Simultaneous determination of thiabendazole and mebendazole in tablets by high-performance liquid chromatography

Reversed-phase high performance liquid chromatography using an RP 18 column (4 × 125 mm), tetrahydrofuran-acetonitrile-0.5% formic acid (5:25:70, v/v/v) as mobile phase and UV detection at 254 nm enabled the simultaneous determination of thiabendazole (TZ) and mebendazole (MZ) in tablets. The method showed linearity over 4.0 to 40.0 μg TZ/ml and 6.0 to 60.0 μg MZ/ml. The correlation coefficient r was .9999 for both TZ and MZ. The coefficient of variation (CV) was 0.59-0.80% for TZ and 0.49-0.67% for MZ. The average recovery was 100.54-101.17% for TZ and 100.35-101.13% for MZ. The excipients of the tablets did not interfere in the proposed method. The developed method is precise, accurate, and selective for the determination of both benzimidazoles analyzed.     

Reproductive and neurobehavioural effects of thiabendazole administered to mice in the diet

Thiabendazole was given in the diet to provide levels of 0 (control), 0.031, 0.125, and 0.5% from 5 weeks of age of the F₀ generation to 9 weeks of age of the F₁ generation in mice, and selected reproductive and neurobehavioural parameters were measured. The average litter size and weight were significantly reduced in the high-dose group at birth. No adverse effects were observed in the sex ratio at birth. The average body weight of offspring was significantly increased in the low-dose group during the late lactation period, and was significantly reduced in the high-dose group during the lactation period. In the assessment of neurobehavioural parameters, surface righting at postnatal day (PND) 7 was significantly delayed in a dose-related manner in both sexes. Swimming limb movement at PND 14 and olfactory orientation at PND 14 were significantly depressed in the high-dose group in both sexes. In movement activity at 3 weeks of age in the F₁ generation, vertical time and number of defaecations were significantly decreased in the high-dose group in female offspring. Several adverse effects on reproductive and neurobehavioural parameters were produced at the highest dose level of thiabendazole used in the present study (equivalent to 700-1800mg/kg bw/day). Slight, dose-related delays were also seen in surface-righting ability at the two lower dose levels. The lowest dose level (equivalent to 50-180mg/kg bw/day) is 500     

固相萃取-气相色谱质谱联用法同时测定食品接触产品中邻苯基苯酚、噻苯咪唑、联苯及抑霉唑的迁移量

建立了气相色谱质谱联用技术同时测定邻苯基苯酚(OPP)、噻苯咪唑(TBZ)、联苯(DP)及抑霉唑(IMZ)迁移量的方法。样品分别采用水、4%乙酸、20%乙醇、50%乙醇作为食品模拟物,在特定条件下浸泡提取。浸泡液运用固相萃取技术净化提纯后过0.45μm微孔薄膜,在微极性柱上进行分离,质谱检测,外标法定量分析。优化条件下,OPP、TBZ、DP、IMZ在2-800μg/L浓度范围内与其峰面积线性良好,定量限分别为3、5、2和5μg/L(S/N=10)。在3个浓度水平进行添加实验,OPP、TBZ、DP、IMZ的平均回收率在70.4-112%之间,重复性精密度在1.78-6.72%(n=6)之间。结果表明,本方法操作简单快速、重现性好、准确度高。     

Simultaneous Determination of Thiabendazole and Mebendazole in Tablets by High-Performance Liquid Chromatography

Reversed-phase high performance liquid chromatography using an RP 18 column (4 × 125 mm), tetrahydrofuran–acetonitrile–0.5% formic acid (5:25:70, v/v/v) as mobile phase and UV detection at 254 nm enabled the simultaneous determination of thiabendazole (TZ) and mebendazole (MZ) in tablets. The method showed linearity over 4.0 to 40.0 μg TZ/ml and 6.0 to 60.0 μg MZ/ml. The correlation coefficient r was. 9999 for both TZ and MZ. The coefficient of variation (CV) was 0.59–0.80% for TZ and 0.49–0.67% for MZ. The average recovery was 100.54–101.17% for TZ and 100.35–101.13% for MZ. The excipients of the tablets did not interfere in the proposed method. The developed method is precise, accurate, and selective for the determination of both benzimidazoles analyzed.     

噻苯咪唑的研制

介绍噻苯咪唑制备方法以及生产工艺过程中主要的控制数据，该方法工艺简单、投资省、经济效益显著。     

TBZ放大性的研究

介绍了ＴＺＢ的用途,特点及制备方法,并进行了经济评价及讨论,结果证明：ＴＢＺ放大性研究安全可靠,质量接近国外同类产品水平,具有可观的经济效益。     

Simple and fast spectrophotometric determination of low levels of thiabendazole residues in fruit and vegetables after pre-concentration with ionic liquid phase microextraction

There is a great importance of monitoring thiabendazole (TBZ) residues in fruits and vegetables to ensure food safety. Therefore, a new ionic liquid (IL) phase microextraction method using IL, 1-butyl-3-methylimidazoliumhexafluorophosphate [C₄mim][PF₆], as extracting solvent is proposed for simple and fast determination of low levels of TBZ in fruits and vegetables by spectrophotometry. The method is based on selective complex formation of TBZ with Cu(II) ions in presence of PF₆^– as counter ion at pH 5.5, and then microextraction of the complex into the fine micro-drops of IL phase. After optimisation of variables affecting microextraction efficiency, the analytical parameters of the method were determined by calibration curves. The method exhibits a linear relationship (0.3–280 μg L^?1), low detection limit (0.1 μg L^?1), good intra- and inter-day precision (2.4–4.5% as RSD_r%, 2.1–5.6% as RSD_R%), good recovery (≥95.1–98.2%) and high sensitivity enhancement factor (150) by solvent-based calibration curve. It allows a detection limit of 0.24 μg L^?1 and a range of 0.8–250 μg L^?1 by the matrix-matched calibration curve. After validation, the method was successfully applied to the determination of TBZ residues with method quantification limits in fruit and vegetables of 2.0 and 2.5 µg kg^?1 with and without adding polyvinylpyrrolidone (PVP-15) solution. Recoveries range from 85.5% to 98.2% after spiking (10, 50 and 100 µg kg^?1, n: 3).