超高效液相色谱串联质谱法测定烟草中的噁霉灵残留

为降低烟草中噁霉灵检测中的基质干扰，提高检测灵敏度和和稳定性，通过试验建立了以QuEChERS为样品前处理方法、液相色谱串联质谱仪为检测仪器的烟草中噁霉灵残留检测方法。样品加水浸润后，经丙酮提取、盐析试剂包除水、石墨化炭黑除杂后，采用Agilent ZORBAX 300SB-C8液相色谱柱（2.1 mm×100 mm，1.8 μm）分离，甲醇-水程序洗脱，ESI雾化，串联质谱MRM检测。结果发现，该方法在0.0025~0.0500 mg/L范围内线性良好，决定系数r²=0.9996，定量检测限1.06 μg/L，回收率在90.8%~92.0%之间，相对标准偏差为5.1%。运用该方法测定烟草中的噁霉灵，操作简单，检测精度高，满足CORESTA对烟草农残检测的要求。     

Cell Sheet Comprised of Mesenchymal Stromal Cells Overexpressing Stem Cell Factor Promotes Epicardium Activation and Heart Function Improvement in a Rat Model of Myocardium Infarction

Cell therapy of the post-infarcted myocardium is still far from clinical use. Poor survival of transplanted cells, insufficient regeneration, and replacement of the damaged tissue limit the potential of currently available cell-based techniques. In this study, we generated a multilayered construct from adipose-derived mesenchymal stromal cells (MSCs) modified to secrete stem cell factor, SCF. In a rat model of myocardium infarction, we show that transplantation of SCF producing cell sheet induced activation of the epicardium and promoted the accumulation of c-kit positive cells in ischemic muscle. Morphometry showed the reduction of infarct size (16%) and a left ventricle expansion index (0.12) in the treatment group compared to controls (24–28%; 0.17–0.32). The ratio of viable myocardium was more than 1.5-fold higher, reaching 49% compared to the control (28%) or unmodified cell sheet group (30%). Finally, by day 30 after myocardium infarction, SCF-producing cell sheet transplantation increased left ventricle ejection fraction from 37% in the control sham-operated group to 53%. Our results suggest that, combining the genetic modification of MSCs and their assembly into a multilayered construct, we can provide prolonged pleiotropic effects to the damaged heart, induce endogenous regenerative processes, and improve cardiac function.     

Bulging in incremental sheet forming of cold bonded multi-layered Cu clad sheet: Influence of forming conditions and bending

Bulge is a defect that causes geometrical inaccuracy and premature failure in the innovative incremental sheet forming (ISF) process. This study has two-fold objectives: (1) knowing the bulging behavior of a Cu clad tri-layered steel sheet as a function of forming conditions, and (2) analyzing the bending effect on bulging in an attempt to identify the associated mechanism. A series of ISF tests and bending analysis are performed to realize these objectives. From the cause-effect analysis, it is found that bulge formation in the layered sheet is sensitive to forming conditions in a way that bulging can be minimized utilizing annealed material and performing ISF with larger tool diameter and step size. The bending under tension analysis reveals that the formation of bulge is an outgrowth of bending moment that the forming tool applies on the sheet during ISF. Furthermore, the magnitude of bending moment depending upon the forming conditions varies from 0.046 to 10.24 N·m/m and causes a corresponding change in the mean bulge height from 0.07 to 0.91 mm. The bending moment governs bulging in layered sheet through a linear law. These findings lead to a conclusion that the bulge defect can be overcome by controlling the bending moment and the formula proposed can be helpful in this regards.     

轧制始极片

本文介绍了轧制法生产始极片的工艺，比较了轧制法和传统的钛种板生产始极片的优缺点，讨论了采用轧制始极片的经济效益和应用前景。     

抗乙肝病毒表面抗原PreS1(20-47)的单链抗体在转基因烟草根分泌物中的初步表达

为探索利用植物根分泌表达重组蛋白的可行性，构建了含有抗乙肝病毒表面抗原PreS1(20—47)单链抗体(ScFv)基因的表达载体。该ScFv基因转化烟草后在烟草根部细胞的细胞质和内质网中获得表达。实验结果表明，5’端融合ER导向信号肽的重组ScFv可通过根分泌表达。     

腐殖酸对烤烟根系生长和生理活性的影响

对腐殖酸和基肥混施进行了烤烟盆栽试验。结果表明 ,腐殖酸能有效刺激根系的生理活性 ,提高根系抗坏血酸氧化酶、多酚氧化酶等呼吸酶的活性 ,增强根系的活力 ,并能有效促进烤烟根系生长 ,根干重、体积和长度均有提高。     

硅钢片模具设计及其线切割加工

论述了电磁铁铁芯 (硅钢片 )模具的设计要点及其线切割加工的技巧 ,提出了提高模具加工精度和使用寿命的有效措施。     

Structural acrylic adhesives for the sheet steel fabrication industries

Recent developments in rubber-toughened structural acrylic adhesives now offer sheet steel fabricators the opportunity to replace conventional metal fastening techniques such as spot welding with lower cost, durable, more versatile structural adhesive bonding. These developments, in particular much improved ability to bond oily/waxy surfaces common to the motor car industry and improved retention of fracture toughness after exposure to paint stoving temperatures, are discussed in this paper.     

5754-H34铝合金板材生产工艺研究

研究了不同冷变形程度，退火温度对5754铝合金冷轧板材的组织和力学性能的影响，确定了5754合金H34状态板材的生产工艺参数，即冷变形程度25%-45￥，稳定化退火温度120-160℃，保温时间3-6h，在工业生产条件下，其产品的技术指标达到了用户要求。     

橡胶卷材挤出连续硫化生产线的结构特色与工作原理

介绍了橡胶防水防腐卷材挤出连续硫化生产线的结构、特点和工作原理;指出在目前国内同类设备中,该生产设备具有先进性,并可替代引进设备。