中原高温高盐油藏疏水缔合聚合物凝胶调剖剂研究

针对中原油田高温(>80℃)、高盐度(>50 g/L)、高硬度(>1 g/L)砂岩油藏,研制了地下成胶的高热稳定性聚合物凝胶调剖剂。所用聚合物为疏水缔合聚合物AP-P4,M=9.0×106,HD=27%,疏水基摩尔分数0.2%;交联剂为可生成酚醛树脂的3种化合物;调剖剂胶液用矿化度160 g/L的马寨油田污水配制;实验温度95℃。根据形成的凝胶黏度(95℃,1.1 s-1)确定交联剂各组分用量为:MZ-YL 0.429%,MZ-BE 0.060%,MZ-XS 0.012%;酸度调整剂用量0.12%;成胶时间10~15小时。2.5、3.0、3.5 g/L AP-P4的凝胶在95℃老化100天后黏度保持在~40 Pa.s及以上。初配制胶液在3000 r/min下剪切15分钟后黏度降低87.5%~89.0%,但成胶后和老化过程中凝胶黏度只比未剪切样降低17.1%~6.6%。3.5 g/L AP-P4的调剖剂对~1μm2的6支储层岩心的堵塞率在88.1%~95.8%范围,平均93.3%,造成的残余阻力系数在15.2~28.6范围,平均19.8,使渗透率级差2.3~8.0的4组双人造岩心的注水流量比发生不同程度的反转。认为该凝胶调剖剂高温下稳定性好的原因,是强化学交联密度低,因而凝胶脱水收缩作用弱。图2表5参5。     

Na+ and/or Cl− Toxicities Determine Salt Sensitivity in Soybean (Glycine max (L.) Merr.), Mungbean (Vigna radiata (L.) R. Wilczek), Cowpea (Vigna unguiculata (L.) Walp.), and Common Bean (Phaseolus vulgaris L.)

Grain legumes are important crops, but they are salt sensitive. This research dissected the responses of four (sub)tropical grain legumes to ionic components (Na⁺ and/or Cl⁻) of salt stress. Soybean, mungbean, cowpea, and common bean were subjected to NaCl, Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and a “high cation” negative control for 57 days. Growth, leaf gas exchange, and tissue ion concentrations were assessed at different growing stages. For soybean, NaCl and Na⁺ salts impaired seed dry mass (30% of control), more so than Cl⁻ salts (60% of control). All treatments impaired mungbean growth, with NaCl and Cl⁻ salt treatments affecting seed dry mass the most (2% of control). For cowpea, NaCl had the greatest adverse impact on seed dry mass (20% of control), while Na⁺ salts and Cl⁻ salts had similar intermediate effects (~45% of control). For common bean, NaCl had the greatest adverse effect on seed dry mass (4% of control), while Na⁺ salts and Cl⁻ salts impaired seed dry mass to a lesser extent (~45% of control). NaCl and Na⁺ salts (without Cl⁻) affected the photosynthesis (P_n) of soybean more than Cl⁻ salts (without Na⁺) (50% of control), while the reverse was true for mungbean. Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and NaCl had similar adverse effects on P_n of cowpea and common bean (~70% of control). In conclusion, salt sensitivity is predominantly determined by Na⁺ toxicity in soybean, Cl⁻ toxicity in mungbean, and both Na⁺ and Cl⁻ toxicity in cowpea and common bean.     

Contrasting Rootstock-Mediated Growth and Yield Responses in Salinized Pepper Plants (Capsicum annuum L.) Are Associated with Changes in the Hormonal Balance

