Simple Sequence Repeat Markers in Genetic Divergence and Marker-Assisted Selection of Rice Cultivars: A Review

Sequencing of rice genome has facilitated the understanding of rice evolution and has been utilized extensively for mining of DNA markers to facilitate marker-assisted breeding. Simple sequence repeat (SSR) markers that are tandemly repeated nucleotide sequence motifs flanked by unique sequences are presently the maker of choice in rice improvement due to their abundance, co-dominant inheritance, high levels of allelic diversity, and simple reproducible assay. The current level of genome coverage by SSR markers in rice is sufficient to employ them for genotype identification and marker-assisted selection in breeding for mapping of genes and quantitative trait loci analysis. This review provides comprehensive information on the mapping and applications of SSR markers in investigation of rice cultivars to study their genetic divergence and marker-assisted selection of important agronomic traits.     

珠江口门海堤堤型的选择

珠江口站地区面临南海、大陆岸线长４５７ｋｍ，遭受洪水。潮汐、台风的影响，河口滩涂 发育迅速，以约１００ｍ／ａ的速度向大海延伸。随着沿海地区的经济发展，滨海－２ｍ高程以上的滩涂的４万ｈｍ＾２正在和将要建堤围垦和开发利用，原有海堤也亟待加固提高，因此，对珠江口门坝有海堤堤型资料进行收集、整理，分析总结，选择经济可行的海堤堤型，具有重要的现实意义。文中收集了珠江口门的几种主要堤型，通过对各种堤基工程地质     

珠江三角洲土坝和堤围震害初步预测

本项预测采用概率方法,即首先根据广东省水利部门提出的55座土坝和59个重点的座标(见附图),进行概率地震危险性分析,给出它们在一定预测年限T(取50年和100年)的地震烈度Ⅰ的超越概率;其次根据近年我国发生的几次强震时处于不同烈度区内的土坝和堤防的震害资料,形成表示土坝和土堤地震易损性的震害概率矩阵;然后,根据上述两方面结果用概率公式评价各土坝和堤围点在预测年限内各震害等级的超越概率,并取10％超越概率为标准,估计震害等级。结果表明,珠江三角洲土工构筑物的地震危害性南部高于北部。     

珠江口盆地(西部)早第三纪古环境分析

珠江口盆地(西部)早第三纪的古环境变迁与盆地的形成及演化紧密相关。早第三纪盆地演化经历了4个阶段。第一阶段,盆地早期拉张,以粗粒沉积物沉积为主,部分地域发育小湖盆。第二阶段,盆地稳定下沉,是富营养性湖泊的鼎盛期,形成优质湖相烃源岩。第三阶段,湖盆萎缩,煤系沼泽发育,形成湖相和煤系两类重要烃源岩。第四阶段,古湖泊消亡,海水漫进,滨浅海砂岩体发育,形成油气勘探的有利目的层。     

The distribution of anthocyanins in grape skin extracts of port wine cultivars as determined by high performance liquid chromatography

The distribution of anthocyanins in methanolic skin extracts of 16 grape cultivars used for port wine production and grown at five sites in the Douro Valley in Northern Portugal has been assessed by high performance liquid chromatography. Results are confined to the seven most readily separated and identified anthocyanins and are expressed as percentages of their total (88–99% of the total integrated area). Anthocyanins based on malvidin (Mv) predominated. Of these, Mv 3-glucoside was the major pigment (33–60%), being exceeded by Mv 3-p-coumarylglucoside (2–51%) in only two cultivars; Mv 3-acetylglucoside (1–15%) was consistently the lowest. Peonidin 3-glucoside (1–27%) was prominent in four cultivars, but delphinidin 3-glucoside (1–11%), petunidin 3-glucoside (2–11%) and cyanidin 3-glucoside (trace-6%) were of low proportions throughout. The ratio Mv 3-acetylglucoside/total Mv glucosides appeared characteristic of cultivar, independent of site, and a useful aid to identification of grape cultivars. The coloured pulp of some cultivars contained peonidin 3-glucoside as a major component, present in greater proportions than in the skin; the percentage of Mv 3-p-coumarylglucoside was lower in pulp than in skin. The identities of six of the seven anthocyanins were confirmed by fast atom bombardment mass spectrometry and the structures of the Mv derivatives were determined by nuclear magnetic resonance. The formation of formyl and acetyl anthocyanin artefacts is described.     

