18个灵芝菌种的酯酶同工酶分析

对18个灵芝菌株进行了菌丝培养观察、拮抗试验和酯酶同工酶聚丙烯酰胺凝胶电泳实验，并对它们作了聚类统计分析。分析结果表明：在相似水平为50％时供试菌株聚合为6类：第1类包括以韩芝为代表的7个菌株；第2类包括以赤芝为代表的5个菌株；第3类包括紫芝和黑芝；第4类为血芝和京大灵芝；第5类为白芝；第6类为日本赤芝。聚类分析结果与形态特征及拮抗试验结果基本一致。实验还说明酯酶同工酶可作为灵芝良种选育的一种有效标记方法，在菌种鉴定、保藏和管理方面具有重要作用。     

圆唇散白蚁与五种蜚蠊酯酶同工酶的比较研究

用聚丙烯酰胺凝胶等电聚焦电泳技术对圆唇散白蚁的长翅成虫,工蚁、兵蚁与五种蜚蠊雌成虫的酯酶同工酶进行了比较研究.结果表明,圆唇散白蚁长翅成虫有11条酶带,工、兵蚁各有10条酶带.圆唇散白蚁与五种蜚蠊具4条共有酶带,其中,圆唇散白蚁与中华地鳖有8条共带,相似系数最高.酯酶同工酶可反映出等翅目与蜚蠊目昆虫的进化关系.     

大球盖菇提取物对CCl4所致急性肝损伤小鼠的抗氧化酶活性及同工酶的影响

目的：研究大球盖菇提取物(SRE)对小鼠肝和主要脏器的抗氧化酶活性及同工酶的影响。方法：小鼠连续30d 给予低(100mg/(kg bw·d))、中(200mg/(kg bw·d))、高(400mg/(kg bw·d))剂量SRE 后,灌胃给予CCl4(80mg/(kg bw·d))建立动物急性肝损伤模型,动物处死后取肝及主要脏器测定抗氧化酶活性,采用聚丙烯酰胺梯度凝胶电泳分析CAT 和SOD 同工酶。结果：与模型组比,高剂量SRE 能显著提高肝总SOD、Mn-SOD、CuZn-SOD 及CAT、GPx 活性。主要脏器抗氧化酶活性测定表明,给予SRE 后,心和肾SOD、CAT、GPx 活性比模型组显著提高;造模后肾Mn-SOD 表现出代偿性增高,给予SRE 后能使其恢复到对照组水平。各脏器CAT 同工酶都为1 条,在造模后酶带染色密度明显降低,在给予SRE 后能恢复到正常水平。肝有1 条Mn-SOD带和3 条CuZn-SOD 带,在给予SRE 后能使这些酶蛋白含量达到或高于正常水平;心、肾有1 条Mn-SOD 带和4 条CuZn-SOD 带,SRE 能增加心、肾CuZn-SOD 酶蛋白染色密度。结论：SRE 能通过提高抗氧化酶的活性和同工酶酶蛋白含量,有效预防急性肝损伤小鼠肝脏和主要脏器的氧化损伤作用。     

Oxa Analogues of Nexturastat A Demonstrate Improved HDAC6 Selectivity and Superior Antileukaemia Activity

The acetylome is important for maintaining the homeostasis of cells. Abnormal changes can result in the pathogenesis of immunological or neurological diseases, and degeneration can promote the manifestation of cancer. In particular, pharmacological intervention in the acetylome with pan-histone deacetylase (HDAC) inhibitors is clinically validated. However, these drugs exhibit an undesirable risk-benefit profile due to severe side effects. Selective HDAC inhibitors might promote patient compliance and represent a valuable opportunity in personalised medicine. Therefore, we envisioned the development of HDAC6-selective inhibitors. During our lead structure identification, we demonstrated that an alkoxyurea-based connecting unit proves to be beneficial for HDAC6 selectivity and established the synthesis of alkoxyurea-based hydroxamic acids. Herein, we report highly potent N-alkoxyurea-based hydroxamic acids with improved HDAC6 preference compared to nexturastat A. We further validated the biological activity of these oxa analogues of nexturastat A in a broad subset of leukaemia cell lines and demonstrated their superior anti-proliferative properties compared to nexturastat A.     

水稻生长锥离体发育过程的电镜扫描和同工酶初步分析

以水稻生长锥进行离体培养,用扫描电镜(SEM)观察了生长锥分化叶原基过程中生长锥的形态变化,并分析了生长锥中苹果酸脱氢酶同工酶的动态变化,对生长锥离体发育过程中同工酶变化与形态分化的关系进行了讨论。     

两种苦豆子生物碱对昆虫酯酶同功酶的影响

金省花碱和苦豆碱是从豆科植物苦豆子中分离出来的两种喹诺里西定类生物夏威夷 聚丙烯酰胺凝胶电泳分析，两种生物碱对酯酶同功酶有显著的抑制作用，可为分析其对昆虫的作用机制时参考。     

遗传多样性及其研究方法

在阐述遗传多样性的意义的基础上，对遗传多样性的研究方法从形态学、细胞、生化和DNA等水平分别进行了详细概述。     

Alcohol oxidase hybrid oligomers formed in vivo and in vitro

Cell‐free extract prepared from methanol‐grown cells of the methylotrophic yeast Pichia methanolica showed nine multiple alcohol oxidase (AOD) bands on active staining in native polyacrylamide gel electrophoresis. Their molecular basis was investigated and two AOD‐encoding genes, MOD1 and MOD2, were cloned from P. methanolica genome. When the two genes were expressed in a heterologous host, an alcohol oxidase‐depleted strain of Candida boidinii(aod1Δ strain), both MOD1 and MOD2 partially complemented growth defect of the host strain on methanol. While expression of either MOD1 or MOD2 in C. boidinii aod1Δ strain gave a single AOD band corresponding to the band with the largest and smallest mobility among the nine AOD bands, respectively, co‐expression of MOD1 and MOD2 resulted in multiple band formation. Mixed oligomerization of Mod1p and Mod2p in vitro also gave nine multiple bands. From these results, we concluded that the nine multiple forms of AOD observed on native–PAGE represent two homo‐octamers and seven hetero‐octamers of Mod1p and Mod2p. Using this zymogram analysis, we also found that Mod1p was preferably produced at low methanol concentrations in the media, while Mod2p was produced at higher methanol concentrations. This shows distinct regulatory features of the two AOD‐encoding genes in this methylotrophic yeast. Copyright © 1999 John Wiley & Sons, Ltd.     

冬瓜贮藏过程中PPO、POD和CAT活性及同功酶研究

研究了青皮冬瓜(Benincasa hispida)在贮藏过程中，其保护酶系统中POD和CAT以及PPO活性和同功酶电泳图谱变化。结果发现：腐烂前后冬瓜果肉中PPO活性升高，POD和CAT活性下降。电泳谱带分析发现，腐烂果肉中PPO的Rf0．19、POD的Rf0．17-Rf0．26Rf0．7以及CAT的Rf0．61条带出现了缺失。冬瓜果肉中未能检测到超氧化物歧化酶(SOD)活性。