acid succinyl‐tetraethylenpentamine (Stp), hydrophobic modifications, and an azide group are presented, which are used for strain‐promoted azide‐alkyne cycloaddition conjugation with splice‐switching PMOs. By systematically varying the lead structure and formulation, it is determined that the type of contained fatty acid and supramolecular assembly have a critical impact on the delivery efficacy. A compound containing linolenic acid with three cis double bonds exhibits the highest splice‐switching activity and significantly increases functional protein expression in pLuc/705 reporter cells in vitro and after local administration in vivo. Structural and mechanistic studies reveal that the lipopeptide PMO conjugates form nanoparticles, which accelerate cellular uptake and that the content of unsaturated fatty acids enhances endosomal escape. In an in vitro Duchenne muscular dystrophy exon skipping model using H2K‐mdx52 dystrophic skeletal myotubes, the highly potent PMO conjugates mediate significant splice‐switching at very low nanomolar concentrations. The presented aminoethylene‐lipopeptides are thus a promising platform for the generation of PMO‐therapeutics with a favorable activity/toxicity profile.     

X80焊管H_2S环境应力腐蚀开裂行为研究

采用三点弯曲加载法和微电极扫描测试技术,研究了X80高强度级管线钢及其焊缝区的抗H2S环境应力腐蚀开裂(SSCC)行为。测定了焊接接头的显微硬度分布,分析了显微组织结构、力学性能及抗腐蚀性能之间的相关性。研究表明,热影响区(HAZ)对应力腐蚀开裂最为敏感,母材的纵向和横向取样对应力腐蚀敏感性影响不显著,薄壁管材比厚壁管材有较好的抗H2S环境应力腐蚀性能。HAZ晶粒粗大、硬度高,局部腐蚀倾向大,由此导致该区SSCC敏感性高。     

具有细小组织的高强度和高延性钢管的开发

介绍了日本川崎制铁公司技术研究所开发出的电焊钢管采用热张力减径工艺的特性 ,及这种钢管的高强度、高延性等基本特性。用这种钢管制造的汽车零部件 ,经弯曲、冲击和疲劳特性等实验 ,证明具有良好的机械加工性能 ,是制造汽车用零部件的优良材料。     

螺旋焊管在线自动测径装置

螺旋焊管在线测径是生产高标准焊管的重要检测装置，可以实时监控钢管管径。选用CCD传感器与计算机连接，组成钢管测径装置，能够较好地达到对钢管管径实时监控的目的。     

Limit load analysis of strength undermatched welded T-joints under bending

An increasing amount of laser beam welded T‐joints (e.g. skin‐stringer) of aluminium alloys are now in use in advanced fuselage applications designed as ‘integral structures’ for weight and cost savings. It is known that weld joints generally show lower strength (undermatching) than base metal in both laser beam and friction stir welded joints of 6xxx series Al‐alloys. Damage tolerance considerations in terms of the residual strength of such joints require limit load solutions to be used in engineering fitness‐for‐service (FFS) analysis. The paper, therefore, provides an upper bound limit load solution in closed form for welded T‐joints (idealized) with strength undermatching and subject to a bending moment. In addition to the necessary requirements of the upper bound theorem, the kinematically admissible velocity field chosen leads to a stress field, which satisfies the equilibrium equations and some stress boundary conditions in the plastic zone. This is an advantage of the solution and, therefore, it is expected that the upper bound obtained is close to the exact limit load of such joints.     

汽车用焊接钢管的发展

介绍了汽车用焊接钢和产品品种，国内皮类管的生产现状及市场需求状况；国外汽车用焊管最新发展情况。建议国内汽车用焊管应该是定点供料，定点生产，设备配套，完善工艺，建立从炼钢，轧制到制管的质量管理体制。     

钢带试样位向及拉伸性能的探讨

从碳素结构钢热轧钢带各向异性的事实出发 ,阐述不同试样位向导致同一钢带不同的拉伸性能 ,因而影响钢带合格与否的判定。在全面考虑钢带和焊管标准的基础上 ,提出解决钢带判定的两种方法。同时 ,还对标准中存在的缺点作了说明 ,提出改进意见 ,供标准修订时参考。     

一般应力比时焊接结构S－N曲线的预测方法

本文考虑了焊接结构的几何形状、受载形式、应力比、残余应力、尺寸效应以及材料等因素对疲劳寿命的影响，利用Ｐｅｔｅｒｓｏｎ公式和极值疲劳切口系数概念，建立了一般应力比时焊接结构Ｓ－Ｎ曲线的预估模型．文中对几类典型的焊接结构的Ｓ－Ｎ曲线进行了预测，预测值与实验结果符合较好．本文方法可以大量减少疲劳实验，具有一定的工程实用价值．     

焊接铁网石箱(GABION)挡土墙设计与施工

焊接铁网石箱是采用镀锌的轻质铁线和先进的焊接工艺制造而成，可用于水利、公路、建筑工程。本文就焊接铁网石箱在水利工程挡土墙中的应用设计、制造工艺以及施工方法进行了详尽地介绍。     

Fatigue crack growth analysis of welded aluminium RHS T-joints with manipulated residual stress level

The fatigue life of a welded aluminium T‐joint made from beams with rectangular hollow section (RHS) has been predicted using a crack propagation analysis and compared with experimental results from joints with different residual stress levels. To include the effect of the residual stresses, the stress ratio was calculated at the weld toe and, via Walker's equation, introduced into the analysis. How to obtain the Walker exponent has been discussed in detail. The introduction of a stress ratio at the weld toe provides good agreement between the experimentally and analytically found S–N curves. The effect of the residual stress was successfully included in the analysis.