Phosphorus seed coating increases phosphorus uptake,early growth and yield of pearl millet (Pennisetum glaucum (L.) R. Br.) grown on an acid sandy soil in Niger,West Africa

In pot and field experiments conducted in 1990 and 1991 on an acid sandy, phosphorus (P) deficient soil in Niger, West Africa, the effect of seed coating on seedling emergence, early growth and grain yield of pearl millet (Pennisetum glaucum (L.) R. Br.) was studied. Seeds of pearl millet were coated with different rates (0; 0.5; 1.0; 2.0; 5.0; 10.0 mg P seed^–1) and types of P fertilizers (single superphosphate, ammonium dihydrogen phosphate; monocalcium phosphate, sodium dihydrogen phosphate and sodium triphosphate). Seedling emergence was generally reduced at coating rates higher than 0.5 mg P seed^–1 and prevented with single superphosphate and sodium triphosphate at rates higher than 5 mg P seed^–1. No correlation was found between the pH and osmomolity of the coatings and final emergence of millet seedlings. The most favourable effect on plant growth and P content was achieved with ammonium dihydrogen phosphate (AHP) as seed coating. This was attributed to the enhancement effect of ammonium on P uptake. Compared to the untreated control dry matter production at 20 days after planting (DAP) was increased by 280%, P content per plant by 330%, total biomass at maturity by 30% and grain yield by 45%. Although seed coating with AHP may be harmful to seedlings emergence, it represents a suitable method to enhance early growth and increase yield of pearl millet.     

珠江干流(2008—2015年)水质变化趋势分析

以珠江干流为研究区域,选取源头及干流10个水质断面的2008-2015年7项参数监测数据,采用季节性kendall趋势检验法分析珠江干流的水质变化趋势,得出珠江干流各测站的pH、溶解氧(DO)、高锰酸盐指数(CODMn)、总磷(TP)、氟化物(F-)共5项参数趋于改善,五日生化需氧量(BOD5)及氨氮(NH3-N)共2项参数浓度基本保持不变;流域水质变化综合指数WQTI表明珠江干流总体水质状况趋向改善;水质呈现上游恶化中下游好转、污染物呈现以省区为单位以省会为中心的西部低东部高的地域分布特点.     

基于2030可持续发展目标的珠三角土地消耗率与人口增长率协调关系评价

量化联合国可持续发展目标SDG 11.3.1“土地消耗率与人口增长率的比率（LCRPGR）”有助于了解城市扩张与人口增长之间的关系,为城市国土空间规划和人口城镇化调控提供数据支撑,并且对指导决策者制定城市增长计划至关重要。基于土地利用产品、夜间灯光数据和人口普查数据,提取了城市建成区,利用地理加权回归模型测算了珠三角地区1 km×1 km格网尺度人口密度。依据SDG 11.3.1指标元数据中定义和公式,对珠三角地区可持续发展进行了可靠评价。结果显示：①1990~2010年珠三角建成区面积扩张4.6倍,城区人口增长3.7倍;②1990~2010年珠三角的LCRPGR值由0.71增长到2.01;城市扩张速度与人口增长速度并非是成比例协调增长关系;③建成区的扩张主要是由耕地和农村居民用地转换而来。综上,自2000年后珠三角地区土地消耗率已经超过人口增长率,城市扩张速度与人口增长速度并非是成比例协调增长关系,二者的差异在增大,应当引起一定注意。     

咸潮影响下磨刀门水道取淡时机初探

对磨刀门水道2005-2010年枯水期实测数据进行分析,提出“最早取淡日”和“最后取淡日”的概念,探讨潮周期最早取淡日与最大潮差日、最后取淡日与最小潮差日关系.结果表明:在枯水期,相对于最大潮差日,最早取淡日呈逐月向后推移的趋势,压咸流量一般应在潮周期最早取淡日之前1d到达思贤滘;最后取 淡日与最小潮差日关系较复杂,...