Modeling of protein adsorption to DEAE sepharose FF: Comparison of data with model simulation

The equilibrium and kinetic characteristics of the adsorption of human serum albumin (HSA) and ovalbumin (OVA) to the DEAE Sepharose FF weak anion exchanger were experimentally determined. The rate for protein adsorption was simulated with two different models, the first being based on a single lumped kinetic parameter, while the second includes the individual mass transfer processes occurring prior to the adsorption intervention, i.e., diffusion across the liquid film surrounding individual particles and diffusion within the ion exchanger particle itself. The actual adsorption of OVA to DEAE Sepharose FF in fully mixed stirred vessels and in packed bed columns was consistent with both models. In the case of HSA, however, the adsorption profile in an agitated vessel was consistent only with the pore diffusion model and neither model could correctly predict the latter part of the breakthrough profile observed in packed bed experiments.     

A reliable and sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) for ochratoxin A in agro-products

Immunochromatographic assays (ICAs) are considered as a suitable diagnostic tool for the detection of mycotoxins. Mycotoxins and especially, ochratoxin A are analytes with more demanding sensitivity requirements. To enhance the sensitivity of current immunochromatographic assays for ochratoxin A (OTA), a novel sensitive ICA was developed in this study. In the assay, microspheres enclosing fluorescent europium (III) [Eu(III)] nanoparticles (EuNPs) were used as a label for OTA monoclonal antibody (OTA-mAb) conjugation. Accordingly, assay was called time-resolved fluorescent immunochromatographic assay (TRFICA). The test strip was composed of three parts: a sample pad, nitrocellulose membrane and an absorbent pad. As for detection, a proper concentration of conjugated microspheres was pipetted into the microtube and sample extract was added to it. Then the strip was inserted into the tube and the fluid flow along the strip. The TRFICA results were obtained in 8 min and read by a portable TRFICA strip reader. The established method allows quantitative determination of OTA with limit of detection as low as 1.0 μg kg⁻¹ in the samples. For validation, spiked samples including wheat, maize, soybean and rice were respectively assayed by TRFICA and a standard high performance liquid chromatography equipped with a fluorescence detector (HPLC-FLD), and good agreement of results was obtained between two methods.     

SM_2人工抗原合成研究

采用重氮化法合成磺胺二甲嘧啶(SM2)-人血清白蛋白(HAS)免疫抗原和SM2-卵清白蛋白(OVA)包被抗原。经紫外光谱扫描法确认SM2与载体蛋白偶联成功;经计算得SM2与HSA、OVA的结合比分别为9:1和15:1。     

断言技术在SOC验证中的应用

主要阐述了基于断言技术新硬件验证平台用于实时监测设计描述是否违反了设计目标,提出了OVA的特点及应用,并以LCD controller的sharp接口时序为实例讲解了该方法的优点。     

The impact of transgenic papaya (TPY10-4) fruit supplementation on immune responses in ovalbumin-sensitised mice

BACKGROUND: A transgenic papaya line (TPY10‐4) that is resistant to both papaya ringspot virus (PRSV) and papaya leaf distortion mosaic virus (PLDMV) has been developed in Taiwan. This study investigated the immunomodulatory properties of transgenic TPY10‐4 and its native (TCK) papaya fruits using an ovalbumin (OVA)‐sensitised mouse model. Both green and ripe papaya fruits at low (0.2 g powder kg^?1 body weight (BW)) and high (1.6 g powder kg^?1 BW) doses were administered to experimental mice by intragastric gavage for 5 weeks. Changes in serum total immunoglobulin A (IgA), IgE, IgG and IgM levels, OVA‐specific IgE, IgG1 and IgG2a titres and Th1/Th2 cytokine secretions using splenocytes were determined. RESULTS: Transgenic TPY10‐4 or native TCK papaya fruit supplementation did not significantly affect body, visceral organ and relative tissue weights, total IgE antibody levels, OVA‐specific IgE and IgG1 antibody titres or OVA‐stimulated interferon‐γ (IFN‐γ), interleukin‐2 (IL‐2), IL‐4, IL‐5 and IL‐10 secretions using splenocytes. However, transgenic papaya fruits markedly increased serum total IgM levels. CONCLUSION: This study suggests that transgenic TPY10‐4 papaya fruits do not increase the allergenic potential of OVA by oral administration but may have a protective immunity via increasing the serum total IgM level. Copyright © 2010 Society of Chemical Industry     

卵清蛋白-PLGA抗原微球脉冲释放系统的研究

目的：以OVA为模型抗原研制具有脉冲释放特性的OVA-PLGA微球并对其在小鼠体内的免疫效果进行评价。方法：复乳法研制OVA-PLGA微球;光学显微镜和电子显微镜法研究微球的形态、粒径和粒径分布;Bradford法测定微球的载药量和包封率;微量溶出度测定法研究微球的体外释放规律;按注射剂的要求研制含OVA-PLGA微球...     

