Intimin及其受体Tir在致病性大肠杆菌致细胞线粒体功能障碍中的作用

目的探讨致病性大肠杆菌(Enteropathogenic Escherichia coli,EPEC)外膜蛋白Intimin及其受体Tir在EPEC致HeLa细胞线粒体功能障碍中的作用。方法将EPEC外膜蛋白Intimin及其受体Tir删除株、相应质粒互补株或染色体互补株感染HeLa细胞,用线粒体膜电位(Mitochondria membrane potential,MMP)检测试剂JC-1染色细胞线粒体,通过多功能酶标仪检测MMP水平,Western blot检测Intimin的表达及Tir的转位。结果与野生型菌株相比,Eae删除株和Tir删除株感染细胞的MMP功能显著减弱(P<0.05),Eae删除株功能能被质粒表达相应蛋白所互补,Tir删除株不能被质粒表达Tir互补,但可被染色质表达野生型Tir或TirY474S互补,而染色质TirS434A突变株不能引起明显的MMP下降。结论 Intimin和Tir是参与线粒体功能障碍的重要分子;TirS434在线粒体功能障碍中起重要作用。     

Modeling and analysis of strategic forward contracting in transmission constrained power markets

Taking the effects of transmission network into account, strategic forward contracting induced by the interaction of generation firms’ strategies in the spot and forward markets is investigated. A two-stage game model is proposed to describe generation firms’ strategic forward contracting and spot market competition. In the spot market, generation firms behave strategically by submitting bids at their nodes in a form of linear supply function (LSF) and there are arbitrageurs who buy and resell power at different nodes where price differences exceed the costs of transmission. The owner of the grid is assumed to ration limited transmission line capacity to maximize the value of the transmission services in the spot market. The Cournot-type competition is assumed for the strategic forward contract market. This two-stage model is formulated as an equilibrium problem with equilibrium constraints (EPEC); in which each firm's optimization problem in the forward market is a mathematical program with equilibrium constraints (MPEC) and parameter-dependent spot market equilibrium as the inner problem. A nonlinear complementarity method is employed to solve this EPEC model.     

The EcpD Tip Adhesin of the Escherichia coli Common Pilus Mediates Binding of Enteropathogenic E. coli to Extracellular Matrix Proteins

The attachment of enteropathogenic Escherichia coli (EPEC) to intestinal epithelial cells is facilitated by several adhesins; however, the individual host-cell receptors for pili-mediated adherence have not been fully characterized. In this study, we evaluated the hypothesis that the E. coli common pilus (ECP) tip adhesin protein EcpD mediates attachment of EPEC to several extracellular matrix (ECM) glycoproteins (fibronectin, laminin, collagens I and IV, and mucin). We found that the ΔecpA mutant, which lacks production of the EcpA filament but retains EcpD on the surface, adhered to these glycoproteins below the wild-type levels, while the ΔecpD mutant, which does not display EcpA or EcpD, bound significantly less to these host glycoproteins. In agreement, a purified recombinant EcpD subunit bound significantly more than EcpA to laminin, fibronectin, collagens I and IV, and mucin in a dose-dependent manner. These are compelling data that strongly suggest that ECP-producing EPEC may bind to host ECM glycoproteins and mucins through the tip adhesin protein EcpD. This study highlights the versatility of EPEC to bind to different host proteins and suggests that the interaction of ECP with the host’s ECM glycoproteins may facilitate colonization of the intestinal mucosal epithelium.     

基于CAN总线的铁路救援起重机控制系统设计

根据铁路救援起重机的控制要求,设计了基于CAN总线的铁路救援起重机控制系统.控制器采用EPEC OY开发的CAN控制系统模块.在控制模块之间、控制模块与起重机执行机构之间,采用CAN总线作为信息交换的通道.介绍了CANOpen协议的技术特点,给出了控制器节点的CANopen初始化流程.运用CoDeSyS 软件对控制器进行编程,实现对铁路救援起重机的控制.本控制系统具有检测、控制、报警功能,且操作简单,能极大地提高铁路救援起重机在作业时的安全性能,为铁路救援起重机在恶劣的环境下长期、稳定、高效的工作提供了保障.     

