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1.
An NP-hard production–distribution problem for one product over a multi-period horizon is investigated. The aim is to minimize total cost taking production setups, inventory levels and distribution into account. An integer linear model is proposed as a compact problem specification but it cannot be solved to optimality for large instances. Instead of using a classical two-phase approach (production planning and then route construction for each day), metaheuristics that simultaneously tackle production and routing decisions are developed: a GRASP (greedy randomized adaptive search procedure) and two improved versions using either a reactive mechanism or a path-relinking process. These algorithms are evaluated on 90 randomly generated instances with 50, 100 and 200 customers and 20 periods. The results confirm the interest of integrating production and distribution decisions, compared to classical two-phase methods. Moreover, reaction and path-relinking give better results than the GRASP alone. 相似文献
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LM331是一种精密频率电压转换器。非常适用于用作模/数转换器。文中介绍了LM331的主要性能指标.同时给出了其与单片机8098的接口。 相似文献
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331省道盱眙绕城段改扩建工程施工直接影响到梁项水库蓄水、防洪与大坝堤身安全,本文分步骤祥述了梁项水库施工占用水库库容及附属工程补偿技术要点和解决方案。 相似文献
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MicroRNA-331-3p Suppresses Cervical Cancer Cell Proliferation and E6/E7 Expression by Targeting NRP2
Tomomi Fujii Keiji Shimada Aya Asano Yoshihiro Tatsumi Naoko Yamaguchi Masaharu Yamazaki Noboru Konishi 《International journal of molecular sciences》2016,17(8)
Aberrant expression of microRNAs (miRNAs) is involved in the development and progression of various types of cancers. In this study, we investigated the role of miR-331-3p in cell proliferation and the expression of keratinocyte differentiation markers of uterine cervical cancer cells. Moreover, we evaluated whether neuropilin 2 (NRP2) are putative target molecules that regulate the human papillomavirus (HPV) related oncoproteins E6 and E7. Cell proliferation in the human cervical cancer cell lines SKG-II, HCS-2, and HeLa was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay. Cellular apoptosis was measured using the TdT-mediated dUTP nick end labeling (TUNEL) and Annexin V assays. Quantitative RT-PCR was used to measure the messenger RNA (mRNA) expression of the NRP2, E6, E7, p63, and involucrin (IVL) genes. A functional assay for cell growth was performed using cell cycle analyses. Overexpression of miR-331-3p inhibited cell proliferation, and induced G2/M phase arrest and apoptosis in SKG-II, HCS-2 and HeLa cells. The luciferase reporter assay of the NRP2 3′-untranslated region revealed the direct regulation of NRP2 by miR-331-3p. Gene expression analyses using quantitative RT-PCR in SKG-II, HCS-2, and HeLa cells overexpressing miR-331-3p or suppressing NRP2 revealed down-regulation of E6, E7, and p63 mRNA and up-regulation of IVL mRNA. Moreover, miR-331-3p overexpression was suppressed NRP2 expression in protein level. We showed that miR-331-3p and NRP2 were key effectors of cell proliferation by regulating the cell cycle, apoptosis. NRP-2 also regulates the expression of E6/E7 and keratinocyte differentiation markers. Our findings suggest that miR-331-3p has an important role in regulating cervical cancer cell proliferation, and that miR-331-3p may contribute to keratinocyte differentiation through NRP2 suppression. miR-331-3p and NRP2 may contribute to anti-cancer effects. 相似文献
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基于LM331和单片机的数据采集控制 总被引:2,自引:0,他引:2
以LM331型V/F变换器为例,叙述了如何把采集的电压信号通过LM331V/F转换器精确变换成频率信号送与单片机实现数据采集控制的方法。详细介绍了系统各部分的构成、原理及用C语言进行测量控制的编程方法。 相似文献
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Conditions of synthesis for PpIX 总被引:1,自引:0,他引:1
Photodynamic therapy (PDT) is an effective treat-ment for cancer[1-3].In PDT treatment ,a tumor-locali-zing photosensitizer is administered,and sti mulated bylight with special wavelengthtoinitiate a chemical reac-tionleadingto the death of tumor cells .I… 相似文献
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数字脉宽调制方法的研究 总被引:1,自引:0,他引:1
杜坤梅 《哈尔滨理工大学学报》2001,6(2):18-22
借鉴集成DSP电机控制器ADMC331及其PWM控制器结构,对单边和双边数字脉宽调制方式进行了比较,提出了脉宽调制方式选择的基本原则,以三相交流电机电压型逆变器做实验研究,得出了相应的实验结果。 相似文献
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