忍冬水提物对卵清蛋白致敏小鼠的疗效

目的研究忍冬水提物对卵清蛋白(Ovalbumin,OVA)致敏小鼠的疗效。方法建立OVA致敏小鼠模型,将OVA致敏BALB/c小鼠随机分为5组,即忍冬水提物高浓度组(H组)、中浓度组(M组)、低浓度组(L组)、模型对照组(CH组)及阴性对照组(NS组);采用小鼠足垫肿胀试验检测迟发型超敏反应;甲苯胺蓝及HE染色观察小鼠肠系膜、空肠肥大细胞及空肠绒毛上皮细胞形态;ELISA法检测小鼠血清OVA特异性IgE及IgG1、小肠黏液总IgA及OVA特异性IgA水平。结果 OVA致敏小鼠出现急性腹泻;致敏小鼠经足垫皮内激发,出现迟发型超敏反应;致敏小鼠肠系膜及空肠肥大细胞聚集、胞质颗粒外泄及空肠绒毛炎症细胞浸润;小鼠血清OVA特异性IgE及小肠黏液OVA特异性IgA水平明显升高(P均<0.01)。经H、M组忍冬水提物灌胃后致敏小鼠的一般状态、腹泻情况及空肠炎症明显缓解;OVA介导的足垫肿胀反应明显减弱;小鼠血清OVA特异性IgE及小肠黏液OVA特异性IgA水平明显降低(P均<0.01)。结论一定浓度忍冬水提物可有效缓解OVA致敏小鼠的速发型与迟发型超敏反应,其中高浓度金银花忍冬水提物效果较好。提示忍冬科植物中某些成分具有抗过敏作用。     

Hybrid algorithmic approach oriented to incipient rotor fault diagnosis on induction motors

This paper investigates the current monitoring for effective fault diagnosis in induction motor (IM) by using random forest (RF) algorithms. A rotor bar breakage of IM does not derive in a catastrophic fault but its timely detection can avoid catastrophic consequences in the stator or prevent malfunctioning of those applications in which this sort of fault is the primary concern. Current-based fault signatures depend enormously on the IM power source and in the load connected to the motor. Hence, homogeneous sets of current signals were acquired through multiple experiments at particular loading torques and IM feedings from an experimental test bench in which incipient rotor severities were considered. Understanding the importance of each fault signature in relation to its diagnosis performance is an interesting matter. To this end, we propose a hybrid approach based on Simulated Annealing algorithm to conduct a global search over the computed feature set for feature selection purposes, which reduce the computational requirements of the diagnosis tool. Then, a novel Oblique RF classifier is used to build multivariate trees, which explicitly learn optimal split directions at internal nodes through penalized Ridge regression. This algorithm has been compared with other state-of-the-art classifiers through careful evaluation of performance measures not encountered in this field.     

Using OVA modeling to improve classification performance for large datasets

One-Versus-All (OVA) classification is a classifier construction method where a k-class prediction task is decomposed into k 2-class sub-problems. One base model is constructed for each sub-problem and the base models are then combined into one model. Aggregate model implementation is the process of constructing several base models which are then combined into a single model for prediction. In essence, OVA classification is a method of aggregate modeling. This paper reports studies that were conducted to establish whether OVA classification can provide predictive performance gains when large volumes of data are available for modeling as is commonly the case in data mining. It is demonstrated in this paper that firstly, OVA modeling can be used to increase the amount of training data while at the same time using base model training sets whose size is much smaller than the total amount of available training data. Secondly, OVA models created from large datasets provide a higher level of predictive performance compared to single k-class models. Thirdly, the use of boosted OVA base models can provide higher predictive performance compared to un-boosted OVA base models. Fourthly, when the combination algorithm for base model predictions is able to resolve tied predictions, the resulting aggregate models provide a higher level of predictive performance.