Apoptotic Effects of Anthocyanins from Vitis coignetiae Pulliat Are Enhanced by Augmented Enhancer of the Rudimentary Homolog (ERH) in Human Gastric Carcinoma MKN28 Cells

Evidence suggests that augmented expression of a certain gene can influence the efficacy of targeted and conventional chemotherapies. Here, we tested whether the high expression of enhancer of the rudimentary homolog (ERH), which serves as a prognostic factor in some cancers, can influence the efficacy of anthocyanins isolated from fruits of Vitis coignetiae Pulliat, Meoru in Korea (AIMs) on human gastric cancer cells. The anticancer efficacy of AIMs was augmented in ERH-transfected MKN28 cells (E-MKN28 cells). Molecularly, ERH augmented AIM-induced caspase-dependent apoptosis by activating caspase-3 and -9. The ERH-augmented apoptotic effect was related to mitochondrial depolarization and inhibition of antiapoptotic proteins, XIAP, and Bcl-2. In addition, reactive oxygen species (ROS) generation was augmented in AIMs-treated E-MKN28 cells compared to AIMs-treated naïve MKN28 cells. In conclusion, ERH augmented AIM-induced caspase-dependent mitochondrial-related apoptosis in MKN28 cells. A decrease in expression of Bcl-2 and subsequent excessive ROS generation would be the mechanism for ERH-augmented mitochondrial-related apoptosis in AIMs-treated MKN28 cells. A decrease in expression of XIAP would be another mechanism for ERH-augmented caspase-dependent apoptosis in AIMs-treated MKN28 cells.     

Fluorescent Brightener 28 Removal by Ozonation or Advanced Oxidation Processes (O3/H2O2)

The aim of this work is to study the reaction of ozone and combined ozone/hydrogen peroxide mixtures with the fluorescent brightener 28 in dilute aqueous solution using controlled experimental conditions. The kinetics were also evaluated under various experimental conditions. The main ozonation by-products have been identified by High Pressure Anionic Exchange Chromatography (HPAEC) and Gas Chromatography coupled with Mass Spectrometry (GC-MS) techniques and a reaction pathway is proposed. In order to confirm this mechanism, melamine and s-triazine have been treated under the same reaction conditions and their decomposition pathways were studied.     

Assessment of a redox alkaline/iron-chelate absorption process for the removal of dilute hydrogen sulfide in air emissions

The oxidative absorption of hydrogen sulfide (H₂S) into a solution of ferric chelate of trans-1,2- diaminocyclohexanetetraacetate (CDTA) was studied in a counter-current laboratory column randomly packed with 15 mm plastic Ralu rings. The present investigation takes concern about the Kraft pulping situation where dilute H₂S concentrations are omnipresent in large-volume gas effluents. A fractional two-level factorial approach was instigated to determine the significance of six operating variables, namely the solution's alkalinity (pH; 8.5-10.5), the liquid mass flow rate (L;1.73-), the solution's ionic strength (I_C;0.01-), the gas mass flow rate (G;0.19-), the inlet H₂S concentration (C_H2S,0;70-430 ppm) and the initial ferric CDTA concentration (C_Fe,0;100 -). Initially, a Plackett-Burman design matrix of seven duplicated experiments revealed that pH is the leading factor controlling the H₂S conversion rate while the ionic strength and ferric CDTA concentration effects remained negligible within the factorial domain. Surface response analysis based on 11 duplicated factorial experiments plus 10 central composite trials revealed that the H₂S conversion significantly increases with liquid flow rate but decreases with growing H₂S load up. Further examination about the influence of ferric CDTA on H₂S absorption rate was set up over a broader concentration range (C_Fe,0;0- at pH of 9.5 and 10.5. It showed good potential at as H₂S conversion increased by a significant 25% for both pH values in comparison to pure alkaline solutions containing no ferric CDTA.     

基于STM32的校园能耗监控系统的以太网接口设计

本文针对校园能耗监控系统,介绍了一种基于STM32单片机和以太网控制器的以太网接口设计方案,阐述了STM32微处理器及ENC28J60芯片的功能和特点,并给出了系统的硬件和软件设计方案.根据该以太网接口设计一个在Web服务器上控制照明的测试,通过测试证明,该设计能够在校园能耗监控系统中进行信息的高速、远程传输,完成以太网接入功能.     