Salinity provokes an imbalance of vegetative to generative growth, thus impairing crop productivity. Unlike breeding strategies, grafting is a direct and quick alternative to improve salinity tolerance in horticultural crops, through rebalancing plant development. Providing that hormones play a key role in plant growth and development and stress responses, we hypothesized that rootstock-mediated reallocation of vegetative growth and yield under salinity was associated with changes in the hormonal balance. To test this hypothesis, the hybrid pepper variety (Capsicum annuum L. “Gacela F1”) was either non-grafted or grafted onto three commercial rootstocks (Creonte, Atlante, and Terrano) and plants were grown in a greenhouse under control (0 mM NaCl) and moderate salinity (35 mM NaCl) conditions. Differential vegetative growth versus fruit yield responses were induced by rootstock and salinity. Atlante strongly increased shoot and root fresh weight with respect to the non-grafted Gacela plants associated with improved photosynthetic rate and K⁺ homeostasis under salinity. The invigorating effect of Atlante can be explained by an efficient balance between cytokinins (CKs) and abscisic acid (ABA). Creonte improved fruit yield and maintained the reproductive to vegetative ratio under salinity as a consequence of its capacity to induce biomass reallocation and to avoid Na⁺ accumulation in the shoot. The physiological responses associated with yield stability in Creonte were mediated by the inverse regulation of CKs and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid. Finally, Terrano limited the accumulation of gibberellins in the shoot thus reducing plant height. Despite scion compactness induced by Terrano, both vegetative and reproductive biomass were maintained under salinity through ABA-mediated control of water relations and K⁺ homeostasis. Our data demonstrate that the contrasting developmental and physiological responses induced by the rootstock genotype in salinized pepper plants were critically mediated by hormones. This will be particularly important for rootstock breeding programs to improve salinity tolerance by focusing on hormonal traits.     

Genome-Wide Analysis of the Late Embryogenesis Abundant (LEA) and Abscisic Acid-, Stress-, and Ripening-Induced (ASR) Gene Superfamily from Canavalia rosea and Their Roles in Salinity/Alkaline and Drought Tolerance

Canavalia rosea (bay bean), distributing in coastal areas or islands in tropical and subtropical regions, is an extremophile halophyte with good adaptability to seawater and drought. Late embryogenesis abundant (LEA) proteins typically accumulate in response to various abiotic stresses, including dehydration, salinity, high temperature, and cold, or during the late stage of seed development. Abscisic acid-, stress-, and ripening-induced (ASR) genes are stress and developmentally regulated plant-specific genes. In this study, we reported the first comprehensive survey of the LEA and ASR gene superfamily in C. rosea. A total of 84 CrLEAs and three CrASRs were identified in C. rosea and classified into nine groups. All CrLEAs and CrASRs harbored the conserved motif for their family proteins. Our results revealed that the CrLEA genes were widely distributed in different chromosomes, and all of the CrLEA/CrASR genes showed wide expression features in different tissues in C. rosea plants. Additionally, we introduced 10 genes from different groups into yeast to assess the functions of the CrLEAs/CrASRs. These results contribute to our understanding of LEA/ASR genes from halophytes and provide robust candidate genes for functional investigations in plant species adapted to extreme environments.     

再生高含盐水景观河道生态修复工程水质净化效果

通过对天津经济技术开发区泰达再生高盐水景观河道生态修复工程实际水体水质变化情况进行研究，分析了滨海盐碱地区高盐再生水景观河道水体生态修复工程的水质净化效果以及河道盐份动态变化规律。研究结果表明：水体中主要污染物COD浓度在54．15～94．15mg／L范围内变化，几乎没有去除；氖磷净化效果非常明显，它们的最大去除率分别为TN69．35％、NH3-N50．75％、NO3^--N71．89％、TP56．97％、po^3-4-p81．02％；河道水体全盐量（TDS）浓度在4300～5200mg／L范围内波动，TDS浓度有所升高，增加量不大，只有850mg／L左右，表明洗盐基本结束。由此说明生态修复工程对泰达再生高盐水景观河道水环境质量改善效果明显。     

Effect of potassic and phosphatic fertilizer type, fertilizer Cd concentration and zinc rate on cadmium uptake by potatoes

