全文获取类型
收费全文 | 260篇 |
免费 | 20篇 |
国内免费 | 17篇 |
专业分类
综合类 | 20篇 |
化学工业 | 83篇 |
金属工艺 | 3篇 |
机械仪表 | 14篇 |
建筑科学 | 5篇 |
能源动力 | 1篇 |
轻工业 | 111篇 |
无线电 | 13篇 |
一般工业技术 | 29篇 |
冶金工业 | 9篇 |
自动化技术 | 9篇 |
出版年
2024年 | 2篇 |
2023年 | 4篇 |
2022年 | 13篇 |
2021年 | 10篇 |
2020年 | 16篇 |
2019年 | 8篇 |
2018年 | 8篇 |
2017年 | 11篇 |
2016年 | 9篇 |
2015年 | 10篇 |
2014年 | 15篇 |
2013年 | 18篇 |
2012年 | 19篇 |
2011年 | 27篇 |
2010年 | 16篇 |
2009年 | 15篇 |
2008年 | 16篇 |
2007年 | 20篇 |
2006年 | 19篇 |
2005年 | 11篇 |
2004年 | 5篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2001年 | 2篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1988年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有297条查询结果,搜索用时 0 毫秒
1.
2.
纳米金-壳聚糖-血红蛋白/普鲁士蓝/金电极检测过氧化氢 总被引:1,自引:0,他引:1
在普鲁士蓝修饰的金电极上,通过纳米金/壳聚糖复合膜固定血红蛋白,研制了安培型过氧化氢生物传感器,线性范围和检测限分别为1~5 000 umol/L和0.27umol/L. 相似文献
3.
本文通过对一氧化碳中毒的机理再研究及对各大医院临床实践的调研,提出了一种全功能报警的报警方式。在预防一氧化碳中毒,提高仪器的安全可靠工作方面进行了有益的探索。 相似文献
4.
吴益华 《上海第二工业大学学报》2010,27(1):1-6
通过对层状纤铁矿型钛酸锂钾进行剥离,可以制备得到Ti0.865O2纳米片溶胶。将Ti0.865O2纳米片作为载体固定血红蛋白(Hb),制备得到Hb/Ti0.865O2/纳米片修饰的热解石墨(PG)电极。在Ti0.865O2纳米片层间,Hb能实现与电极的直接电子转移。在pH值为5.6的磷酸盐缓冲溶液中,该修饰电极的循环伏安曲线上显示出一对可逆的氧化还原峰,式电位为-230mV(vs.Ag/AgCl)。该修饰电极对H2O2有良好的电催化响应,线性响应范围为50μM到2.2mM,灵敏度为10μA.mM^-1.cm^-2。 相似文献
5.
Yu‐Jung Lin Chun‐Chieh Chen Dang Nguyen Huei‐Rou Su Kun‐Ju Lin Hsin‐Lung Chen Yu‐Jung Hu Po‐Liang Lai Hsing‐Wen Sung 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(23)
Nitric oxide (NO) is a potent tumor‐cell radiosensitizer but it can be readily scavenged by hemoglobin (Hb) in vivo. A biomimetic incubator that can generate and deliver NO in a scavenger (Hb)‐free environment to enhance its radiosensitizing effect to maximize its efficacy in radiotherapy is proposed. This NO incubator comprises a poly(lactic‐co‐glycolic acid) (PLGA) hollow microsphere (HM) that contains an NO donor (NONOate) and a surfactant molecule (sodium caprate, SC) in its aqueous core. In acidic tumorous environments, the PLGA shell of the HM allows the penetration of protons from the outside, activating the hydrolytic cleavage of NONOate, spontaneously generating NO bubbles, which are immediately trapped/stabilized by SC. The SC‐stabilized NO bubbles in the HM are then squeezed through the spaces of its PLGA matrices by the elevated internal pressure. Upon leaving the HM, the entrapped NO molecules may passively diffuse through their SC‐stabilized/protected layer gradually to the tumor site, having a long‐lasting radiosensitizing effect and inhibiting tumor growth. The entire process of NO generation and delivery is conducted in a scavenger (Hb)‐free environment, mimicking the development of young ovoviviparous fish inside their mothers' bodies in the absence of predators before birth. 相似文献
6.
