粉葛愈伤组织不同极性提取物生物活性与功能成分分析

目的 为探究粉葛愈伤组织次生代谢产物的生物活性和功能成分。方法 采用系统溶剂法制备粉葛愈伤组织石油醚提取物(petroleum ether extract,PE)、乙酸乙酯提取物(ethyl acetate extract,EE)、甲醇提取物(methanol extract,ME)和水提取物(water extract,WE)等4种不同极性次生代谢产物,利用8株细菌、2个自由体系和1个细胞系测试四种提取物的抗菌、抗氧化和细胞毒活性,分析4种提取物中总黄酮、总酚、总多糖等主要功能成分含量与生物活性的相关性,并运用高效液相色谱法(high performance liquid chromatography,HPLC)检测其单体功能成分。结果 四种提取物的生物活性显著差异,其中EE对粪肠球菌、金黄色葡萄球菌、藤黄微球菌等革兰氏阳性菌作用突出,其最小抑菌浓度(minimum inhibitory concentration,MIC)为0.195~0.391 mg.mL_-1^,EE对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)自由基清除能力均最佳,其半抑制浓度(half maximal inhibitory concentration,IC₅₀)分别为0.2795和0.0640 mg.mL_-1^,EE对人肺癌细胞A549(human lung cancer cell)的抑制能力较佳,其半最大效应浓度(concentration for 50% of maximal effect,EC₅₀)为260.9 μg.mL_-1^{。总黄酮和总酚在}EE中含量均最高,分别为(273.89±7.48)μg.mg_-1^和(261.00±14.94)μg.mg_-1^,总多糖在WE中含量最高,达到(402.25±13.49)μg.mg_-1^,相关性分析显示,总黄酮为重要的抗菌和抗氧化活性成分。异黄酮单体功能成分在四种提取物中组成和含量差异均较大,且主要集中在EE和ME,其中EE以大豆苷(30.3428±1.4251)μg.mg_-1^{和大豆苷元}(45.7235±2.0230)μg.mg_-1^为主,ME以大豆苷(60.0125±3.9250)μg.mg_-1^{和染料木苷}(20.5655±1.3025)μg.mg_-1^为主。结论 粉葛愈伤组织次生代谢产物具有较强的抗菌、抗氧化活性以及微弱的细胞毒活性,主要含有大豆苷、染料木苷和大豆苷元等功能成分,该结果为粉葛愈伤组织次生代谢产物在食品与制药领域的开发利用提供了一定的参考。     

International Humanitarian Award.

The International Humanitarian Award recognizes extraordinary humanitarian services and activism by psychologists, including professional and volunteer work conducted primarily in the field with underserved populations. Award recipients are psychologists who, by their extraordinary service at a difficult time, improve the lives and contribute to the well-being of people in a large or small geographic area anywhere in the world. Award winners from 1998-2005 are listed. This document includes the citation awarded to Eduardo Almeida, the winner of the 2005 APA International Humanitarian Award. A brief biography and a selected bibliography of Almeida accompany the citation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Eduardo Almeida: International Humanitarian Award.

This document presents the citation awarded to Eduardo Almeida, recipient of the 2005 APA International Humanitarian Award. A brief biography and a selected bibliography of Almeida accompany the citation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Inhabiting the Body and the Spaces of Interaction

Wearable technologies create a tactile interface with the body of the user, providing a new model for architecture in which the surface between the subject and the object is almost seamless and sensory. As Valentina Croci explores, they also call on us to reconsider a more interdisciplinary mode of designing that accommodates a dynamic social dialogue between highly portable small-scale devices, their remote networks and the physical environment. Copyright © 2008 John Wiley & Sons, Ltd.     

Book reviews

This article describes the planning activity in Portugal in the middle of the twentieth century, notably the work of two exceptional planners, Etienne de Groër and Antão Almeida Garrett. The article moves from a first overview on the national context, to a focus on the two largest cities of Portugal, Lisbon, and Oporto. It was there that these two planners developed their most successful planning proposals. The planning activity of de Groër and Garrett is presented, first taken in isolation and then on a comparative basis. Issues addressed include the urban structure and the organization of the city, the street layout, and the system of zoning. It is argued that de Groër municipal plan for Lisbon and Garrett municipal plan for Oporto were able to leave a profound and lasting impact on these cities, being the most influential planning documents of the twentieth century in Lisbon and in Oporto.     

Salvador: The Struggle for Dialogue Within a Heritage City

Founded by the Portuguese in the mid-16th century as the colonial capital of Brazil, Salvador da Bahia on the northeast coast retains to this day a unique historical centre. Now a burgeoning metropolis, Salvador is also the country's third largest city with all the social, political and infrastructure problems and inequalities that accompany explosive urban growth. Sergio Ekerman , an architect and professor at the Federal University of Bahia (UFBA) in Salvador, describes how a lack of political will and consensus between private and public stakeholders is failing to produce the dialogue necessary for coherent urban development.