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排序方式: 共有600条查询结果,搜索用时 46 毫秒
1.
牙鲆淋巴囊肿病的PCR诊断方法研究 总被引:14,自引:0,他引:14
以中国养殖牙鲆 (Paralichthysolivaceus)淋巴囊肿病毒 (Lymphocystisdiseasevirus,LCDVcn)主要衣壳蛋白 (majorcapsidprotein ,MCP)基因的中间保守序列为目标基因 ,设计了一对特异性引物。该引物可扩增出 172bp的病毒DNA片段 ,其最小DNA检出量为 0 0 183ng。用PCR法从人工感染淋巴囊肿病毒 3天的牙鲆血、鳃、肝、脾、肠、胃及自然发病牙鲆的肿瘤中 ,分别检测到了LCDV的存在。本实验结果证明 ,PCR法对于早期检测LCDV是十分有效的。 相似文献
2.
Ilkka Kuorinka Hannu Alaranta Ilkka Erich 《International Journal of Industrial Ergonomics》1995,15(6):437-446
During the years 1986–1989 The Finnish Work Environment Fund initiated a multicenter, intervention study (programme) to prevent musculoskeletal disorders at work. The programme consisted of four different projects comprising methodological, epidemiological, and interventive themes. The main results were as follows: (1) More than half of the occupational physiotherapists and physicians involved in the project felt that they did not have adequate training for testing the performance capacity of the musculoskeletal system. Several of the most commonly used musculoskeletal tests had either poor validity in predicting future musculoskeletal troubles or had poor reliability. (2) A controlled intervention study showed that active training of neck-shoulder muscles can prevent musculoskeletal troubles, even more than ergonomic actions. (3) It is possible to reduce sick-leave due to low-back disorders by intervention measures directed toward both the work (environment, tools) and the workers (working methods, positive attitude to work, preparedness to keep fit). (4) The identification of ergonomic hazards with the help of teamwork proved to be feasible. The practical improvements, however, were difficult to implement in the prevailing conditions. Some of the improvements increased the work load and the stress of the workers. This aspect needs further consideration. 相似文献
3.
我省的新一代天气雷达在多年的运行过程中出现了各种不同类型的故障,有些严重故障直接导致雷达停机,影响了气象预报服务.本文介绍和总结了该雷达发射机部分出现的一些主要故障,这些故障都集中在雷达射频驱动器、聚焦线圈电源、触发器、调制器、后充电调整器等组件上.通过对这些组件的功能和工作原理的分析,详细介绍发射机故障诊断分析的关键技术. 相似文献
4.
针对基于错误学习(LWE)问题的CKKS同态加密方案在密态数据计算中存在的密文大、计算密钥生成复杂以及同态计算效率低的缺陷,运用比特丢弃和同态计算密钥重组的方法,提出了一种优化的LWE型CKKS方案。首先,丢弃密文向量的部分低位比特和同态乘法计算中密文张量积的部分低位比特,从而减小了同态乘法过程中的密文规模;其次,针对比特丢弃对同态计算密钥进行重组和优化,从而去除密钥交换过程中powersof2含有的无关扩展项并降低计算密钥的规模和同态乘法过程中的噪声增长规模。在保证原有方案安全性的基础上,所提优化方案使得计算密钥的维度减少,使得同态乘法的计算复杂性降低。分析结果表明,所提出的优化方案在一定程度上降低了同态计算及计算密钥生成过程的计算复杂性,从而降低了存储开销并提升了同态乘法运算的效率。 相似文献
5.
6.
介绍企业在发展中存在的八大误区为:(1)企业越大越好;(2)发展越快越好;(3)集体决策优于个人决策;(4)多元化经营可降低风险;(5)什么赚钱干什么;(6)成功带来更大的成功;(7)好产品代表一切;(8)从产品经营到资本运营. 相似文献
7.
Markus Kostrzewa Katrin Sparbier Thomas Maier Sören Schubert 《Proteomics. Clinical applications》2013,7(11-12):767-778
MALDI-TOF MS profiling for microorganism detection has already been demonstrated in the 1990s, but has evolved to the first-line identification method in many laboratories just during the past five years. While this application of MALDI-TOF MS has proven its broad applicability, accuracy, robustness, and cost-effectiveness it is of particular interest to expand the capabilities of the mass spectrometric platform. Resistance detection is the most desirable further application of MALDI-TOF MS in microbiology, but maybe also the most challenging. Different approaches have been published regarding diverse antibiotic drugs and distinct microorganism classes. The current review shall give an overview about the developments of the recent years and their potential to get transformed in clinical useful assays in the future. 相似文献
8.
Suboptimal robust synthesis for MIMO nominal system under coprime factor perturbations is considered in classical and non-classical statements. In the classical statement, weights of perturbations and upper bound on magnitude bounded exogenous disturbance are assumed to be known to controller designer. Suboptimal synthesis within ε tolerance is reduced to the solution of log2(1/ε) standard mixed sensitivity problems of ℓ1 optimization. In the non-classical statement, the upper bounds on perturbations and exogenous disturbance are to be estimated from measurement data and suboptimal synthesis is reduced to the solution of 1/ε mixed sensitivity problems. 相似文献
9.
Szu-Yuan Jhen-Gang Tsung-Liang Jin-Chuan Yi-Kuang Mark Deirdre R. Chun-Nan Chii-Wann 《Sensors and actuators. B, Chemical》2008,133(2):493-501
We recently reported the successful use of the loop-mediated isothermal amplification (LAMP) reaction for hepatitis B virus (HBV) DNA amplification and its optimal primer design method. In this study, we report the development of an integrated isothermal device for both amplification and detection of targeted HBV DNA. It has two major components, a disposable polymethyl methacrylate (PMMA) micro-reactor and a temperature-regulated optical detection unit (base apparatus) for real-time monitoring of the turbidity changes due to the precipitation of DNA amplification by-product, magnesium pyrophosphate. We have established a correlation curve (R2 = 0.99) between the concentration of pyrophosphate ions and the level of turbidity by using a simulated chemical reaction to evaluate the characteristics of our device. For the applications of rapid pathogens detection, we also have established a standard curve (R2 = 0.96) by using LAMP reaction with a standard template in our device. Moreover, we also have successfully used the device on seven clinical serum specimens where HBV DNA levels have been confirmed by real-time PCR. The result indicates that different amounts of HBV DNA can be successfully detected by using this device within 1 h. 相似文献
10.
Maria Kelly Venezuela Mônica Carneiro SandovalDenise Aparecida Botter 《Computational statistics & data analysis》2011,55(4):1867-1883
Local influence diagnostics based on estimating equations as the role of a gradient vector derived from any fit function are developed for repeated measures regression analysis. Our proposal generalizes tools used in other studies (
[Cook, 1986] and [Cadigan and Farrell, 2002]), considering herein local influence diagnostics for a statistical model where estimation involves an estimating equation in which all observations are not necessarily independent of each other. Moreover, the measures of local influence are illustrated with some simulated data sets to assess influential observations. Applications using real data are presented. 相似文献