排序方式: 共有63条查询结果,搜索用时 15 毫秒
1.
2.
酶解法提取虎杖中白藜芦醇、白藜芦醇苷、大黄素 总被引:1,自引:0,他引:1
用纤维素酶对虎杖进行了酶解,提高了虎杖中白藜芦醇的提取率,同时提取出白藜芦醇苷和大黄素。通过单因素实验考察了酶解温度、pH、酶解时间、酶用量对目标物提取率的影响,得出较佳的酶解工艺条件为:酶用量2mg/g虎杖,pH=3.0,在40℃酶解1h。酶解后用m(虎杖):m[φ(乙醇)=60%]=1:20,50℃提取2h。w(白藜芦醇)=1.50%,w(白藜芦醇苷)=0.38%,w(大黄素)=0.78%。酶解法较直接提取法更有利于虎杖中有效成分白藜芦醇、大黄素的提取。 相似文献
3.
白藜芦醇是一种生物性很强的天然多酚类物质,又称为芪三酚,是动脉粥样硬化、心脑血管疾病、肿瘤的化学预防剂,主要来源于花生、葡萄(红葡萄酒)、虎杖、桑椹等植物。本文综述了近十几年来虎杖白藜芦醇提取工艺,并分析比较各种方法的优缺点和发展趋势,以期为虎杖白藜芦醇提取工艺的深入研究提供参考。 相似文献
4.
5.
白藜芦醇提取工艺的研究 总被引:3,自引:0,他引:3
本研究探讨了虎杖中白藜芦醇的提取工艺和稳定性。在虎杖中,白藜芦醇对光、干热、湿热和氧化剂均敏感;通过实验得出回流、超声、固定床连续提取的提取率分别0.201%、0.423%、0.513%;同时利用正交设计得出固定床连续提取的最佳工艺为溶剂为30%乙醇,固定床连续提取速度为8ml/min·kg,浸泡时间为12h,溶剂用量为8倍量。 相似文献
6.
7.
虎杖中抗氧化成分的提取分离及其活性研究 总被引:11,自引:2,他引:11
提取了虎杖中具有抗氧化活性的总蒽醌(TA),并从总蒽醌中依次分离出强酸成分(SAP)、中酸成分(MAP)和弱酸成分(WAP)。然后采用二苯代苦味酰自由基(DPPH)法对不同质量浓度的TA、SAP、MAP和WAP进行了抗氧化活性测定。TA、SAP、MAP和WAP质量浓度分别为0.2 g/L、0.5 g/L、0.8 g/L、1.2 g/L时,对自由基的最大清除率依次分别为:29.5%、67.1%、85.7%、87.5%;34.0%、74.2%、86.5%、95.4%;9.8%、24.0%、35.2%、47.3%;6.5%、11.1%、19.6%、19.9%。TA、SAP、MAP和WAP对自由基均有程度不同的清除作用,其中SAP的效果最佳,当其质量浓度为1.2 g/L时,最大清除率可高达95.4%。 相似文献
8.
Optimization of extraction and determination of emodin from Polygonum cuspidatum Sieb. et Zucc. products by HPLC-DAD 总被引:1,自引:0,他引:1
A uniform experimental design procedure was used to investigate the effects of some operating parameters on the extraction of emodin from Polygonum cuspidatum Sieb. et Zucc. products. Variables tested were volume ratio of material to solvent, size of material, extraction time and temperature and composition of extraction solvent (mixtures of acetone-water). Each variable was tested at seven levels; 7 experiments were performed in random order. Analyses of the extracts were performed by high-performance liquid chromatography with diode array detection(HPLC-DAD). Analytical responses were processed by using a forward regression analysis, in order to find polynomial function describing the relationship between variables and responses. For all the analytes the experimental conditions for providing the highest extraction yield inside the experimental domain considered were found. Finally, a simple, rapid and accurate analytical method was developed for the determination of emodin by high performance liquid chromatography. The separation is achieved within 25 rain on an ODS column using methanol and water as gradient mobiles. Emodin can be quantified by using external standard method detecting at 436 nm. Good linearity is obtained with correlation coefficient exceeding 0.9986 and the detection limit and the quantification limit are 1.53 and 3.23 mg/L respectively. This method shows good reproducibility for the quantification of the emodin with intra-day and inter-day relative standard deviation less than 2.3% and 5.6% respectively. Under optimized extraction conditions, the recovery of the standard is 96.5%. The validated method is successfully applied to quantify the emodin in seven Polygonum cuspidatum sieb. Et zucc. products, which provided an idea for the pre-treatment of determination of active compounds in traditional Chinese medicines. 相似文献
9.
大孔吸附树脂对虎杖中白藜芦醇吸附性能的研究 总被引:7,自引:0,他引:7
本文研究了多种大孔吸附树脂对白藜芦醇的吸附与脱附性能,从中选出H103树脂具有较大吸附量和解吸率。动态吸附实验研究了提取液浓度、pH、流速对H103树脂吸附量的影响,适合的上柱浓度为0.7157mg/ml,上柱液的pH为4.10,上柱液流速为2BV/h。4倍树脂床体积的80%乙醇以1BV/h的流速进行洗脱即可基本将白藜芦醇完全从H103树脂上解吸下来。 相似文献
10.
微生物酶法从虎杖中提取白藜芦醇的研究 总被引:1,自引:0,他引:1
用微生物发酵法酶解虎杖,可以提高从虎杖中获取白藜芦醇的得率.通过对多种含糖甙键水解酶菌株的筛选,获得四株可以直接作用于虎杖的优良菌株.其中用 Aspergillus niger 1分解虎杖,提取白藜芦醇,与传统醇提工艺和自酶解法相比,提取率明显提高. 相似文献