Byung Hoon Jo Jaoon Y.H. Kim Jeong Hyun Seo Hyung Joon Cha 《International Journal of Hydrogen Energy》2014
H2 production under aerobic conditions has been proposed as an alternative method to overcome the fundamentally low yield of H2 production by fermentative bacteria by maximizing the number of electrons that are available for H2. Here, we engineered Vitreoscilla hemoglobin (VHb) in Escherichia coli to study the effects of this versatile oxygen (O2)-binding protein on oxic H2 production in a closed batch system that was supplemented with glucose. The H2 yields that were obtained with the VHb-expressing E. coli were greatly enhanced in comparison to the negative control cells in culture that started with high O2 tensions. The formate hydrogen lyase (FHL) activity of oxically cultured, VHb-expressing cells was also much higher than that of the negative control cells. Through inhibitor studies and time-course experiments, VHb was shown to contribute to the improved H2 yield primarily by increasing the efficiency of cellular metabolism during the aerobic phase before the onset of H2 production and not by working as an O2-scavenger during H2 production. This new approach allowed more substrate to remain to be further utilized for the production of more H2 from limited resources. We expect that VHb can be successfully engineered in potential aerobic H2-producing microbial systems to enhance the overall H2 production yield. In addition, the remarkably high FHL activity of oxically grown, VHb-expressing cells may make this engineered strain an attractive whole-cell biocatalyst for converting formate to H2. 相似文献
7.
Dendrimers are novel three dimensional, hyperbranched globular nanopolymeric architectures. Attractive features like nanoscopic size, narrow polydispersity index, excellent control over molecular structure, availability of multiple functional groups at the periphery and cavities in the interior distinguish them amongst the available polymers. Applications of dendrimers in a large variety of fields have been explored. Drug delivery scientists are especially enthusiastic about possible utility of dendrimers as drug delivery tool. Terminal functionalities provide a platform for conjugation of the drug and targeting moieties. In addition, these peripheral functional groups can be employed to tailor-make the properties of dendrimers, enhancing their versatility. The present review highlights the contribution of dendrimers in the field of nanotechnology with intent to aid the researchers in exploring dendrimers in the field of drug delivery. 相似文献
8.
采用胰酶和Protamex(的复合酶系结合响应面法研究了温度、pH值、水料比、加酶量和酶解时间对从猪血血红蛋白中提取可溶蛋白、肽及氨基酸的影响规律。数学模型优化工艺条件如下:温度为49.4℃;pH值为7.7;水料比为1.4∶1;加酶量为2.0 g/kg;酶解时间为18.6h。模型氮回收率的预测值为97.98%,实测值为96.94%。>15000 Da、15000—5000 Da、5000—1000 Da、<1000 Da的4个分子量段的酶解产物含量分别为21.1%、3.9%、20.9%、54.1%。酶解产物没有苦味,与根据Q准则计算的疏水性值结果吻合,可作为食品原料在食品生产中广泛应用。 相似文献
9.
血液中血红蛋白含量的测定 总被引:3,自引:0,他引:3
利用电合成方法在玻碳电极表面制备聚硫堇薄膜,并用电化学及石英电子微天平方法研究其对血红蛋白的吸附作用.吸附态血红蛋白具有直接的电化学行为,且其氧化-还原电流与血红蛋白(Hb)的含量成正比关系,可用于血液中血红蛋白含量的测定. 相似文献
10.
目的 研究猪血红蛋白的酶解工艺.方法 用组合酶Ⅰ(蛋白酶F与蛋白酶P)和组合酶Ⅱ(蛋白酶F与蛋白酶N)同步酶解猪血红蛋白.结果 确定最佳工艺参数,组合酶Ⅰ的最佳酶解条件为:酶添加量0.2%,温度50℃,料水比1∶1.25,pH 7.5,水解时间20 h;组合酶Ⅱ的最佳酶解条件为:酶添加量0.24%,温度50℃,料水比1:1.75,pH 8.0,酶解时间20h.此工艺条件下,组合酶Ⅰ和组合酶Ⅱ三氯乙酸可溶性氮含量(TCA-SN指数)分别为71.72%和87.82%,两种组合酶的蛋白质回收率均达90%.结论 组合酶Ⅱ较适于工业生产. 相似文献