Inhibitory effects of Broccolini leaf flavonoids on human cancer cells

Broccolini (Brassica oleracea Italica × Alboglabra) is a hybrid between broccoli and Gai Lan, also known as Chinese broccoli and Chinese kale. The aim of this study was to assess the antitumor activity of Broccolini leaf flavonoids (BLF). Cell growth inhibition was evaluated using a standard colorimetric MTT assay, cellular morphology was observed using phase contrast microscopy and flow cytometry was introduced to further investigate cells apoptosis effect. The results showed that BLF possess a dose-dependent antiproliferative effects on four human cancer cell lines (SW480, HepG2, Hela, and A549) and apoptosis induction activity on SW480 cell line. Thus, the hybrid species Broccolini could be considered as a functional vegetable with potential in assisting for the treatment of four human cancers examined here.     

西兰苔凝集素的分离纯化及性质研究

本文采用硫酸铵沉淀法,分别以生理盐水,蒸馏水,pH=7.2的Tris-HCl缓冲液,pH=7.2的磷酸缓冲液为浸提液,对西兰苔凝集素进行提取,然后依次利用DEAE-52阴离子交换层析和SephadexG-75对凝集素粗品进行分离纯化,并对纯化后的西兰苔凝集素的部分性质进行研究。结果表明:pH=7.2的Tris-HCl缓冲液的提取率最高,为3.57±0.02%;纯化后的西兰苔凝集素分子量约为27 ku,糖含量为7.60±0.03%,其凝血活力为28;糖抑制试验表明,西兰苔凝集素的凝集活性受D-甘露糖和阿拉伯糖的抑制;热稳定性实验表明90℃以下凝集素的凝集活性不受温度影响,有较好的热稳定性。此外,西兰苔凝集素的最适pH范围是7~8,强酸强碱环境中其凝血活性明显下降。在含有Ca2+的溶液中其凝集活性有所增强,表明西兰苔凝集素活性受Ca2+影响较大。