Tocol Composition and Supercritical Carbon Dioxide Extraction of Lipids from Barley Pearling Flour

Samples of whole grain and 35% pearling flour of 20 different barley varieties grown in Alberta were analyzed for their lipid contents. Total lipid contents of whole grains were within 1.9% to 3.0% (w/w), whereas those of the 35% pearling flour were 4.3% to 7.9%. Lipids of 35% pearling flour fraction of Tercel barley were extracted using supercritical carbon dioxide (SC‐CO₂) at different pressures (24, 45, and 58 MPa) and temperatures (40 and 60 °C) for 3 h. Lipid recoveries of 73% to 97% were achieved using SC‐CO₂ extraction under different operational conditions. Tocol contents and compositions of whole grain, 35% pearling flour, and SC‐CO₂ extracts were analyzed using HPLC. Tocol content of the whole grain was 53.8 to 124.9 μg/g and that of the pearling flour was 195 to 363 μg/g of flour. The hulless barley varieties were higher in tocols, with waxy, double waxy and Tercel varieties having the highest levels (P < 0.05). The ratios of total tocotrienols to total tocopherols varied within 1.6 to 3.9 range. Tocol concentrations of SC‐CO₂ extract fractions varied from 1171 to 4391 μg/g extract depending on the operational conditions. Barley oil is a good natural source of different tocol isomers rich in tocotrienols.     

乙二醇硬脂酸酯型珠光剂的合成及其应用研究

采用均匀实验设计,研究了硬脂酸和乙二醇酯化反应的合成工艺。以对甲苯磺酸和磷酸为复合酸催化剂,用量均为0.8%,硬脂酸∶乙二醇=1∶1.4(摩尔比),反应温度120℃条件下,硬脂酸的转化率为93.54%,合成的乙二醇硬脂酸酯珠光效应强,光泽较好。在表面活性剂体系粘度为200~240 mPa.s条件下,珠光剂的用量为1.0%~2.0%,搅拌速度控制在400~600 r/min,室温自然冷却得到的样品珠光光泽最优。     

Localization of phenolic acids and antioxidant activity in sorghum kernels

White and red of sorghum grains were sequentially pearled into 11 pearling fines and the corresponding 11 pearled kernels to study the localization of phenolic acids within sorghum grains. All fractions were analyzed for phenolic acids content by HPLC and antioxidant capacity by FRAP assays. Four phenolic acids identified in all fractions were caffeic, p-coumaric, ferulic, and sinapic acids. Data showed that the distribution of phenolic acids and antioxidant capacity in two sorghum genotypes was heterogeneous. Around 60% of the phenolic acids and the antioxidant capacity were recovered in the initial three pearling fine fractions that constituted about 20% surface removal. The concentrations of the phenolic acids decreased significantly with sequential pearling. Significant correlation between total phenolic acids and antioxidant capacity was observed with all fractions except for the first pearling fines from the red sorghum.     

Enrichment of Antioxidant Capacity and Vitamin E in Pita Made from Barley

This study aimed to enhance total antioxidant and vitamin E content of pita bread, by replacing 50% of the standard baker's flour with flours milled from covered (WI2585 and Harrington) or hulless (Finniss) barley genotypes, previously shown to have high antioxidant and vitamin E levels at harvest. Pita breads were made from either 100% baker's flour (control) or 50% malt flour, whole‐grain flour, or flour from barley grains pearled at 10%, 15%, and 20% grain weight. Antioxidant capacity and vitamin E content of flours and pitas were determined by their ability to scavenge 2,2‐diphenyl‐1‐picrylhydrazyl radicals and high performance liquid chromatography, respectively. The physical and sensory properties of the pitas were also assessed. All pitas made from either whole grain or pearled barley flour had a higher antioxidant capacity and most also had higher vitamin E content than standard pita. The antioxidant and vitamin E levels were reduced in pearled compared to whole grains, however the extent of that reduction varied among genotypes. The greatest antioxidant and vitamin E levels were found in pita made from malt flour or Finniss whole grain flour. Furthermore, sensory analysis suggested these pitas were acceptable to consumers and retained similar physical and sensory properties to those in the control pita.     

Concentrations of functional lipids in abraded fractions of hulless barley and effect of storage

Hulless barley kernels were sequentially abraded to achieve 4%, 8%, 16%, 24%, 32%, and 40% removal. Abraded fines, kernels, and ground kernels were stored at 35 degrees C and 75% relative humidity for 3 wk. Stored samples were extracted and the levels of oil, free phytosterols, tocopherols (Ts), and tocotrienols (T3s) were analyzed and compared with freshly abraded fractions. The results revealed that oil, sterols, and Ts were concentrated in the outer layers, particularly in the germ layer. In whole kernels, homologues of both Ts and T3s showed the same ranking order in concentrations as alpha > gamma > beta > delta. The homologue composition of Ts remained the same but that of T3s changed across the kernel. The %T3 in total tocols increased in fractions with increasing endosperm tissue. Storage caused no change in oil and Ts but significant changes in sterols and T3s. The changes were differential among T3 isomers, with alpha-T3 decreasing and delta-T3 increasing. The degradation of alpha-T3 was accelerated in fractions with more endosperm tissue. Grinding kernel samples before storage accelerated sterol degradation but had a limited effect on changes of T3s. A 2nd experiment using a different hulless barley line and ambient storage for 6 mo confirmed all the findings except that the changing trend for sterols was inconsistent. These results provide practical information to those who wish to produce a barley fraction enriched with a particular functional lipid and maintain stability of their products.     

