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1.
Dr. Alejandra A. Carriles Alberto Mills Dr. María-José Muñoz-Alonso Dr. Dolores Gutiérrez Dr. Juan M. Domínguez Prof. Dr. Juan A. Hermoso Prof. Dr. Federico Gago 《Chembiochem : a European journal of chemical biology》2021,22(2):374-391
Spontaneous mutations in the EEF1A2 gene cause epilepsy and severe neurological disabilities in children. The crystal structure of eEF1A2 protein purified from rabbit skeletal muscle reveals a post-translationally modified dimer that provides information about the sites of interaction with numerous binding partners, including itself, and maps these mutations onto the dimer and tetramer interfaces. The spatial locations of the side chain carboxylates of Glu301 and Glu374, to which phosphatidylethanolamine is uniquely attached via an amide bond, define the anchoring points of eEF1A2 to cellular membranes and interorganellar membrane contact sites. Additional bioinformatic and molecular modeling results provide novel structural insight into the demonstrated binding of eEF1A2 to SH3 domains, the common MAPK docking groove, filamentous actin, and phosphatidylinositol-4 kinase IIIβ. In this new light, the role of eEF1A2 as an ancient, multifaceted, and articulated G protein at the crossroads of autophagy, oncogenesis and viral replication appears very distant from the “canonical” one of delivering aminoacyl-tRNAs to the ribosome that has dominated the scene and much of the thinking for many decades. 相似文献
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从徐淮地区井筒破裂的特点出发,分析引起井壁破裂的根本原因,据此提出井筒的破裂机理,为选择防治井筒破裂的方法提供依据 相似文献
5.
牙轮钻头的岩屑破碎机理及可钻性的分形法 总被引:3,自引:0,他引:3
为满足优质高速钻井的要求,需要对岩石可钻性做出快捷、精确的判断。利用分形几何理论,研究了钻井过程中牙轮钻头的破岩机理以及岩屑的等破碎概率问题;依据Bond裂纹学说,提出了一种以井底返出的岩屑为对象的岩石可钻性测试方法,并建立了岩石可钻性极值和岩屑分形维数的关系模型。室内实验表明:微钻头岩石可钻性级值与破碎岩屑分形维数存在明显的相关性,由此制定了一个基于岩屑粒度分布分形维数表示的岩石可钻性预测指标。经现场检验,分形法确定岩石可钻性级值具有较高的精度。这表明钻井过程中上返岩屑的分形维数能反映岩石可钻性程度,可以用分形维数衡量岩石可钻性大小。 相似文献
6.
主要分析了电解多功能天车打壳机构中打击头、夹套、拉杆非正常失效的原因,并提出了解决的方案. 相似文献
7.
Water extracts of giant foxtail grass inhibited germination and radicle elongation of loblolly pine seed in a laboratory test. Most of the toxic effects came from extracts of dried foxtail tops, with lesser amounts from fresh tops and roots. Soil texture affected the phytotoxicity of the extracts. Eight chemical compounds known to be phytotoxins inSetaria were identified in extracts of foxtail leaves but could not be found in soil samples under the leaves. 相似文献
8.
The effect of plasma elongation on the second‐stable spherical tokamak (ST) was numerically studied using the experimentally measured pressure and current profiles of ultrahigh‐beta STs. The maximum beta of ST over 50% was obtained in the TS‐3 ST/CT experiment by applying an external toroidal field to an FRC. It was found that the marginal beta for the ballooning instability increased with the plasma elongation κ of ST. The elongated STs with κ > 2 have the magnetic shear (S)–pressure gradient (α) profiles located in the second‐stable regime for the ballooning mode and the stability margin increased with κ. The close relation between the absolute minimum‐B profile and the second stability was documented. The effect of elongation on maximum beta was observed to saturate when κ exceed 3, indicating that the optimized elongation for high‐beta STs is located around 2 < κ < 3. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(4): 1–6, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20132 相似文献
9.
Nelly RahmanApriyanti Isanasari Ririn AnggraeniSuharto Honggokusumo Masatoshi Iguchi Toru MasukoKohji Tashiro 《Polymer》2003,44(1):283-288
To investigate the mechanism of high elongation of natural rubber attained by the ‘racking method’, a strip of smoked-sheet was elongated up to 150 times in length, by stretching rapidly at room temperature and forcing to shrink at 75-80 °C repeatedly. On X-ray diffraction, a typical fibre pattern with an amorphous ring appeared already at ×10 (stretch ratio=10). The degree of crystallinity increased to the level of 17-18% at ×20 and turned to decrease after ×60, but the degree of crystallite orientation reached at a high level already at ×10 and did not change significantly and the half-height width of reflection profiles stayed almost at a constant level, while the Young's modulus increased up to ca. 300 MPa at ×100 and then fell rather discontinuously. It was assumed that some fractions of chain segments between entanglements were gradually broken in each step of stretching operation, rather than disentangled and slipped. 相似文献
10.
T. Brard F. H. Cornet 《International Journal of Rock Mechanics and Mining Sciences》2003,40(7-8):1121-1140
When they occur, borehole breakouts are considered strong markers of principal stress directions at depth. An innovative processing method for automatically identifying breakouts from ultrasonic borehole wall images has been developed. It has been applied to data sets from two deep, sub-vertical wells (GPK1 and GPK2) at the Soultz geothermal site in eastern France. In well GPK1, below 3 km depth, compression breakouts, with a 95°±7° azimuth, increasingly occur with depth. They result from time dependent compression failure at sub-critical stress levels and are indicators of the minimum horizontal principal stress orientation. However, in the uppermost logged section of well GPK2 (1.6–2.9 km depth), continuous borehole elongations share roughly the same azimuth with so called drilling-induced fractures (164°±18° and 175°±17° azimuth, respectively). Both features concomitantly vanish with depth, together with the amplitude of the thermal perturbation induced by drilling. It is proposed that these latter borehole elongations result from a pervasive, cooling-induced, tensile micro-cracking process prior to macroscopic failure localization. They are termed thermal elongations and are indicators of the maximum horizontal principal stress orientation. Had a simple logging caliper tool been used for this work, these thermal elongations might have been confused with classical compression breakouts. A simple criterion for differentiating compression breakouts from thermal elongation is proposed. 相似文献