乳酸乳球菌NICE系统食品级载体pRNB48的构建

乳酸菌食品级NICE系统是目前较理想的可控制蛋白质生产的食品级诱导表达系统。实验构建的食品级表达载体pRNB48含α-aga食品级选择标记、θ型复制子和nisA启动子,与宿主菌L.lactisNZ9000共同构成乳酸乳球菌食品级NICE系统,可用于目的基因在乳酸乳球菌中高效诱导表达。     

应用层多播协议NICE及其在移动无线网络中的应用

随着Internet的发展,在诸如音频和视频流等多种的应用中多播技术显得日益重要,然而传统的IP多播技术却不能满足ISP的要求.介绍了应用层多播的概念及其特点,讨论并分析了一种应用层多播协议NICE.越来越多的多播应用是面向移动无线网络的,移动无线网络是一种无基础设施的拓扑结构可变的网络,NICE能有效、强健地解决移动无线网络中的多播问题.     

Assumed strain nodally integrated hexahedral finite element formulation for elastoplastic applications

In this work, a linear hexahedral element based on an assumed strain finite element technique is presented for the solution of plasticity problems. The element stems from the Nodally Integrated Continuum Element (NICE) formulation and its extensions. Assumed gradient operators are derived via nodal integration from the kinematic‐weighted residual; the degrees of freedom are only the displacements at the nodes. The adopted constitutive model is the classical associative von Mises plasticity model with isotropic and kinematic hardening; in particular, a double‐step midpoint integration algorithm is adopted for the integration and solution of the relevant nonlinear evolution equations. Efficiency of the proposed method is assessed through simple benchmark problems and comparison with reference solutions. Copyright © 2014 John Wiley & Sons, Ltd.     

自相似结构化覆盖网组播模拟器设计

针对当前流行覆盖网组播模拟器无法准确为组播系统建模及评测性能的问题,提出一种节点群支持整体代换和多树结构的自相似结构化覆盖网组播(S3M)模型的,设计一个结构化覆盖网组播模拟器,在简化S3M模型结构的基础上为其建立合适的仿真模型。与NICE协议的对比表明,该结构化覆盖网组播模型是有效的。     

一种分层分簇的应用层组播方案

在对NICE应用层组播协议研究的基础上,提出了一种新的应用层组播方案LCcast。它采取分层分簇的结构特征,从簇中选择出能力强的领导节点用Chord环组织起来。在数据传输方面,使用Chord环中定义的指向表并结合Dijkstra算法生成最小延迟的组播树,从而减少了传输延迟。同时,为了防止数据包丢失,对每一个簇选择了一个备用领导节点集合。仿真结果表明,LCcast组播方案在一定程度上减少了时间延迟,提高了平均数据传输率,降低了控制开销和领导节点的负载。     

Metasin—An Intra-Operative RT-qPCR Assay to Detect Metastatic Breast Cancer in Sentinel Lymph Nodes

Nodal status is one of the most important prognostic factors in breast cancer. Established tests such as touch imprint cytology and frozen sections currently used in the intra-operative setting show variations in sensitivity and specificity. This limitation has led to the development of molecular alternatives, such as GeneSearch, a commercial intra-operative real-time quantitative Polymerase Chain Reaction (RT-qPCR) assay that allows the surgeon to carry out axillary clearance as a one-step process. Since GeneSearch has been discontinued, we have developed the replacement Metasin assay, which targets the breast epithelial cell markers CK19 and mammaglobin mRNA and identifies metastatic disease in sentinel lymph nodes. The optimised assay can be completed within 32 min (6 min for RNA preparation and 26 min instrument run time), making its use feasible in the intraoperative setting. An analysis by Metasin of 154 archived lymph node homogenates previously analysed by both parallel histology and GeneSearch showed concordance for 148 cases. The sensitivity and specificity of Metasin compared with GeneSearch were 95% (CI 83%–99%) and 97% (CI 91%–99%) respectively; compared with histology they were 95% (CI 83%–99%) and 97% (CI 91%–99%), respectively. The sensitivity and specificity of GeneSearch compared with histology were 90% (CI 77%–96%) and 97% (CI 93%–99%) respectively. The positive predictive value of Metasin was 90% and negative predictive value was 98% for both histology and GeneSearch. The positive predictive value of GeneSearch was 92% and the negative predictive value was 97% compared to histology. The discordance rates of Metasin with both GeneSearch and histology were 3.89%. In comparison, the discordance rate of GeneSearch with histology was 4.5%. Metasin’s robustness was independently evaluated on 193 samples previously analysed by GeneSearch from the Jules Bordet Institute, where Metasin yielded comparable results.     

ENICE:考虑节点异构性的应用层组播协议

提出了一种解决节点异构性问题的方法,并将该方法应用于NICE协议的领导节点选择,提出了改进协议ENICE.通过理论推导和实验分析,证明该协议在整网stress和stretch方面,与NICE协议基本类似,在平均传输延迟和最大延时方面,比NICE协议要优越.     

中国纸产品及其能源需求和CO2排放的情景分析（Ⅰ）

根据NICE模型结构,对中国纸产品需求进行了情景分析。发现消费人口、城市化率是中国纸产品的两个基本变量;对于包装用纸,采用能源需求作为变量进行回归分析．发现了非常强的相关关系。分析结果表明,中国未来纸品需求量到2055年左右将基本达到稳定,人均消费量超过100千克大关,将迟到130千克左右。     

乳酸乳球菌两组分食品级NICE系统载体的构建

乳酸菌食品级NICE系统是目前较理想的可控制蛋白质生产的食品级诱导表达系统。本实验构建的食品级表达载体pRNA48含α-aga食品级选择标记、θ型复制子和nisA启动子，与宿主菌L．hctis NZ9000共同构成乳酸乳球菌食品级NICE系统，可用于目的基因在乳酸乳球菌中高效诱导表达。