Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.     

Protein-based nanomaterials and nanosystems for biomedical applications: A review

Advanced protein-based nanomaterials and nanosystems (PNNS) have attracted considerable scientific interest in recent decades due to their potential in bio-applications. Nowadays, the constructed PNNS exhibit different properties for various special applications based on the characteristics of different proteins. Herein, in this review article, a systematic summary and discussion focusing on designing multi-functional PNNS are presented. The latest developments in unique synthesis strategies and detailed classification of PNNS are reviewed. The functions of proteins in PNNS for biomedical applications, such as targeting proteins, carriers, enzymes, and fluorescent indicators, are summarized. Finally, the challenges and forward-looking perspectives of PNNS research are provided.     

芝麻营养原液的开发与研究

本文对芝麻营养保健成分的提取进行了系统的研究。确定了芝麻营养原液的制取工艺。为充分发挥芝麻的营养保健作用开辟了新的途径。     

特细砂混凝土的应用及其经济性

通过介绍特细砂混凝土在嘉陵江流域的马回、东西关、桐子壕等水电站建设中的应用情况 ,分析了特细砂混凝土的特性和配合比等特点 ,并闸明了在施工中使用特细砂混凝土的经济效益。     

Influence of protein and lipid concentration of the test lubricant on the wear of ultra high molecular weight polyethylene

To gain a better understanding of the ultra-high molecular weight polyethylene (UHMWPE) wear mechanism in the physiological environment, the effects of protein and lipid constituents of synovial fluid on the specific wear rate of UHMWPE were examined experimentally. The multidirectional sliding pin-on-plate wear tester was employed to simulate the simplified sliding condition of hip joint prostheses. Bovine serum γ-globulin and synthetic l--DPPC were used as model protein and lipid constituents of synovia, respectively. Results of the wear test indicated that the UHMWPE wear rate primarily depended on the protein concentration of the test lubricant. Lipids acted as a boundary lubricant and reduced polyethylene wear in the low protein lubricants. However, the polyethylene wear rate increased with increasing lipid concentrations if the protein concentration was within the physiological level. Increased interactions between protein and lipid molecules and lipid diffusion to polyethylene surface might be responsible for the increased wear.     

绿色再生混凝土利用途径与评定思路

就再生混凝土骨料进行深入系统的研究 ,建立了一套与现行评价天然骨料标准相一致的评定方法 ,具有工程实际意义 ,为将来实际应用作好了基础理论研究     

Immunoelectron localization of mitochondrial F1 factor in cryosections of heart muscle cells

Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A-gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor.     

Profile-Based Linguistic Uniformity as a Generic Method for Comparing Language Varieties

In this text we present``profile-based linguistic uniformity', a methoddesigned to compare language varieties on thebasis of a wide range of potentiallyheterogeneous linguistic variables. In manyrespects a parallel can be drawn with currentmethods in dialectometry (for an overview, see,Nerbonne and Heeringa, 2001; Heeringa, Nerbonneand Kleiweg, 2002): in both casesdissimilarities between varieties on the basisof individual variables are summarized inglobal dissimilarities, and a series oflanguage varieties are subsequently clusteredor charted using multivariate techniques suchas cluster analysis or multidimensionalscaling. This global similarity between themethods makes it possible to compare them andto investigate the implications of notabledifferences. In this text we specifically focuson, and defend one characteristic of ourmethodology, its profile-based nature.     

Influence of aggregate content on the migration coefficient of concrete materials using electrochemical method

In order to investigate the effect of coarse aggregate content on the chloride ion migration coefficient of concrete, specimens with different coarse aggregate volume fractions and two water/cement (w/c) ratios of mortar matrix were used. The chloride ion migration coefficient of concrete is obtained using the electrochemical technique to accelerate chloride ion migration in cement-based material and the experimental results were plotted as a function of the fine aggregate volume fraction. The results are analyzed comparing experimental results and theoretical models that represent the concretes as three-phase composite materials. The three phases are the mortar matrix, the coarse aggregate, and the interfacial transition zone between the two. The chloride ion migration coefficient is used to assess the dilution, tortuosity, interfacial transition zone (ITZ) and, percolation effects of coarse aggregate in concrete. It appears that the dilution and tortuosity effects are a dominant factor affecting the chloride ion migration coefficient of concrete in the low volume fraction of coarse aggregate. As the volume fraction of coarse aggregate increases to 40 and 20% in concrete of w/c ratio 0.35 and 0.45, respectively, the ITZ with percolation effects are significantly.     

海水冻融后轻骨料混凝土的双轴压压强度和变形性能

针对我国渤海湾北部海域轻骨料混凝土结构的工作环境和受力状态，进行了海水中不同冻融循环次数后轻骨料混凝土的双轴压压强度试验，考察了试件的破坏形态和表面裂缝的走向特征。根据试验结果，分析了极限抗压强度随冻融循环次数和应力比的变化规律，并在此基础上建立了考虑海水冻融影响的双轴压压强度准则。探讨了冻融循环作用后轻骨料混凝土的双轴压压变形性能，包括峰值应变、应力-应变关系随冻融循环次数和应力比的变化规律。研究结果说明，随冻融循环次数的增加各种应力比下的应力-应变关系曲线均逐渐扁平，峰值应力点明显下降并右移，说明混凝土的极限抗压强度逐渐降低。