Recovery of residual nutrients from agri-food byproducts using a combination of solid-state fermentation and insect rearing

The present research develops an innovative nutrient recovery strategy for capturing of un-utilised nutrients from agri-food byproducts using a combination of solid state fermentation (SSF) and insect rearing. SSF of borage and flaxseed meals were performed using GRAS organisms to release indigenous nutrients and to produce additional nutrients. Proximate analysis of the SSF-meals showed increases in both lipid and protein contents. Black soldier fly larvae (BSFL) were then grown on these SSF-meals for 12 day. The meals fermented singularly with Lactobacillus plantarum or Aspergillus niger displayed up to 30% enhancement in BSFL biomass, and dual fermentation with L. plantarum and Aspergillus oryzae resulted in an additional 10% enhancement. These examinations showed SFL use over-90% of proteins and lipids in SSF-meals. The results indicate that fermentation of these low-value meals can boost the efficacy of larval growth and the recovery of nutrients from agricultural byproducts as larval biomass.     

A self-report measure of attitudes toward the eating of insects: construction and validation of the Entomophagy Attitude Questionnaire

Research has made steps towards the understanding of the main drivers and barriers related to Western consumers’ acceptance of food with ingredients derived from insects. Nevertheless, research has been hampered by the absence of a comprehensive, specific, and validated instrument for measuring consumer attitudes towards entomophagy. The current study presents a new self-report instrument for addressing this issue. The instrument was built upon three conceptual cores, which are reflected in the three factors emerged and tested through factor analyses performed on a representative sample of the Danish population (Study 1: N = 975), and a convenience Italian sample (Study 2: N = 543). The first factor represents the negative evaluation of direct entomophagy and is closely connected to disgust. The second factor represents the positive evaluation of direct entomophagy and is closely linked to individuals’ interest to try novel experiences and to eat novel foods. The third factor addresses indirect entomophagy, that is, the attitude towards using insects for feeding other animals that are reared for human consumption. Compared to existing measures of disgust and neophobia, the new instrument performs better in predicting intention in relation to both direct and indirect entomophagy.     

Acceptance of insect-based food in Chile: Evidence from a survey using the entomophagy attitude questionnaire (EAQ)

Research interest in the acceptability of insects as food has dramatically grown in recent years. Investigators have studied the antecedents of people willingness to eat whole insects, processed (non-visible) insects, and insect-fed animals as well. Another important topic is the difference between intention to try insect-based foods once, and readiness to adopt these foods in everyday diet. In 2020, the Entomophagy Attitude Questionnaire has been developed as a tool for researchers in this area. It is the only one self-report questionnaire specific to entomophagy, multi-dimensional, and cross-validated in diverse contexts and languages. The current study contributes to further validate this instrument in Spanish, in a novel context such as Chile, and to expand its nomological validity to intention to eat processed insects. In addition, results show that the three components of the EAQ significantly predict participants’ intention to try insect-based foods and, to a lesser extent, intention to add insect-based food in one own’s diet. Limitations of the study, as well as direction for further research are discussed in the paper.     

Effects of cricket powder on selected physical properties and US consumer perceptions of whole-wheat snack crackers

Successful introduction of insect consumption to a reluctant US population requires novel insect-based foods that are responsive to consumers’ expectations of sensory quality. Based on our ‘product appropriateness’ data, snack crackers were formulated with increasing levels of cricket powder (0%, 5%, 10%, 15%, and 20%) in substitution of whole-wheat flour. Colour, texture, flavour and overall perceptions of snack crackers were evaluated by 150 US consumers in terms of preference (2-alternative choice), degree of liking (9-point scale) and acceptability (yes/no). Flavour liking was most highly correlated with overall liking (r = 0.93). Instrumental analyses indicated that increasing cricket powder resulted in darker and harder crackers, negatively impacting colour and texture preference at 5% and 15% cricket powder addition, respectively. Snack crackers remained acceptable at 15% wheat flour substitution (80% acceptability; mean overall liking = 5.5). However, to promote future consumption, an upper limit of 7.9% cricket powder addition is recommended until sensory quality can be improved.     

Ugly but tasty: A systematic review of possible human and animal health risks related to entomophagy

Background: According to many recent studies, the use of insects as food seems to be convenient, sustainable, economical and healthy. The objective of this study is to analyze the possible effects of insect consumption on human and animal health. Methods: A systematic review of the literature was performed using the PubMed, Scopus and CAB databases. Results: Of the 6026 items initially retrieved, 70 were eligible for inclusion; 40 studies analyzed the use of insects in human foods or drugs, while 30 analyzed the use of insects in animal feed. In humans, the most commonly analyzed risks are nutrient malabsorption, growth alteration, chemical and microbiological contamination and allergy risk. Studies of animals focus on growth alteration, nutrient malabsorption and hematic and qualitative meat alteration. Conclusion: In recent years, researchers have shifted their focus from the possible use of edible insects in animal feed to their use as possible nutrient sources for humans. The results suggest that, if properly treated and preserved, products derived from insects are safe and efficient sources of nutrients for animals. Further studies are needed to evaluate the possible effects of prolonged insect consumption on human health.     