In some areas of southern Australia, cadmium (Cd) concentrations in excess of the Australian maximum permitted concentration (0.05 mg kg^–1 fresh weight) have been found in tubers of commercially grown potato (Solanum tuberosum L.) crops. Field experiments were therefore conducted in various regions of Australia to determine if Cd uptake by potatoes could be minimised by changes in either phosphorus (P), potassium (K) or zinc (Zn) fertilizer management.Changing the chemical form in which either P fertilizer (monoammonium phosphate, diammonium phosphate, single superphosphate and reactive rock phosphate) or K fertilizer (potassium chloride and potassium sulfate) were added to crops had little influence on tuber Cd concentrations. Fertilizer Cd concentrations also had little influence on tuber Cd concentrations, suggesting that residual Cd in the soil was a major contributor to Cd uptake by the crops on these soils.Addition of Zn at planting (up to 100 kg Zn ha^–1) significantly reduced tuber Cd concentrations at four of the five sites studied. However, the largest variation was between sites rather than between treatments, with site mean tuber Cd concentrations varying tenfold (from 0.018 to 0.177 mg Cd kg^–1 fresh weight). Factors associated with irrigation water quality at the sites, in particular the chloride concentration, appeared to dominate any effects of changing fertilizer type or Cd concentration.     

开封引黄灌区灌溉条件下稻田土壤盐分阈值研究

结合各生育期水稻耐盐度、各处理实际种前初始土壤含盐量和对应生育期最高的土壤含盐量,分析了各灌溉处理条件下的稻田土壤含盐量在水稻各生育期的动态变化规律和允许初始土壤含盐量阈值.结果表明:控制灌溉及控制灌溉加淋洗条件下,各水稻生育期土壤含盐量均有不同程度降低,随着淋洗水量的增加土壤含盐量逐渐降低,并获得了不同灌溉方式下的允许稻田初始土壤含盐量阈值.此结论对实际应用有指导意义.     

用工农业废料配制的两种堆肥培育观赏植物

由工农业废料配制的两种堆肥作为试验用的基质:C1——酿造啤酒等废料(酵母和麦芽)加修剪下的柠檬树枝;C2——橄榄研磨厂废水的固体级分加橄榄树叶。以每种堆肥与泥炭藓泥炭或商品基质(CS)按不同的比例混合,可配制成16种基质。尽管基质的物理和理化特性例如总孔隙度和电导率(EC)都是合适的,但由堆肥提供的养分(氮和钾)释放缓慢,对金盏花发育的影响尤为明显。另一方面,由于对盐分含量敏感,EC和氯化物浓度是影响蒲包草生长的主要因素。为供金盏花发育的适宜的基质可以通过含量高达75?与泥炭或高达50?与CS混合,以及高达50?与泥炭或CS混合使用。对蒲包花来说,基质中堆肥比例应较低,可用50?与泥炭或CS混合,也可以用25?与泥炭或CS混合使用。因此,只要基质至少含有25%的泥炭或CS,源于工农业废料的堆肥可作为培育观赏植物的泥炭和CS的替代品。     

应对长江口咸潮入侵的临界流量经验模型研究

1994年以来,长江口咸潮入侵严重威胁当地供水安全,随着长江上游水库群陆续建成,有关部门提出了通过上游水库群压咸调度保障长江口地区供水安全的方案。为明确压咸调度的目标流量(即临界流量),基于统计分析的方法,采用多元回归分析和主成分分析方法,选取实测站点数据建立了盐度-潮差-入海流量的相关关系统计模型,研究了咸潮发生的机理;通过模型分析多次咸潮入侵数据,确定临界流量为一个变化值,潮差290～335 cm下的临界流量在9 390～21 633 m³/s之间。研究表明,当长江口咸潮入侵时,单独依靠长江上游水库调度压咸所需的流量大,响应时间长,压咸效果不明显。因此,实际压咸调度应通过流域整体调度与本地蓄淡避咸水库共同作用应对咸潮入侵。     

非生育期春灌灌水量对土壤盐分变化的影响

通过田间小区试验,分析3个不同滴灌棉田春灌灌水量1350、1800和2250 m3/hm2对盐分变化的影响。试验表明:在相同灌水量下,宽行、窄行和膜间3个不同行距的脱盐效果,宽行最好;3个灌水量下,1800 m3/hm2的灌水量对盐分淋洗效果较好。此结果可为当地春灌提供指导,对制定灌溉制度有一定的借鉴作用。