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P Sarkar D Ghosh D Bhattacharya R Kataky SJ Setford SF White APF Turner 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2005,80(12):1389-1394
Allergen levels in indoor environments, leading to many diseases, eg asthma, rhinitis and conjunctivitis, affect a large and increasing fraction of the population. A quite effective and inexpensive method of a rough but very rapid overall assessment of total allergen level in the environment has been developed. The method involved estimation of protein in allergen extracts by screen‐printed electrodes using two different techniques. The biosensor comprised a rhodinised carbon working electrode, a silver/silver chloride reference electrode and a carbon counter electrode. In the first method the enzyme protease reacted with allergen protein to release amino acid, which produced hydrogen peroxide in the presence of amino acid oxidase. This was detected amperometrically. The second method used potassium bromide as electrolyte and the electrode was subjected to dual potential. Bromine, released due to electrolysis at higher potential, was consumed by the allergen protein at lower potential. In the first method, a unique technique was used to microencapsulate the enzyme protease and immobilise it on the surface of the electrode by in‐situ polymerisation to avoid contact with the amino acid oxidase. A total of seven allergens were tested and the results gave a good correlation with the standard protein measurement method. Environmental specimens from indoors, schools and workplaces can be evaluated for the aeroallergens produced by dust mites, animal hairs, cockroach debris, pollens, etc as a means of determining the exposure risk. Copyright © 2005 Society of Chemical Industry 相似文献
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Yinjun Wang Xuguang Wang Shilong Yah 《北京科技大学学报(英文版)》2006,13(2):102-107
The desensitization degree of emulsion explosives (EE) was calculated with the peak pressure of explosion shock waves tested in water. To an explosive, the less the desensitization degree, the better the compression resistance, so the compression resistance of an explosive can be compared and analyzed quantificationally with the desensitization degree. The influence of an emulsifier on the pressure desensitization of EE was studied, including the content and category of emulsifiers. Three kinds of emulsifiers (Span-80, compound emulsifier, and T-152) were used in the tests. The experimental results show that both the content and category of emulsifiers make a great effect on the pressure desensitization of EE. The desensitization degree of EE reduces with the emulsifier content being increased, but there is an optimal content of an emulsifier for the compression resistance of EE. While the content of Span-80 reaches 4wt%, the desensitization degree of EE becomes a minimal value, and augments somewhat if the emulsifier content is increased more. That is to say, the compression resistance of EE becomes the highest while the content of Span-80 is 4wt%, and the compression resistance will decline if the content of Span-80 is increased more. The compression resistance of the explosive emulsified by compound emulsifier is the highest among all the explosives, when the content of the whole components and manufacturing engineering are kept invariable. 相似文献
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Silver Steven M.; Rogers Susan; Knipe James; Colelli Gina 《Canadian Metallurgical Quarterly》2005,12(1):29
This article presents the results of a time-limited psychological relief effort using eye movement desensitization and reprocessing (EMDR) following the attacks on the World Trade Center on September 11, 2001. Clients made highly significant positive gains on a range of outcome variables, including validated psychometrics and self-report scales. Analyses of the data suggest 2 broad conclusions: EMDR is a useful treatment intervention both in the immediate aftermath of disaster as well as later; the longer treatment is delayed, the greater the level of disturbance experienced by clients. Also discussed are problems in conducting research during mass disaster response situations. A demonstration of an analog to a wait-list control group is provided. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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《Food Control》2015
Tree nut allergies are considered an important health issue in developed countries. To comply with the regulations on food labeling, reliable allergen detection methods are required. In this work we isolated almond-specific recombinant antibody fragments (scFv) from a commercial phage display library bypassing the use of live animals, hence being consistent with the latest policies on animal welfare. To this end an iterative selection procedure employing the Tomlinson I phage display library and a crude almond protein extract was carried out. Two different almond-specific scFv (named PD1F6 and PD2C9) were isolated after two rounds of biopanning, and an indirect phage ELISA was implemented to detect the presence of almond protein in foodstuffs. The isolated scFvs demonstrated to be highly specific and allowed detection of 40 ng mL−1 and 100 ng mL−1 of raw and roasted almond protein, respectively. The practical detection limit of the assay in almond spiked food products was 0.1 mg g−1 (110–120 ppm). The developed indirect phage ELISA was validated by analysis of 92 commercial food products, showing good correlation with the results obtained by a previously developed real-time PCR method for the detection of almond in foodstuffs. The selected phage clones can be affinity maturated to improve their sensitivity and genetically engineered to be employed in different assay formats. 相似文献
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针对互联网形式下大数据剧增,敏感、隐私数据容易丢失的问题,提出一种大数据脱敏技术方案,构建出包含资源层、数据层、服务层以及应用层的B/S的管理架构,能够在用户进行信息交互时,保护用户的敏感隐私信息,并对大数据脱敏方法进行了介绍,本文设计的方案能够有效地不同数据库中的敏感数据识别出来,并针对营销数据库、人工智能数据库、门... 相似文献
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