Map of Enteropathogenic Escherichia coli Targets Mitochondria and Triggers DRP-1-Mediated Mitochondrial Fission and Cell Apoptosis in Bovine Mastitis

Bovine mastitis seriously affects bovine health and dairy product quality. Escherichia coli is the most important pathogen in the environment and dairy products. Enteropathogenic Escherichia coli (EPEC) is a zoonotic pathogen, which seriously threatens the health of people and dairy cows. We recently reported that E. coli can induce endogenous apoptosis in bovine mammary epithelial cells. However, the mechanism of EPEC-damaged mitochondria and -induced bovine mastitis is unclear. In this study, we found that EPEC can induce DRP-1-dependent mitochondrial fission and apoptosis. This was verified by the application of Mdivi, a DRP-1 inhibitor. Meanwhile, in order to verify the role of the Map virulence factor in EPEC-induced bovine mastitis, we constructed a map mutant, complementary strain, and recombinant plasmid Map_His. In the present study, we find that Map induced DRP-1-mediated mitochondrial fission, resulting in mitochondrial dysfunction and apoptosis. These inferences were further verified in vivo by establishing a mouse mastitis model. After the map gene was knocked out, breast inflammation and apoptosis in mice were significantly alleviated. All results show that EPEC targets mitochondria by secreting the Map virulence factor to induce DRP-1-mediated mitochondrial fission, mitochondrial dysfunction, and endogenous apoptosis in bovine mastitis.     

基于EPEC控制器的采煤机控制系统的设计

针对基于PLC的采煤机控制系统存在防护等级低、抗振动能力差、结构布置复杂等问题,提出了一种基于EPEC控制器的采煤机控制系统的设计方案。该系统采用带CAN总线的EPEC控制器将现场实时数据打包成PDO报文传输至CAN总线,再由CAN232MB和CAN485MB模块分别将报文传输给显示器和上位机,从而实现对采煤机运行状态的实时监测。实际应用表明,该系统提高了采煤机控制系统的抗干扰性和可靠性,降低了采煤机控制系统的故障率。     

EPEC控制器在掘进机控制系统的研究

将Code Sys软件平台引入掘进机控制领域,结合CANopen现场总线协议,设计出了一种先进的掘进机控制系统,硬件采用EPEC控制器,在Co De Sys平台上完成了对掘进机操作箱的通讯、掘进机的动作控制、保护以及状态显示。     

IBMPC与可编程控制器EPEC2023、2024主从式测控系统的研制

本文介绍了上位机———IBMPC与下位机———可编程控制器EPEC2 0 2 3、2 0 2 4所构成的主从式测控系统 ,主要阐述了系统的总体设计方案 ,包括下位机控制模块、上位机监控模块以及上位机和下位机的通信模块的硬软件设计 ,重点是系统中实现了控制局域网CAN总线与PC之间的通信 ,使CAN总线上的数据能够在PC上可视化并受到监控     

肠致病性大肠杆菌适配体筛选研究

目的食源性致病菌引起的食源性疾病已成为全球食品安全面临的巨大威胁,其中由大肠杆菌引起的食源性疾病最为常见。因此发展快速、准确检测大肠杆菌的方法对于保障食品安全、保护国民健康具有重要意义。方法应用Whole bacteria-SELEX(指数富集的配体系统进化技术),以肠致病性大肠杆菌为靶标,经过15轮的筛选富集,将所得产物进行克隆测序,并结合荧光分析考察所得序列的亲和力和特异性,根据解离常数值分析比较序列的亲和力和特异性,最终得到2条(Seq.1和Seq.28)与肠致病性大肠杆菌高特异性结合的适配体。结果适配体Seq.1和Seq.28均对肠致病性大肠杆菌表现出了相对良好的亲和力和特异性(对其他菌的相对亲和力均低于15%),解离常数分别为45.06±6.797和32.31±6.002 nmol/L。结论本文利用SELEX技术筛选特异性识别肠致病性大肠杆菌适配体,具有稳定性高、合成方便、易标记等特点,可进一步应用于食品中肠致病性大肠杆菌的快速检测。