Antioxidant activity of predigested protein obtained from a range of farmed edible insects

This study investigated the antioxidant activities of peptides obtained by in vitro gastrointestinal digestion of edible insects. The antioxidant potential of edible insects' hydrolysates was determined as the free radical‐scavenging activity, ion chelating activity and reducing power. The highest antiradical activity against DPPH^˙, whose IC₅₀ value is the lowest, was noted for Amphiacusta annulipes (19.1 μg/mL) and that against ABTS^˙+ was the highest for Zophobas morio (4.6 μg/mL). The peptides obtained from A. annulipes also showed the highest Fe²⁺ chelation ability (58.82%) and reducing power (0.652). The highest ability to chelate Cu²⁺ was noted for Locusta migratoria (86.05%). The locust was characterised by the highest concentration of peptides before digestion and after digestion (3.13 and 5.88 mg/mL, respectively), and the DH was 36.29%.     

Food of the Future? Consumer Responses to the Idea of 3D-Printed Meat and Insect-Based Foods

3D printing technologies are beginning to be employed to fabricate new food products. One of the more unusual and potentially controversial adoptions of this novel food technology involves the use of laboratory-cultured meat or insect-based ingredients to support ethical consumption, food security, and environmental sustainability initiatives. In this article, we discuss findings from our study involving an online discussion group with Australian participants concerning their attitudes to a novel technology that combined new food processing and presentation devices (the 3D printer) with a range of ingredients. The participants recognized the potential benefits of insect- and cultured-meat-based printed food for society. However, their own priorities for food consumption choices centered more on the qualities of health, taste, and naturalness. We found that overall, few participants articulated interest in or support for consuming or serving 3D-printed food products made from cultured meat or insects. Most expressed their disquiet about the degree of processing cultured meat undergoes. It was considered to be “unnatural,” and therefore not fresh, potentially harmful, lacking taste or not nutritious. Most of the participants also expressed antipathy towards the idea of eating printed products containing insects. While such products were considered more “natural” and nutritious than cultured meat, the idea of eating insects was predominantly greeted with disgust. As these findings suggest, plans to use 3D printing technologies to render ingredients such as cultured meat and insects more acceptable and appealing to consumers in countries like Australia have major cultural barriers to overcome.     

Edible insects – defining knowledge gaps in biological and ethical considerations of entomophagy

ABSTRACT

While seeking novel food sources to feed the increasing population of the globe, several alternatives have been discussed, including algae, fungi or in vitro meat. The increasingly propagated usage of farmed insects for human nutrition raises issues regarding food safety, consumer information and animal protection. In line with law, insects like any other animals must not be reared or manipulated in a way that inflicts unnecessary pain, distress or harm on them. Currently, there is a great need for research in the area of insect welfare, especially regarding species-specific needs, health, farming systems and humane methods of killing. Recent results from neurophysiological, neuroanatomical and behavioral sciences prompt caution when denying consciousness and therefore the likelihood of presence of pain and suffering or something closely related to it to insects. From an animal protection point of view, these issues should be satisfyingly solved before propagating and establishing intensive husbandry systems for insects as a new type of mini-livestock factory farming.     

Cross-validation of the entomophagy attitude questionnaire (EAQ): A study in China on eaters and non-eaters

Research interest in direct entomophagy (the eating of insects) and indirect entomophagy (the eating of insects-fed animals) has grown exponentially in recent years. The use of insects as food and feed is a potential avenue to meet the growing global food demand, and for enhancing the sustainability of animal diets as well. The Entomophagy Attitude Questionnaire (EAQ) has been recently proposed as a specific measure of attitudes towards direct and indirect entomophagy. First aim of the current study was to cross-validate the EAQ in a non-Western country. An additional aim was to explore the differences between who have previously eaten insects and who have not. Hence, the EAQ was administered to a convenience Chinese sample (N = 395), together with measures of intention to eat insects either directly or indirectly. Confirmatory Factor Analysis showed a satisfactory fit of the EAQ three-factor model. The instrument showed adequate psychometric properties, and evidences of convergent and divergent validity of EAQ’s latent constructs were found. In addition, EAQ was found to predict effectively participants’ intention to engage both in direct and indirect entomophagy. Results thus support the validity of EAQ and the conceptualization underpinning it in a non-Western country. The main difference emerged between eaters and non-eaters regards the role of disgust, which is more predictive of intention for non-eaters, whereas positive attitude (interest) is more predictive for eaters. Hence, different communication strategies are suggested for individuals who already have vs. have not experienced the direct or indirect eating of insects.