Thermospray-LC-MS analysis of various groups of polyphenols in tea

An account is given of the application of thermospray LC-MS in the analysis of caffeoyl- andp-coumaroylquinic acids, theaflavins and thearubigins in black tea. All compounds, except for the thearubigens, could be detected as the pseudo-molecular ion [M+H]⁺. In addition to [M+H]⁺, other species such as adducts with sodium, ammonium and potassium, as well as solvent clusters were observed. The formation of those adducts depended upon on the structure of the compound. A fragmentation of chlorogenic acids occurred at elevated temperatures yielding the constituents of the molecules (caffeic,p-coumaric and quinic acids).     

Biochemical quality characteristics of CTC black teas of south India and their relation to organoleptic evaluation

A study was conducted over a period of 5 years to quantify the quality characteristics of CTC (crush, tear and curl) black teas produced in Wayanad, Central Travancore, Anamallais, High Range, Nilgiris and Chikmagalur areas of south India and their correlation with organoleptic evaluation. Quality characteristics such as theaflavins (TF), thearubigins (TR), highly polymerised substances (HPS), total liquor colour (TLC), TR/TF ratio and colour index (CI) were estimated. Teas from Karnataka, Nilgiris and Anamallais had the highest theaflavin content and better TR/TF ratio than the teas from Travancore, High Range and Wayanad regions. A significant positive correlation of theaflavin content with the values of thearubigins, total liquor colour, colour index, infused leaf appearance (ILA) and taste (TAS) was observed.     

Phenolic compounds in tea from Australian supermarkets

Phenolic compounds constitute 50–70% of tea water extract and are the main quality parameters for teas. Theaflavins (TF), thearubigins (TR) and theabrownins (TB) are the major polyphenols that determine the quality of black tea. These compounds were measured in 56 leaf teas and teabags sampled from Australian supermarkets in Queensland. The various quantities of TF, ranging from 0.29% to 1.25%, indicate a quality difference that exists among the teas studied. Low TF content in black tea may be due to over-fermenting and/or long periods of storage. The solubility of TR and TB from teabags ranged from 82% to 92%, indicating that the permeability of teabags was variable. Variable quantities of TF in Australian teas show instability and a tendency of TF to oxidize during storage. Total polyphenols in green teas ranged from 14% to 34%, indicating a large variation, which was not reflected in price. The solubility of total polyphenols from teabags has been proposed as a useful quality index of the filtering paper used for the teabags. This chemical analysis of phenolic compounds in commercial teas may be a potential tool for the quality control of Australian manufactured and imported teas in Australian markets.     

Catechins depletion patterns in relation to theaflavin and thearubigins formation

The determination of the depletion pattern of catechins in black tea processing is important in achieving optimum tea quality. This study investigated catechins (unoxidised di- and trihydroxylated) depletion patterns in relation to theaflavin and thearubigin formation. It was during the process of green leaf fermentation at selected temperature and time combinations. The tea leaves were obtained from three clones (6/8, 303/577 and 311/287) within the Tea Research Foundation of Kenya. The results were showed that unequal depletion rates of di- and trihydroxylated catechins led to a decline in total theaflavin and an increase in thearubigins levels. An equitable decline in both groups of catechins corresponded to a subsequent rise in theaflavins content. The decline in the catechins levels was much faster at higher temperatures resulting in a shorter fermentation time to achieve a peak of the theaflavins content. Clone 311/287 had the highest mean theaflavins content (26.99 μmol/g) and the least mean percent thearubigins (15.02%) level. Theaflavins content correlated positively with liquor brightness determined by a spectrophotometer and tea tasters (r = 0.7221, p < .0001). The thearubigins content was however found to relate negatively with liquor brightness. It was concluded that the experimental conditions tested form a good basis for clonal specific processing conditions that can be utilised in manufacturing quality black tea.