Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.     

Protein-based nanomaterials and nanosystems for biomedical applications: A review

Advanced protein-based nanomaterials and nanosystems (PNNS) have attracted considerable scientific interest in recent decades due to their potential in bio-applications. Nowadays, the constructed PNNS exhibit different properties for various special applications based on the characteristics of different proteins. Herein, in this review article, a systematic summary and discussion focusing on designing multi-functional PNNS are presented. The latest developments in unique synthesis strategies and detailed classification of PNNS are reviewed. The functions of proteins in PNNS for biomedical applications, such as targeting proteins, carriers, enzymes, and fluorescent indicators, are summarized. Finally, the challenges and forward-looking perspectives of PNNS research are provided.     

Computerized IR Determination of Structural Group Composition for High-Boiling Oil Fractions

A computerized form is given of IR spectral determination for structural group composition in high-boiling oil fractions by Berthold's method (7 structural elements) and Kuklinskii's method (28 structural elements), which reduces the analysis time by factors of 3–4 and improves the accuracy.     

芝麻营养原液的开发与研究

本文对芝麻营养保健成分的提取进行了系统的研究。确定了芝麻营养原液的制取工艺。为充分发挥芝麻的营养保健作用开辟了新的途径。     

Analysis of polar compound classes in SRC-II liquids: Comparison of non-aqueous titrametric,i.r. spectrometric and h.p.l.c. methods

Results for various types of polar compounds in SRC-11 coal-derived liquids and other fuels were obtained by one or more of the three analytical methods: non-aqueous titration, i.r. spectroscopy and h.p.l.c. Practical aspects as well as precision, accuracy and assumptions necessary for effective application of each of the methods are discussed. H.p.l.c. is applicable to the widest variety of compound types, is the most rapid, most sensitive and shows the best promise for increased development and improvement. Application and development of these techniques is a logical step toward improving process monitoring, catalyst development, toxicological screening and general fuel analysis.     

Influence of protein and lipid concentration of the test lubricant on the wear of ultra high molecular weight polyethylene

To gain a better understanding of the ultra-high molecular weight polyethylene (UHMWPE) wear mechanism in the physiological environment, the effects of protein and lipid constituents of synovial fluid on the specific wear rate of UHMWPE were examined experimentally. The multidirectional sliding pin-on-plate wear tester was employed to simulate the simplified sliding condition of hip joint prostheses. Bovine serum γ-globulin and synthetic l--DPPC were used as model protein and lipid constituents of synovia, respectively. Results of the wear test indicated that the UHMWPE wear rate primarily depended on the protein concentration of the test lubricant. Lipids acted as a boundary lubricant and reduced polyethylene wear in the low protein lubricants. However, the polyethylene wear rate increased with increasing lipid concentrations if the protein concentration was within the physiological level. Increased interactions between protein and lipid molecules and lipid diffusion to polyethylene surface might be responsible for the increased wear.     

南京老虎山黄土-古土壤剖面不同粒级组分的磁化率及其古气候意义

利用沉降离心法将老虎山黄土-古土壤的酸不溶相分离成<2μm、2～45μm和>45μm三个粒级组分,并分别测定了这些组分和全岩的磁化率。结果表明,无论是全岩,还是三个不同粒级组分,它们的磁化率都可以单独作为长江中下游地区古气候演变的参考指标。其中,<2μm粒级的质量磁化率明显大于全岩和2～45μm、>45μm两粒级,而且其分辨率也明显高于2～45μm和>45μm这两个粒级组分。本实验结果还显示,虽然<2μum的质量磁化率最大,但对全岩质量磁化率的贡献率与2～45μm粒级相当,>45μm粒级的贡献率较低。     

Immunoelectron localization of mitochondrial F1 factor in cryosections of heart muscle cells

Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A-gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor.     

DT型粉状炸药专用蜡的研制

以多种含蜡馏分为基础原料，添加适量DT复合添加剂研制的DT型粉状炸药专用蜡具有优异的分散性和抗水性，可满足各种低梯粉状炸药要求，简化炸药配方，方便操作及其质量控制，以其生产的炸药爆速高，贮存期长，各项性能满足GB12437－2000标准要求。     

用凯氏定氮仪测定蛋白质结果的不确定度评定

本文列出影响用凯氏定氮仪测定蛋白质结果的因子,依据测量不确定度评定规范,尝试对测定蛋白质结果不确定度的评价。