新生牛肝活性肽的制备及其毒性检测

目的制备新生牛肝活性肽并评价其安全性。方法采用膜法分离制备新生牛肝活性肽。将3批制品于37～40℃,75%相对湿度条件下存放3个月,以多肽含量为指标观察其稳定性,并进行急性毒性试验、小鼠骨髓细胞微核试验、Ames试验、小鼠精子畸形试验和大鼠喂养试验。结果制备的3批新生牛肝活性肽存放3个月后,多肽含量无明显下降,质量稳定。小鼠和大鼠经口灌人大于20.0g/kg体重的新生牛肝活性肽,均无急性毒性。3种致突变试验均未显示出致突变性,大鼠喂养试验各项指标均未见明显毒性。结论新生牛肝活性肽未表现出明显毒性。     

Purification and Characterization of an Endoprotease from Melon Fruit

An endoprotease was purified from melon fruit (Cucumis melo L.) by ammonium sulfate precipitation, gel filtration and ion-exchange chromatography using t-butyloxycarbonyl-Ala-Ala-Pro-Leu p-nitroanilide as a substrate. The molecular weight was estimated as 26,000 and isoelectric point pH 9.5. It preferentially hydrolyzed peptide bonds of the carboxyl terminal sides of Leu, Ala, His, Gin, and Am. Activity was strongly inhibited by diisopropyl phosphofluoridate, indicating the serine protease nature of the enzyme. The migration distance on electrophoresis, molecular weight and substrate specificity differed from cucumisin, a known protease from melon. This unusual protease may have potential for special food treatment applications.     

谷氨酰胺(Gln)活性肽营养液的研究进展

对G1n小肽在氨基酸营养液中的使用以及G1n小肽的稳定性和代谢机制进行了论述。G1n是一种“条件必需氨基酸”，但Cln的水溶液不稳定，因而在氨基酸输液或氨基酸营养被中，用G1n小肽代替G1n单体使用。G1n小肽含有取代的α—NH2，使Cln小肽的稳定性大大增加。     

The Neurovascular Unit Dysfunction in Alzheimer’s Disease

Alzheimer’s disease (AD) is the most common neurodegenerative disease worldwide. Histopathologically, AD presents with two hallmarks: neurofibrillary tangles (NFTs), and aggregates of amyloid β peptide (Aβ) both in the brain parenchyma as neuritic plaques, and around blood vessels as cerebral amyloid angiopathy (CAA). According to the vascular hypothesis of AD, vascular risk factors can result in dysregulation of the neurovascular unit (NVU) and hypoxia. Hypoxia may reduce Aβ clearance from the brain and increase its production, leading to both parenchymal and vascular accumulation of Aβ. An increase in Aβ amplifies neuronal dysfunction, NFT formation, and accelerates neurodegeneration, resulting in dementia. In recent decades, therapeutic approaches have attempted to decrease the levels of abnormal Aβ or tau levels in the AD brain. However, several of these approaches have either been associated with an inappropriate immune response triggering inflammation, or have failed to improve cognition. Here, we review the pathogenesis and potential therapeutic targets associated with dysfunction of the NVU in AD.     

The effect of reaction conditions on montmorillonite-catalysed peptide formation

Oligomerization of glycine (gly) and diglycine (gly₂) on montmorillonite was performed as cyclic, drying-wetting process at temperatures below 100°C, under varying reaction conditions. The influence of substrate/clay ratio, temperature and pH was found to be different for amino acid (AA) dimerization, cyclic anhydride (CA) formation and peptide chain elongation. High temperatures and neutral pH favour CA formation over diglycine production. An AA/catalyst ratio of 0.2 mmol/g leads to optimal yields. This supports the assumption that amino acid dimerization and CA formation take place at the edges of clay particles. Peptide chain elongation, starting from gly₂, produces higher yields at higher temperatures and neutral pH.     

肽类消化吸收方式与机制研究进展

最新研究表明,寡肽可以被小肠直接吸收,但其吸收方式是与氨基酸不同的、相互独立的转运机制。寡肽的特殊载体,通过氢离子和钙离子浓度电导的消耗能量的主动转运系统、具pH依赖性的非耗能性钠离子和氢离子交换系统和谷胱甘肽（GSH）等三种系统进行转运。食物蛋白质的品质、肽链长度与肽的氨基酸组成、生理状况等因素都会影响寡肽的消化吸收。     

丝肽预处理对棉织物天然染料染色性能的影响

以柞蚕落绵为原料制备丝肽,并对其进行红外及紫外光谱分析.采用该丝肽整理棉织物,然后用艾蒿、苏木、红茶3种天然染料对整理前后的棉织物进行染色,探讨丝肽整理对棉织物天然染料染色性能的影响.结果表明:经丝肽整理后,棉织物采用3种天然染料染色的K/S值均有不同程度的提高,摩擦牢度和皂洗牢度略有下降,其中红茶Cu2+媒染样品的K/S值提高最显著,为整理前的2.24倍,其皂洗和摩擦牢度均达到3级以上.棉织物经丝肽整理后,断裂强力稍有下降,白度基本保持不变,柔软度有所改善.     

Chemical synthesis of {gamma}-echistatin analogues: elucidation of status of C-terminal (45-49)

Three analogues of -echistatin, des(45–49)--echistatin,des(46–49)-y-echistatin and des(47–49)--echistatin,were synthesized by solid-phase methodology and their biologicalactivities were measured and compared. The results reveal thatwithout the C-terminal (45–49) of -echistatin, the foldingof the protein to the final active structure is not interferedwith and Lys-45 influences the inhibition of platelet aggregation.     

一种靶向蛋白质超声微泡的制备及表征

以氟碳气体为超声造影剂,通过接枝纳米磁性颗粒-RGD多肽,构建靶向蛋白质超声微泡,制备一种可同时用于超声造影和磁共振成像(MRI)的双模态造影增强剂。通过显微镜对磁性蛋白超声微泡形貌进行观察,利用红外光谱、分光光度法分析纳米磁性颗粒接枝牛血清蛋白的接枝率,并通过细胞实验评价磁性蛋白微泡的生物相容性。