Long-term sustained-released in situ gels of a water-insoluble drug amphotericin B for mycotic arthritis intra-articular administration: preparation,in vitro and in vivo evaluation

Amphotericin B (AMB) was often used in intra-articular injection administration for fungal arthritis, because it could often bring a satisfactory therapeutic efficacy and a minimum systemic toxic side effect. However, because of the multiple operations and the frequent injections, the compliance of the patients was bad. Therefore, to develop a long-term sustained-released preparation of AMB for mycotic arthritis intra-articular administration is of great significance. The purpose of present study was to develop a long-term sustained-released in situ gel of a water-insoluble drug AMB for mycotic arthritis intra-articular administration. Based on the evaluations of the in vitro properties of the formulations, the formulation containing 10% (w/w) ethanol, 15% (w/w) PG, 0.75% (w/w) HA, 5% (w/w) purified soybean oil, 0.03% (w/w) α-tocopherol, 15% (w/w) water and 55% (w/w) glyceryl monooleate was selected as a suitable intra-articular injectable in situ gel drug delivery system for water-insoluble drug AMB. Furthermore, the results of the in vivo study on rabbits showed that the selected formulation was a safe and effective long-term sustained-released intra-articular injectable AMB preparation. Therefore, the presented in situ AMB gel could reduce the frequency of the administration in the AMB treatment of fungal arthritis, and then would get a good patient compliance.     

苎麻织物的纤维素酶处理的研究

阐述了纤维素酶减量的作用原理，对苎麻织物的纤维素酶处理工艺和结果进行了讨论，确立了最佳工艺：酶用量2%owf，温度50～55℃，pH值5～5.5，苎麻织物用纤维素酶处理后结合用氨基硅微乳液处理，能改善外观、手感和悬垂性，基本解决刺痒感问题。     

Monoacylglycerols derived from butter oil by Penicillium roquefortii in suspension cultures

Structural isomers of monoacylglycerols (monoglycerides, MAGs) were identified and compared after degradation of butter oil by two strains of Penicillium roquefortii and a commercial lipase from P roquefortii (EC 3.1.1.3) at pH 7.0 and 10 °C. The conditions were selected as they were comparable with those used in the manufacture of blue mould‐ripened cheese. The commercial lipase was selected to compare with the fungal strains in terms of acyl migration. Results showed that the main isomers formed by lipolysis with the commercial lipase were sn‐2 MAGs (64 mol%), whilst spores and emerging mycelia of P roquefortii produced mainly sn‐1(3) MAGs (83–90 mol%). The work reported here may lead to further assessment of different MAG structural isomers as natural preservatives in foods and dairy products. © 2002 Society of Chemical Industry     

从烟草悬浮细胞中分离液泡前体的方法研究

通过跟踪含有液泡前体特异标记物的组分, 利用细胞壁酶水解胞壁制造原生质体, 并用真针头挤压的方法裂解细胞, 用蔗糖密度梯度离心的方法分离液泡前体. 通过相差显微镜观察, 发现所分离的液泡前体保持完整的结构. 利用不同细胞器的标记物抗体进行免疫标记检测, 证明所纯化的液泡前体不含其他细胞器, 具有较高的纯度.     

Investigation of the use of ram horn hydrolysate for fungal protein production

Production of the fungus Aspergillus niger NRRL 330 was studied in submerged fermentation with ram horn hydrolysate (RHH) as substrate. The characteristics of RHH have been reported previously. The RHH was enriched by addition of glucose and KH₂PO₄. The effects of kinetic parameters on the biomass yield of the fungus were investigated. The optimal conditions for growth of A niger on RHH were initial pH 6.5, temperature 30 °C, fermentation time 96 h and agitation speed 150 rpm. Under these optimal conditions the initial carbohydrate content of RHH was reduced from 1.52 to 0.2% and the biomass yield was 8.9 g l^?1. The biomass contained about 48.1% protein, 5.2% fat and 9.4% ash (on a dry weight basis). The amino acid content of the biomass was compared with Food and Agricultural Organisation (FAO) and animal feed standards. The protein produced contained all the essential amino acids for animal feed, but the amounts of these amino acids were somewhat lower than those of FAO and soybean reference protein. However, the amino acid composition of the biomass was better than that of animal feed. The results with RHH were also compared with previously reported data on fungal mycelium grown on waste liquor substrate. In conclusion, it was found that RHH could be used as a substrate in the production of fungal protein for use as animal feed. Copyright © 2003 Society of Chemical Industry     