Effect of nitrogen on corrosion behavior of 28Cr-7Ni duplex and microduplex stainless steels in air-saturated 3.5 wt% NaCl solution

The corrosion behavior of 28Cr-7Ni-O-0.34N duplex stainless steels in air-saturated 3.5-wt% NaCl solution at pH 2, 7, 10 and 27 °C was studied by the potentiodynamic method. Two types of microstructures were investigated: the as-forged duplex and microduplex (average austenite grain size 5-16 μm) structures. The austenite volume fractions of the tested steels were between 0.35 and 0.64. The nitrogen effect on corrosion behaviors of both duplex and microduplex stainless steels were the same. At pH 2, the corrosion potential increased when the nitrogen content increased, however, corrosion current density as well as corrosion rate decreased. At pH 7 and 10, the effect of nitrogen on corrosion potential and corrosion rate could not be observed. Corrosion potential at pH 10 was lower than at pH 7. Pitting potential increased when the nitrogen content in the tested steels increased at all tested pH. For the nitrogen effect on the passive current density, it seemed that only at pH 2, the average passive current densities reduced when the nitrogen content increased. Nitrogen may have participated in the passive film or has been involved in the reaction to build up passive film. The ammonium formation and nitrogen enrichment at the interface metal/passive film with adsorption mechanism were discussed. The dissolute nitrogen might have combined with the hydrogen ions in solution to form ammonium ions, resulting in increasing solution pH. The steel could then easily repassivate, hence the corrosion potential and pitting potential would increase. However, the ammonium formation mechanism could not explain the decrease of corrosion potential in basic solution. Nitrogen enrichment at the metal/passive film interface with adsorption mechanism seemed to be an applicable consideration in increasing pitting potential. However, this mechanism did not involve the ammonium ion formation. In general, for the duplex and microduplex stainless steels tested, nitrogen increased the general corrosion resistances in acid solution and pitting corrosion resistance at all solution pH. Metallographic observation in both tested duplex and microduplex steels after pitting corrosion at all tested pH revealed that, the corroded structure in the tested steels without nitrogen alloying was austenite, but those with nitrogen alloying was ferrite. Even though ferrite had a higher chromium content than austenite but higher dissolved nitrogen in austenite than in ferrite may have increased the pitting resistance equivalent number (PRE) of austenite to be higher than that of ferrite.     

KYN28-12型铠装金属式封闭开关柜实训与注意事项

介绍KYN28-12型铠装金属式封闭开关柜的实训内容,分析学员没按技术规范和标准进行捡查、操作和检修的原因,并提出在实训过程中的注意事项.     

基于ZigBee的无线传感器网络平台

介绍了基于Zigbee的无线传感器网络,采用Atmegal28L和CC2420芯片设计实现了,一个基于Zigbee的无线传感器网络平台,详细阐述了系统架构设计,微控制器模块和射频模块硬件设计以及基于TinyOS的软件设计.实验测试该平台工作稳定可靠.     

Nand Flash存储管理在DSP系统中的实现

Nand Flash因其体积小、容量大、成本低、掉电数据不丢失等一系列优点已经在嵌入式系统中得以广泛应用,然而,由于Flash读写存储的编程特点以及其生产工艺与使用过程中坏块不可避免的问题,有必要对其进行存储过程管理,以使整个系统性能得以改善.本文介绍以K9F6408U0C为代表的Nand flash的存储管理及基于磨损均衡思想的存储管理算法在DSPTMS320F28x的系统中的程序实现.     

基于DS28E01的FPGA加密认证系统的设计

现在电子系统的设计成本越来越高,但是基于SRAM的FPGA的设计容易被克隆,本文针对这种现象,设计了一种基于MAXIM公司的DS28E01芯片的的加密认证系统,并成功应用于实际系统.     

基于CC2500的RFID网络控制器的设计

提出了一种基于CC2500射频收发芯片的低功耗2.45GHz有源RFID控制器解决方案.结合CC2500的空闲信道评估功能和时隙ALOHA算法,更加有效地解决了多标签的识别问题.针对现有RFID系统组网困难且成本较高的问题,采用ENC28J60和精简TCP/IP协议栈的方式,实现了RFID控制器的以太网通信功能.