The Measurement of Structural Characteristics of Barley for Shochu Using Single‐Kernel Characterization System 4100 Crush‐Response Profiles

The selection of grain that is optimum for barley Shochu is not straightforward. Usually malting barley is used, but not all malting barley samples perform well in Shochu production. In addition there is a genotype × environment effect, so that a given variety may perform satisfactorily in one environment but not another. Therefore a method for testing samples for Shochu is required. This group has previously reported on the evaluation of barley for Shochu production using the Single Kernel Characterization System (SKCS) and demonstrated a significant correlation between the SKCS Hardness Index and pearling performance. The Hardness Index values are multivariate statistical predictions derived from an incremental change analysis of the crush‐response profiles. In the present study, a detailed analysis was performed of the averaged SKCS Crush‐Response Profiles of barley, obtained when measuring the SKCS Hardness Index. It was concluded that the morphological differences between averaged barley SKCS Crush‐Response Profiles appear to allow barley varieties to be classified into four distinct classes. In addition, the elastic response to the crushing on the SKCS device of the aleurone layers of barley kernels yields a useful predictor of pearling quality/performance.     

Evaluating quality of yellow alkaline noodles made from mechanically abraded sprouted wheat

Five hard red spring wheat genotypes, with initial falling numbers (FNs) ranging from 62 to 427 s, were mechanically abraded for 30, 60, and 120 s to reduce sequentially α‐amylase activity in the grain. Mechanical abrasion prior to milling significantly increased FN and reduced amylase activity in pre‐harvest sprouted wheat. The abraded grains were milled and the resultant flour samples were evaluated for starch gelatinization properties and yellow alkaline noodle (YAN) quality. Mechanical abrasion on sprouted kernels resulted in significant improvements in starch pasting properties accompanied by marginal decreases in flour ash and protein contents. Initial noodle brightness L* improved in most samples with mechanical abrasion. However, L* values dropped significantly during storage, with the greatest decline seen in the highly sprouted sample. Noodle cooking and textural qualities were relatively unaffected by sprout damage or mechanical abrasion, possibly due to amylase inhibition by the carbonate salts present in the formula. Mechanical abrasion prior to milling was found to reduce amylase activity significantly in moderately sprouted wheat. YAN, however, showed high tolerance to sprout damage, and produced noodles of acceptable texture without mechanical abrasion. Copyright © 2003 Society of Chemical Industry     

Comparison of lipid content and fatty acid composition and their distribution within seeds of 5 small grain species

Barley, oats, rice, sorghum, and wheat, each with two genotypes, were sequentially abraded by an electric seed scarifier. The pearling fines (PF) and pearled kernels (PK) at each cycle were analyzed for lipid (mostly nonpolar) content and fatty acid (FA) composition. The oil content in whole or dehulled grains ranged from 2.18% of a wheat variety to 6.38% of an oat line. Compared with barley and wheat, rice, oat, and sorghum had higher relative % of C18:1 (31.60 to 36.64 compared with 12.15 to 15.61) and lower % of C18:2 (35.69 to 45.44 compared with 50.79 to 61.50). The relationship between oil content in PF and the cumulative level of surface removal essentially describes the distribution pattern of oil content within a seed. Barley, rice, and sorghum had a similar distribution pattern, characterized by a rapid rate of decreasing for the first few outer layers and then by gradual decrease to a flat value toward the inner core. In contrast, distribution within oats was characterized by a gradual reduction in oil content across the seed. The distribution of oil within wheat fell between the former 2 types. For all 10 grains, from seed surface to inner core, C16:0 and C18:0 increased, C18:1 and C18:3 decreased, and C18:2 changed slightly, providing a new reason for improved oxidative stability for pearled kernels. The differences in the changing intensity of FA composition among grain species correspond to those in oil distribution within a seed, while varietal difference in distribution patterns of content and FA composition of lipids within a species was insignificant. PRACTICAL APPLICATION: This study was the first to document fatty acid distribution across a grain seed. Results provide 2 major reasons for improved oxidative stability of pearled grains: reduced oil content and shift of fatty acids toward more saturated and less unsaturated composition.     

Effect of the Variety of Barley and Pearling Rate on the Quality of Shochu Koji

In liquor production, barley koji is used a source of saccharification enzymes, nutrition for yeast multiplication, and to impart flavor to the shochu product. Its role is analogous to that of malt in beer and whisky production. In this report, the influence of barley variety and pearling rate on barley koji characteristics is shown. Four Australian varieties (Stirling, Schooner, Arapiles, Grimmett) and one Japanese variety (Nishinohoshi) were evaluated. Significant differences were observed among barley varieties and pearling rate with respect to the amount of koji mycelia produced, enzyme activity and the amino acid composition of the barley koji.