Protein Extraction from Heat-stabilized Defatted Rice Bran: II. The Role of Amylase, Celluclast, and Viscozyme

ABSTRACT: The effectiveness of 3 carbohydrases for protein extraction from heat-stabilized defatted rice bran (HDRB) was evaluated. Amylase, viscozyme and celluclast extracted a maximum of 45.4, 12.1, and 28.5% protein, respectively. Further study showed that extracted protein ranged from 9.5 to 58.4% under conditions of water to bran ratio (5:1 to 20:1), α-amylase (0 to 110000 units/10 g rice bran), temperature (35 to 55 °C), and time (1 to 8 h). The maximum protein extracted was 58.4% with a water to bran ratio of 17:1, 87637 units amylase, and 50.9 °C. These results suggest that impure food-grade amylase containing protease is more effective than celluclast and viscozyme in protein extraction from HDRB.     

Metal sorption by biomass of melanin‐producing fungi grown in clay‐containing medium

The sorption of toxic metals by fungal mycelia grown in clay‐containing medium is reported in this work. Biomass of melanin‐producing microfungi of the genus Cladosporium (C cladosporioides, C resinae and C herbarum) and Aureobasidium pullulans, clay minerals and fungal mycelia grown in clay‐containing medium were compared for their equilibrium Cu and Cd uptake from pH‐buffered solutions using experimental sorption isotherms. Bentonite (Cherkassy) in the natural form was shown to be the best Cu sorbent compared with the other clays and clay minerals tested. Cu sorption isotherms fitted the Langmuir sorption model for all cases. Comparison of Cu and Cd uptake for biomass and bentonite showed a lower biomass uptake capacity but a considerably higher affinity of the biosorbent for the solute. The presence of kaolinite and palygorskite in the medium generally reduced both Cu and Cd sorption capacity and the metal‐binding ability of the fungal–clay mixtures. In contrast, addition of bentonite into the medium did not appreciably alter the Cd sorption ability but increased the sorption of Cu by A pullulans and C cladosporioides grown in this medium. A common feature for all fungi grown in the presence of bentonite was an increase in the Cu sorption capacity (Q_max) of the biomineral sorbents and a reduction in their affinity (Langmuir parameter b) compared with control biomass. A difference between predicted and experimental data obtained for biomass grown on bentonite medium was also observed. The connection between the sorption capacity of biomass grown in clay‐containing medium, mycelial morphology and the structure of fungal pellets is discussed and a mechanism for the changed sorption capacity of the combined biomineral sorbents is proposed which involves blocking or modification of binding sites on biotic and abiotic components of the ‘biomineral’ association. © 2002 Society of Chemical Industry     

纤维素酶分子的计算机辅助序列分析

利用生物大分子数据库（EMBL、SWALL和PDB）及软件技术（DNASIS和AntheProt）对嗜热子囊菌（Thermoascus aurantiacus)的产生的一种纤维素酶分子进行了序列分析及结构预测；通过多种来源的纤维素酶的比较研究，分析了其催化区、活性位点及局部保守序列。     

Improved cellulase production by stable Clostridium cellulolyticum mutants

Methods for the production of mutants of the cellulase producer Clostridium cellulolyticum ATCC 35319 were examined using an agar plate screening technique. Spontaneous and UV light-induced mutants were isolated, some of which exhibited a high level of both endoglucanase and exoglucanase activities as assayed using CMC (carboxymethyl cellulose) and PNPCb (paranitrophenyi-β-cellobioside) respectively. The volumetric enzyme activities were up to 2.5 times those of the wild strain.     

丝状真菌纤维素酶合成机制的研究

丝状真菌产生的纤维素酶被认为是最有应用前景的,但就目前酶生产效率来看,离实际应用还有很大的差距,需要对酶的合成调节机制有更为全面和深刻的了解。纤维素酶的生物合成受诱导和阻遏双重控制,酶的生产既有赖于低廉的保持一定浓度诱导物的存在,又必须清除分解代谢产物对酶合成的阻遏,其中的详细机制有待进一步的阐明。真菌纤维素酶的分泌也是相当复杂的,在酶的分泌过程中,酶会发生例如糖基化等一系列变化。提高纤维素酶的活力测定方法的准确性,使其进一步规范化,是研究纤维素酶工作的另外一个重要的领域。随着现代生物学朝着分子水平的不断发展,分子生物学的许多方法也越来越多地被应用于纤维素酶的研究中,并取得了重要进展。对真菌纤维素酶合成调节机制的深入研究,将为提高纤维素酶产量,推动应用工作的发展打下理论基础。