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1.
ULK1 (unc-51 like autophagy activating kinase 1), a mammalian serine/threonine kinase, is a key component of
autophagy initiation complex and helps to induce all types of autophagy. Canonical autophagy is a process in which,
through the interactions of a series of autophagy-related proteins, damaged organelles or misfolded proteins are
engulfed by autophagosomes and then merged with lysosomes to be degraded. Thus, canonical autophagy is an
important constituent part of the cellular “quality control.” Besides, accumulating evidence indicates that ULK1 exerts
autophagy-independent effects in a cell-specific manner. For example, ULK1 facilitates neurite elongation through the
regulation of endoplasmic reticulum (ER)–Golgi trafficking in neurons, stimulates phosphopentose pathway to help
NADPH (nicotinamide adenine dinucleotide phosphate hydrogen) production, and acts as a duplex regulator in type
I IFN (type I interferon) induced innate immune response. Considering the importance and diversity of ULK1 in
various biological processes, this review aims to present a comprehensive overview of autophagy and non-autophagy
related functions of ULK1 in a variety of human physiological, pathological, and disease processes. 相似文献
2.
Sebastian Sjoqvist Kentaro Otake Yoshihiko Hirozane 《International journal of molecular sciences》2020,21(24)
There is a lack of reliable biomarkers for disorders of the central nervous system (CNS), and diagnostics still heavily rely on symptoms that are both subjective and difficult to quantify. The cerebrospinal fluid (CSF) is a promising source of biomarkers due to its close connection to the CNS. Extracellular vesicles are actively secreted by cells, and proteomic analysis of CSF extracellular vesicles (EVs) and their molecular composition likely reflects changes in the CNS to a higher extent compared with total CSF, especially in the case of neuroinflammation, which could increase blood–brain barrier permeability and cause an influx of plasma proteins into the CSF. We used proximity extension assay for proteomic analysis due to its high sensitivity. We believe that this methodology could be useful for de novo biomarker discovery for several CNS diseases. We compared four commercially available kits for EV isolation: MagCapture and ExoIntact (based on magnetic beads), EVSecond L70 (size-exclusion chromatography), and exoEasy (membrane affinity). The isolated EVs were characterized by nanoparticle tracking analysis, ELISA (CD63, CD81 and albumin), and proximity extension assay (PEA) using two different panels, each consisting of 92 markers. The exoEasy samples did not pass the built-in quality controls and were excluded from downstream analysis. The number of detectable proteins in the ExoIntact samples was considerably higher (~150% for the cardiovascular III panel and ~320% for the cell regulation panel) compared with other groups. ExoIntact also showed the highest intersample correlation with an average Pearson’s correlation coefficient of 0.991 compared with 0.985 and 0.927 for MagCapture and EVSecond, respectively. The median coefficient of variation was 5%, 8%, and 22% for ExoIntact, MagCapture, and EVSecond, respectively. Comparing total CSF and ExoIntact samples revealed 70 differentially expressed proteins in the cardiovascular III panel and 17 in the cell regulation panel. To our knowledge, this is the first time that CSF EVs were analyzed by PEA. In conclusion, analysis of CSF EVs by PEA is feasible, and different isolation kits give distinct results, with ExoIntact showing the highest number of identified proteins with the lowest variability. 相似文献
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继第二、三期的专题连载(一)和(二)之后,这次介绍门票系统的扩展和系统的优势,并且给出了各种门票的对比表。 相似文献
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可拓市场的类型与实现方式研究 总被引:7,自引:0,他引:7
根据可拓市场的概念,探讨了在不同可拓变换下可拓市场的类型及实现可拓市场的方式。这些研究为企业开拓市场提供了有效的形式化方法。 相似文献
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Effect of Cerium on Expression and Activity of MMP-9 from Human Carcinoma of Bladder Cell Line 总被引:1,自引:0,他引:1
Rareearthshaveledtowidespreadinterestsinlifescience ,whilethescientistsdoresearchesdeeplyinmedicalandbiochemicalfields .Rareearthsplaypotentialrolesininhibitingcancer ,andalargenum berofscientistsdedicatethemselvestoit[1] .Thema trixmetalloproteinases (MMPs)areafamilyofatleast1 7humanzinc dependentendopeptidasesthatareca pableofdegradingalmostallextracellularmatrix(ECM)components .Theyareessentialinmanyphys iologicalprocessesandseveralpathologicalconditions ,suchastumorprogression .MMPsare… 相似文献