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1.
Tumor cell aggregation is critical for cell survival following the loss of extracellular matrix attachment and dissemination. However, the underlying mechanotransduction of clustering solitary tumor cells is poorly understood, especially in non-small cell lung cancers (NSCLC). Here, we examined whether cell surface protrusions played an important role in facilitating the physical contact between floating cells detached from a substrate. We employed poly-2-hydroxyethyl methacrylate-based 3D culture methods to mimic in vivo tumor cell cluster formation. The suprastructural analysis of human NSCLC A549 cell spheroids showed that finger-like protrusions clung together via the actin cytoskeleton. Time-lapse holotomography demonstrated that the finger-like protrusions of free-floating cells in 3D culture displayed exploratory coalescence. Global gene expression analysis demonstrated that the genes in the organic hydroxyl transport were particularly enriched in the A549 cell spheroids. Particularly, the knockdown of the water channel aquaporin 3 gene (AQP3) impaired multicellular aggregate formation in 3D culture through the rearrangement of the actomyosin cytoskeleton. Moreover, the cells with reduced levels of AQP3 decreased their transmigration. Overall, these data indicate that cell detachment-upregulated AQP3 contributes to cell surface protrusions through actomyosin cytoskeleton remodeling, causing the aggressive aggregation of free-floating cells dependent on the property of the substratum and collective metastasis. 相似文献
2.
Ming-Puu Chen Asta Y. Z. Lord Yu-Yao Cheng Ku-Chou Tai Wen-Harn Pan 《Journal of Computer Assisted Learning》2020,36(3):383-396
Reflective judgement is crucial for medical-related practitioners in dealing with controversial issues. However, the conformity phenomenon is likely to occur and interfere with reflective judgement learning during interactive activities. Effective strategies are required to moderate the conformity behaviour tendency (CBT) and improve reflective judgement performance (RJP). This study demonstrates two significant results: (a) Compared with the guided self-reflection learning strategy, the online collective reflection (OCR) learning strategy effectively weakened the learners' general CBT while dealing with professional controversial issues; and (b) a significantly negative correlation between the RJP achieved and the change of CBT in online environment was detected in the OCR group. The implications and potential applications in higher education were discussed. Further studies are needed to confirm the long-term effects and the extending application to other professional studies. 相似文献
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Comments on an article by Eidelson and Eidelson (see record 2003-03645-004) which made an important contribution, at a critical juncture, to the discussion of international conflicts by identifying core dimensions that allow for the systematic examination of the problem. Their article also posed a danger that mirrors a disturbing trend in the public discourse about conflicts and injustice. In response to the recent escalation of international violence and years of mixed results of engagement in debates centered around grievances of injustice (such as the Palestinian-Israeli conflict), many politicians, pundits, and scholars have advocated addressing conflicts by separating them from the underlying grievances and the ongoing injustice. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
6.
Myers Nicholas D.; Payment Craig A.; Feltz Deborah L. 《Canadian Metallurgical Quarterly》2004,8(3):182
This study examined reciprocal relationships between collective efficacy and team performance over a season of competition in women's intercollegiate ice hockey within weekends where the opponent was constant for 2 games. Collective efficacy beliefs within 12 teams were assessed prior to both games for at least 7 weekends. Team performance indexes produced an overall measure of performance for each game. The average influence of Saturday collective efficacy on Saturday performance was moderate and positive after controlling for Friday performance. The average influence of Friday performance on Saturday collective efficacy was small and positive after removing the influence of Friday collective efficacy from Friday performance. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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本文介绍了广播发射台利用计算机技术、网络技术、遥控遥测技术、现代测试手段,对设备进行自动化控制与管理;实现设备技术指标自动测试,发射台无人值班、有人留守,建立机器的计算机档案制度,确保安全优质播出的方法和经验。 相似文献
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李文军 《沙洲职业工学院学报》2006,(4)
忘记历史就意味着背叛,张抗抗在《集体记忆》中描写史志工作者在搜集史料时遇到了这种背叛现象,知情人有意无意地患上了“集体忘却”症。通过梳理此症的主要表现,即强占记忆与摧毁记忆,指出其社会文化政治因素在于建国以来的主流思想。 相似文献
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政治共同民主为经济共同富裕提供制度基础和政治保障。其中,民主政治是实现共同富裕的前提,建立有效、灵活和合理的政府是关键,健全法制是实现共同富裕的保障。文化共同繁荣为共同富裕提供精神动力和智力支持。 相似文献
10.
鱼群中的个体如何通过信息传递从而达到一致的群体运动状态,至今还没有刻画这一现象的统一数学模型.本文阐述了一种利用视频数据和传递熵构建鱼群中个体间信息传递网络的方法.首先用实验获取斑马鱼集群的视频数据,并采用计算机视觉跟踪的方法获取鱼群中每个个体的位置和运动速度,然后利用传递熵计算个体两两之间的信息传递关系,在此基础上构建了鱼群信息传递网络.通过网络分析,揭示了鱼群中个体间的信息交互个数与信息传播速度之间的关系,进一步发现了鱼群信息传递网络中的频繁子结构.本文提供了一种利用探测时间序列间因果关系建立鱼群信息传递网络的方法,为鱼群信息传递研究提供了一种新的思路. 相似文献